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Protein

3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial

Gene

KAS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Enzyme regulationi

Inhibited by cerulenin.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei209PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT2G04540-MONOMER.
MetaCyc:AT2G04540-MONOMER.
BRENDAi2.3.1.41. 399.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl-ACP synthase
mtKAS
Gene namesi
Name:KAS
Ordered Locus Names:At2g04540
ORF Names:T1O3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G04540.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Slow growth and bleached leaf phenotype when grown under ambient air, but normal growth under CO(2)-enriched air. Highly prevents lipoylation of the H-protein subunit of the glycine decarboxylase (GDC) in leaves, but has only a limited effect on the lipoylation of the E2 subunits of pyruvate dehydrogenase (PDH) and alpha-ketoacid dehydrogenase (KGDH) complexes in leaves and even no effect in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionSequence analysisAdd BLAST28
ChainiPRO_000000058829 – 4613-oxoacyl-[acyl-carrier-protein] synthase, mitochondrialAdd BLAST433

Proteomic databases

PaxDbiQ8L3X9.
PRIDEiQ8L3X9.

PTM databases

iPTMnetiQ8L3X9.

Expressioni

Tissue specificityi

Expressed at the same level in leaves, roots and flowers.1 Publication

Gene expression databases

GenevisibleiQ8L3X9. AT.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G04540.1.

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 42Combined sources10
Beta strandi45 – 47Combined sources3
Helixi48 – 56Combined sources9
Beta strandi62 – 64Combined sources3
Helixi67 – 70Combined sources4
Helixi77 – 85Combined sources9
Beta strandi91 – 93Combined sources3
Beta strandi97 – 100Combined sources4
Helixi106 – 109Combined sources4
Helixi117 – 132Combined sources16
Helixi140 – 144Combined sources5
Beta strandi146 – 154Combined sources9
Helixi157 – 168Combined sources12
Helixi172 – 174Combined sources3
Helixi179 – 183Combined sources5
Helixi187 – 196Combined sources10
Helixi208 – 210Combined sources3
Helixi211 – 225Combined sources15
Beta strandi229 – 237Combined sources9
Helixi242 – 250Combined sources9
Turni257 – 260Combined sources4
Helixi262 – 264Combined sources3
Beta strandi281 – 289Combined sources9
Helixi290 – 295Combined sources6
Beta strandi302 – 311Combined sources10
Helixi324 – 337Combined sources14
Helixi341 – 343Combined sources3
Beta strandi346 – 348Combined sources3
Helixi355 – 369Combined sources15
Helixi371 – 374Combined sources4
Beta strandi378 – 381Combined sources4
Helixi384 – 387Combined sources4
Helixi391 – 393Combined sources3
Helixi394 – 408Combined sources15
Beta strandi418 – 420Combined sources3
Beta strandi426 – 428Combined sources3
Beta strandi440 – 448Combined sources9
Turni449 – 451Combined sources3
Beta strandi452 – 459Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W0IX-ray2.10A/B31-461[»]
2IX4X-ray1.95A/B31-461[»]
ProteinModelPortaliQ8L3X9.
SMRiQ8L3X9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8L3X9.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi139 – 144Poly-Glu6

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1394. Eukaryota.
COG0304. LUCA.
HOGENOMiHOG000060166.
InParanoidiQ8L3X9.
KOiK09458.
OMAiQKASRPY.
OrthoDBiEOG093609NZ.
PhylomeDBiQ8L3X9.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SMARTiSM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L3X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSNLRRHL SASRLRLNRF ISTSSSYHSH RRVVVTGLGM VTPLGRGVET
60 70 80 90 100
TWRRLIDGEC GIRGLTLDDL KMKSFDEETK LYTFDQLSSK VAAFVPYGSN
110 120 130 140 150
PGEFDEALWL NSKAVANFIG YAVCAADEAL RDAEWLPTEE EEKERTGVSI
160 170 180 190 200
GGGIGSICDI VEAAQLICEK RLRRLSPFFI PKILVNMASG HVSMKYGFQG
210 220 230 240 250
PNHAAVTACA TGAHSIGDAT RMIQFGDADV MVAGGTESSI DALSVAGFSR
260 270 280 290 300
SRALSTKFNS SPQEASRPFD CDRDGFVIGE GSGVIVLEEY EHAKRRGAKI
310 320 330 340 350
YAELCGYGMS GDAHHITQPP EDGKGAVLAM TRALRQSGLC PNQIDYVNAH
360 370 380 390 400
ATSTPIGDAV EARAIKTVFS EHATSGTLAF SSTKGATGHL LGAAGAVEAI
410 420 430 440 450
FSILAIHHGV APMTLNVKNP DPIFDKRFMP LTTSKKMLVR TAMSNSFGFG
460
GTNASLLFAS I
Length:461
Mass (Da):49,379
Last modified:October 1, 2002 - v1
Checksum:iD9B02B19F5A5FB45
GO

Sequence cautioni

The sequence AAD25826 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073746 mRNA. Translation: BAB91181.1.
AC006951 Genomic DNA. Translation: AAD25826.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05844.1.
AY099587 mRNA. Translation: AAM20439.1.
AY128832 mRNA. Translation: AAM91232.1.
PIRiF84458.
RefSeqiNP_178533.2. NM_126485.4.
UniGeneiAt.41299.

Genome annotation databases

EnsemblPlantsiAT2G04540.1; AT2G04540.1; AT2G04540.
GeneIDi814996.
GrameneiAT2G04540.1; AT2G04540.1; AT2G04540.
KEGGiath:AT2G04540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073746 mRNA. Translation: BAB91181.1.
AC006951 Genomic DNA. Translation: AAD25826.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05844.1.
AY099587 mRNA. Translation: AAM20439.1.
AY128832 mRNA. Translation: AAM91232.1.
PIRiF84458.
RefSeqiNP_178533.2. NM_126485.4.
UniGeneiAt.41299.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1W0IX-ray2.10A/B31-461[»]
2IX4X-ray1.95A/B31-461[»]
ProteinModelPortaliQ8L3X9.
SMRiQ8L3X9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G04540.1.

PTM databases

iPTMnetiQ8L3X9.

Proteomic databases

PaxDbiQ8L3X9.
PRIDEiQ8L3X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G04540.1; AT2G04540.1; AT2G04540.
GeneIDi814996.
GrameneiAT2G04540.1; AT2G04540.1; AT2G04540.
KEGGiath:AT2G04540.

Organism-specific databases

TAIRiAT2G04540.

Phylogenomic databases

eggNOGiKOG1394. Eukaryota.
COG0304. LUCA.
HOGENOMiHOG000060166.
InParanoidiQ8L3X9.
KOiK09458.
OMAiQKASRPY.
OrthoDBiEOG093609NZ.
PhylomeDBiQ8L3X9.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT2G04540-MONOMER.
MetaCyc:AT2G04540-MONOMER.
BRENDAi2.3.1.41. 399.

Miscellaneous databases

EvolutionaryTraceiQ8L3X9.
PROiQ8L3X9.

Gene expression databases

GenevisibleiQ8L3X9. AT.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SMARTiSM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKASM_ARATH
AccessioniPrimary (citable) accession number: Q8L3X9
Secondary accession number(s): Q9SJB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mitochondrial protein lipoylation in leaves does not exclusively depend on the lipoate biosynthesis by KAS and may occur independently of this pathway in roots.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.