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Q8L3X9

- KASM_ARATH

UniProt

Q8L3X9 - KASM_ARATH

Protein

3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial

Gene

KAS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins.1 Publication

    Catalytic activityi

    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

    Enzyme regulationi

    Inhibited by cerulenin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei209 – 2091PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT2G04540-MONOMER.
    MetaCyc:AT2G04540-MONOMER.
    BRENDAi2.3.1.41. 399.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial (EC:2.3.1.41)
    Alternative name(s):
    Beta-ketoacyl-ACP synthase
    mtKAS
    Gene namesi
    Name:KAS
    Ordered Locus Names:At2g04540
    ORF Names:T1O3.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G04540.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Slow growth and bleached leaf phenotype when grown under ambient air, but normal growth under CO(2)-enriched air. Highly prevents lipoylation of the H-protein subunit of the glycine decarboxylase (GDC) in leaves, but has only a limited effect on the lipoylation of the E2 subunits of pyruvate dehydrogenase (PDH) and alpha-ketoacid dehydrogenase (KGDH) complexes in leaves and even no effect in roots.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828MitochondrionSequence AnalysisAdd
    BLAST
    Chaini29 – 4614333-oxoacyl-[acyl-carrier-protein] synthase, mitochondrialPRO_0000000588Add
    BLAST

    Proteomic databases

    PaxDbiQ8L3X9.
    PRIDEiQ8L3X9.

    Expressioni

    Tissue specificityi

    Expressed at the same level in leaves, roots and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ8L3X9.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT2G04540.1-P.

    Structurei

    Secondary structure

    1
    461
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 4210
    Beta strandi45 – 473
    Helixi48 – 569
    Beta strandi62 – 643
    Helixi67 – 704
    Helixi77 – 859
    Beta strandi91 – 933
    Beta strandi97 – 1004
    Helixi106 – 1094
    Helixi117 – 13216
    Helixi140 – 1445
    Beta strandi146 – 1549
    Helixi157 – 16812
    Helixi172 – 1743
    Helixi179 – 1835
    Helixi187 – 19610
    Helixi208 – 2103
    Helixi211 – 22515
    Beta strandi229 – 2379
    Helixi242 – 2509
    Turni257 – 2604
    Helixi262 – 2643
    Beta strandi281 – 2899
    Helixi290 – 2956
    Beta strandi302 – 31110
    Helixi324 – 33714
    Helixi341 – 3433
    Beta strandi346 – 3483
    Helixi355 – 36915
    Helixi371 – 3744
    Beta strandi378 – 3814
    Helixi384 – 3874
    Helixi391 – 3933
    Helixi394 – 40815
    Beta strandi418 – 4203
    Beta strandi426 – 4283
    Beta strandi440 – 4489
    Turni449 – 4513
    Beta strandi452 – 4598

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W0IX-ray2.10A/B31-461[»]
    2IX4X-ray1.95A/B31-461[»]
    ProteinModelPortaliQ8L3X9.
    SMRiQ8L3X9. Positions 31-461.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8L3X9.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi139 – 1446Poly-Glu

    Sequence similaritiesi

    Belongs to the beta-ketoacyl-ACP synthases family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0304.
    HOGENOMiHOG000060166.
    InParanoidiQ8L3X9.
    KOiK09458.
    OMAiHAQKRNA.
    PhylomeDBiQ8L3X9.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000447. KAS_II. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    TIGRFAMsiTIGR03150. fabF. 1 hit.
    PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8L3X9-1 [UniParc]FASTAAdd to Basket

