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Q8L3X9

- KASM_ARATH

UniProt

Q8L3X9 - KASM_ARATH

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Protein
3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial
Gene
KAS, At2g04540, T1O3.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Enzyme regulationi

Inhibited by cerulenin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei209 – 2091 By similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT2G04540-MONOMER.
MetaCyc:AT2G04540-MONOMER.
BRENDAi2.3.1.41. 399.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl-ACP synthase
mtKAS
Gene namesi
Name:KAS
Ordered Locus Names:At2g04540
ORF Names:T1O3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G04540.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Slow growth and bleached leaf phenotype when grown under ambient air, but normal growth under CO(2)-enriched air. Highly prevents lipoylation of the H-protein subunit of the glycine decarboxylase (GDC) in leaves, but has only a limited effect on the lipoylation of the E2 subunits of pyruvate dehydrogenase (PDH) and alpha-ketoacid dehydrogenase (KGDH) complexes in leaves and even no effect in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828Mitochondrion Reviewed prediction
Add
BLAST
Chaini29 – 4614333-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial
PRO_0000000588Add
BLAST

Proteomic databases

PaxDbiQ8L3X9.
PRIDEiQ8L3X9.

Expressioni

Tissue specificityi

Expressed at the same level in leaves, roots and flowers.1 Publication

Gene expression databases

GenevestigatoriQ8L3X9.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G04540.1-P.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 4210
Beta strandi45 – 473
Helixi48 – 569
Beta strandi62 – 643
Helixi67 – 704
Helixi77 – 859
Beta strandi91 – 933
Beta strandi97 – 1004
Helixi106 – 1094
Helixi117 – 13216
Helixi140 – 1445
Beta strandi146 – 1549
Helixi157 – 16812
Helixi172 – 1743
Helixi179 – 1835
Helixi187 – 19610
Helixi208 – 2103
Helixi211 – 22515
Beta strandi229 – 2379
Helixi242 – 2509
Turni257 – 2604
Helixi262 – 2643
Beta strandi281 – 2899
Helixi290 – 2956
Beta strandi302 – 31110
Helixi324 – 33714
Helixi341 – 3433
Beta strandi346 – 3483
Helixi355 – 36915
Helixi371 – 3744
Beta strandi378 – 3814
Helixi384 – 3874
Helixi391 – 3933
Helixi394 – 40815
Beta strandi418 – 4203
Beta strandi426 – 4283
Beta strandi440 – 4489
Turni449 – 4513
Beta strandi452 – 4598

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W0IX-ray2.10A/B31-461[»]
2IX4X-ray1.95A/B31-461[»]
ProteinModelPortaliQ8L3X9.
SMRiQ8L3X9. Positions 31-461.

Miscellaneous databases

EvolutionaryTraceiQ8L3X9.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi139 – 1446Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0304.
HOGENOMiHOG000060166.
InParanoidiQ8L3X9.
KOiK09458.
OMAiHAQKRNA.
PhylomeDBiQ8L3X9.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8L3X9-1 [UniParc]FASTAAdd to Basket

« Hide

MATSNLRRHL SASRLRLNRF ISTSSSYHSH RRVVVTGLGM VTPLGRGVET    50
TWRRLIDGEC GIRGLTLDDL KMKSFDEETK LYTFDQLSSK VAAFVPYGSN 100
PGEFDEALWL NSKAVANFIG YAVCAADEAL RDAEWLPTEE EEKERTGVSI 150
GGGIGSICDI VEAAQLICEK RLRRLSPFFI PKILVNMASG HVSMKYGFQG 200
PNHAAVTACA TGAHSIGDAT RMIQFGDADV MVAGGTESSI DALSVAGFSR 250
SRALSTKFNS SPQEASRPFD CDRDGFVIGE GSGVIVLEEY EHAKRRGAKI 300
YAELCGYGMS GDAHHITQPP EDGKGAVLAM TRALRQSGLC PNQIDYVNAH 350
ATSTPIGDAV EARAIKTVFS EHATSGTLAF SSTKGATGHL LGAAGAVEAI 400
FSILAIHHGV APMTLNVKNP DPIFDKRFMP LTTSKKMLVR TAMSNSFGFG 450
GTNASLLFAS I 461
Length:461
Mass (Da):49,379
Last modified:October 1, 2002 - v1
Checksum:iD9B02B19F5A5FB45
GO

Sequence cautioni

The sequence AAD25826.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB073746 mRNA. Translation: BAB91181.1.
AC006951 Genomic DNA. Translation: AAD25826.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05844.1.
AY099587 mRNA. Translation: AAM20439.1.
AY128832 mRNA. Translation: AAM91232.1.
PIRiF84458.
RefSeqiNP_178533.2. NM_126485.3.
UniGeneiAt.41299.

Genome annotation databases

EnsemblPlantsiAT2G04540.1; AT2G04540.1; AT2G04540.
GeneIDi814996.
KEGGiath:AT2G04540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB073746 mRNA. Translation: BAB91181.1 .
AC006951 Genomic DNA. Translation: AAD25826.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC05844.1 .
AY099587 mRNA. Translation: AAM20439.1 .
AY128832 mRNA. Translation: AAM91232.1 .
PIRi F84458.
RefSeqi NP_178533.2. NM_126485.3.
UniGenei At.41299.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W0I X-ray 2.10 A/B 31-461 [» ]
2IX4 X-ray 1.95 A/B 31-461 [» ]
ProteinModelPortali Q8L3X9.
SMRi Q8L3X9. Positions 31-461.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G04540.1-P.

Proteomic databases

PaxDbi Q8L3X9.
PRIDEi Q8L3X9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G04540.1 ; AT2G04540.1 ; AT2G04540 .
GeneIDi 814996.
KEGGi ath:AT2G04540.

Organism-specific databases

TAIRi AT2G04540.

Phylogenomic databases

eggNOGi COG0304.
HOGENOMi HOG000060166.
InParanoidi Q8L3X9.
KOi K09458.
OMAi HAQKRNA.
PhylomeDBi Q8L3X9.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci ARA:AT2G04540-MONOMER.
MetaCyc:AT2G04540-MONOMER.
BRENDAi 2.3.1.41. 399.

Miscellaneous databases

EvolutionaryTracei Q8L3X9.
PROi Q8L3X9.

Gene expression databases

Genevestigatori Q8L3X9.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000447. KAS_II. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
TIGRFAMsi TIGR03150. fabF. 1 hit.
PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular characterization of the beta-ketoacyl-[acyl carrier-protein] synthase component of the Arabidopsis mitochondrial fatty acid synthase."
    Yasuno R., von Wettstein-Knowles P., Wada H.
    J. Biol. Chem. 279:8242-8251(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Mitochondrial protein lipoylation does not exclusively depend on the mtKAS pathway of de novo fatty acid synthesis in Arabidopsis."
    Ewald R., Kolukisaoglu U., Bauwe U., Mikkat S., Bauwe H.
    Plant Physiol. 145:41-48(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Structure of the mitochondrial beta-ketoacyl-[acyl carrier-protein] synthase from Arabidopsis and its role in fatty acid synthesis."
    Olsen J.G., Rasmussen A.V., von Wettstein-Knowles P., Henriksen A.
    FEBS Lett. 577:170-174(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 31-461, SUBUNIT.

Entry informationi

Entry nameiKASM_ARATH
AccessioniPrimary (citable) accession number: Q8L3X9
Secondary accession number(s): Q9SJB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mitochondrial protein lipoylation in leaves does not exclusively depend on the lipoate biosynthesis by KAS and may occur independently of this pathway in roots (1 Publication).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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