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Protein

Proteasome subunit alpha type-2-B

Gene

PAB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-4641258. Degradation of DVL.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5668541. TNFR2 non-canonical NF-kB pathway.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-2-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit B-2
Gene namesi
Name:PAB2
Ordered Locus Names:At1g79210
ORF Names:YUP8H12R.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G79210.
TAIRilocus:2207340. AT1G79210.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001240861 – 235Proteasome subunit alpha type-2-BAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ8L4A7.
PRIDEiQ8L4A7.

PTM databases

iPTMnetiQ8L4A7.

Expressioni

Gene expression databases

ExpressionAtlasiQ8L4A7. baseline and differential.
GenevisibleiQ8L4A7. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi29481. 2 interactors.
STRINGi3702.AT1G79210.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L4A7.
SMRiQ8L4A7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0181. Eukaryota.
ENOG410XPQ8. LUCA.
HOGENOMiHOG000091085.
KOiK02726.
OMAiMNELAYS.
OrthoDBiEOG09360KM5.
PhylomeDBiQ8L4A7.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR023332. Proteasome_alpha-type.
IPR000426. Proteasome_asu_N.
IPR001353. Proteasome_sua/b.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
SMARTiView protein in SMART
SM00948. Proteasome_A_N. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8L4A7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSQYSFSL TTFSPSGKLV QIEHALTAVG SGQTSLGIKA SNGVVIATEK
60 70 80 90 100
KLPSILVDEA SVQKIQHLTP NIGTVYSGMG PDFRVLVRKS RKQAEQYLRL
110 120 130 140 150
YKEPIPVTQL VRETATVMQE FTQSGGVRPF GVSLLVAGYD DKGPQLYQVD
160 170 180 190 200
PSGSYFSWKA SAMGKNVSNA KTFLEKRYTE DMELDDAIHT AILTLKEGFE
210 220 230
GEISSKNIEI GKIGTDKVFR VLTPAEIDDY LAEVE
Length:235
Mass (Da):25,733
Last modified:October 1, 2002 - v1
Checksum:i9B2DA84B57FC8B95
GO

Sequence cautioni

The sequence AAC17043 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002986 Genomic DNA. Translation: AAC17043.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36215.1.
CP002684 Genomic DNA. Translation: AEE36216.1.
CP002684 Genomic DNA. Translation: AEE36217.1.
AY094433 mRNA. Translation: AAM19806.1.
AY122893 mRNA. Translation: AAM67426.1.
PIRiT01036.
RefSeqiNP_001077845.1. NM_001084376.1.
NP_001077846.1. NM_001084377.1.
NP_178042.1. NM_106572.6.
UniGeneiAt.10591.

Genome annotation databases

EnsemblPlantsiAT1G79210.1; AT1G79210.1; AT1G79210.
AT1G79210.2; AT1G79210.2; AT1G79210.
AT1G79210.3; AT1G79210.3; AT1G79210.
GeneIDi844262.
GrameneiAT1G79210.1; AT1G79210.1; AT1G79210.
AT1G79210.2; AT1G79210.2; AT1G79210.
AT1G79210.3; AT1G79210.3; AT1G79210.
KEGGiath:AT1G79210.

Similar proteinsi

Entry informationi

Entry nameiPSA2B_ARATH
AccessioniPrimary (citable) accession number: Q8L4A7
Secondary accession number(s): O64532
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2002
Last modified: September 27, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families