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    MATSNLRRHL SASRLRLNRF ISTSSSYHSH RRVVVTGLGM VTPLGRGVET    50
    TWRRLIDGEC GIRGLTLDDL KMKSFDEETK LYTFDQLSSK VAAFVPYGSN 100
    PGEFDEALWL NSKAVANFIG YAVCAADEAL RDAEWLPTEE EEKERTGVSI 150
    GGGIGSICDI VEAAQLICEK RLRRLSPFFI PKILVNMASG HVSMKYGFQG 200
    PNHAAVTACA TGAHSIGDAT RMIQFGDADV MVAGGTESSI DALSVAGFSR 250
    SRALSTKFNS SPQEASRPFD CDRDGFVIGE GSGVIVLEEY EHAKRRGAKI 300
    YAELCGYGMS GDAHHITQPP EDGKGAVLAM TRALRQSGLC PNQIDYVNAH 350
    ATSTPIGDAV EARAIKTVFS EHATSGTLAF SSTKGATGHL LGAAGAVEAI 400
    FSILAIHHGV APMTLNVKNP DPIFDKRFMP LTTSKKMLVR TAMSNSFGFG 450
    GTNASLLFAS I 461
    Length:461
    Mass (Da):49,379
    Last modified:October 1, 2002 - v1
    Checksum:iD9B02B19F5A5FB45
    GO

    Sequence cautioni

    The sequence AAD25826.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073746 mRNA. Translation: BAB91181.1.
    AC006951 Genomic DNA. Translation: AAD25826.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05844.1.
    AY099587 mRNA. Translation: AAM20439.1.
    AY128832 mRNA. Translation: AAM91232.1.
    PIRiF84458.
    RefSeqiNP_178533.2. NM_126485.3.
    UniGeneiAt.41299.

    Genome annotation databases

    EnsemblPlantsiAT2G04540.1; AT2G04540.1; AT2G04540.
    GeneIDi814996.
    KEGGiath:AT2G04540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073746 mRNA. Translation: BAB91181.1 .
    AC006951 Genomic DNA. Translation: AAD25826.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05844.1 .
    AY099587 mRNA. Translation: AAM20439.1 .
    AY128832 mRNA. Translation: AAM91232.1 .
    PIRi F84458.
    RefSeqi NP_178533.2. NM_126485.3.
    UniGenei At.41299.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W0I X-ray 2.10 A/B 31-461 [» ]
    2IX4 X-ray 1.95 A/B 31-461 [» ]
    ProteinModelPortali Q8L3X9.
    SMRi Q8L3X9. Positions 31-461.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G04540.1-P.

    Proteomic databases

    PaxDbi Q8L3X9.
    PRIDEi Q8L3X9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G04540.1 ; AT2G04540.1 ; AT2G04540 .
    GeneIDi 814996.
    KEGGi ath:AT2G04540.

    Organism-specific databases

    TAIRi AT2G04540.

    Phylogenomic databases

    eggNOGi COG0304.
    HOGENOMi HOG000060166.
    InParanoidi Q8L3X9.
    KOi K09458.
    OMAi HAQKRNA.
    PhylomeDBi Q8L3X9.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci ARA:AT2G04540-MONOMER.
    MetaCyc:AT2G04540-MONOMER.
    BRENDAi 2.3.1.41. 399.

    Miscellaneous databases

    EvolutionaryTracei Q8L3X9.
    PROi Q8L3X9.

    Gene expression databases

    Genevestigatori Q8L3X9.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR017568. 3-oxoacyl-ACP_synth-2.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000447. KAS_II. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    TIGRFAMsi TIGR03150. fabF. 1 hit.
    PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and molecular characterization of the beta-ketoacyl-[acyl carrier-protein] synthase component of the Arabidopsis mitochondrial fatty acid synthase."
      Yasuno R., von Wettstein-Knowles P., Wada H.
      J. Biol. Chem. 279:8242-8251(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Mitochondrial protein lipoylation does not exclusively depend on the mtKAS pathway of de novo fatty acid synthesis in Arabidopsis."
      Ewald R., Kolukisaoglu U., Bauwe U., Mikkat S., Bauwe H.
      Plant Physiol. 145:41-48(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Structure of the mitochondrial beta-ketoacyl-[acyl carrier-protein] synthase from Arabidopsis and its role in fatty acid synthesis."
      Olsen J.G., Rasmussen A.V., von Wettstein-Knowles P., Henriksen A.
      FEBS Lett. 577:170-174(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 31-461, SUBUNIT.

    Entry informationi

    Entry nameiKASM_ARATH
    AccessioniPrimary (citable) accession number: Q8L3X9
    Secondary accession number(s): Q9SJB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mitochondrial protein lipoylation in leaves does not exclusively depend on the lipoate biosynthesis by KAS and may occur independently of this pathway in roots.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3