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Protein

Serine/arginine-rich SC35-like splicing factor SCL30

Gene

SCL30

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in intron recognition and spliceosome assembly (Probable). Probably active at the 5' splice sites.Curated

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • nucleotide binding Source: InterPro
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA splicing, via spliceosome Source: TAIR
  • response to oxidative stress Source: TAIR
  • RNA splicing Source: TAIR
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich SC35-like splicing factor SCL30
Short name:
At-SCL30
Short name:
AtSCL30
Alternative name(s):
SC35-like splicing factor 30
Serine/arginine-rich splicing factor 30
Gene namesi
Name:SCL30
Ordered Locus Names:At3g55460
ORF Names:T22E16.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G55460.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: TAIR
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Serine/arginine-rich SC35-like splicing factor SCL30PRO_0000429600Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphoserineCombined sources
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei22 – 221PhosphoserineCombined sources
Modified residuei182 – 1821PhosphoserineBy similarity
Modified residuei204 – 2041PhosphoserineCombined sources
Modified residuei206 – 2061PhosphoserineCombined sources
Modified residuei209 – 2091PhosphotyrosineCombined sources
Modified residuei254 – 2541PhosphoserineBy similarity
Modified residuei256 – 2561PhosphoserineCombined sources
Modified residuei260 – 2601PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8L3X8.
PRIDEiQ8L3X8.

PTM databases

iPTMnetiQ8L3X8.

Expressioni

Gene expression databases

GenevisibleiQ8L3X8. AT.

Interactioni

Subunit structurei

Component of the spliceosome. Interacts with RS2Z33, CYP59, CYP63 and CYP95.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-927061,EBI-927061
CYP63Q9LY754EBI-927061,EBI-2360522
SCL28Q1PDV23EBI-927061,EBI-927052
SCL30AQ9LHP23EBI-927061,EBI-927082
SNRNP35Q8VY742EBI-927061,EBI-927038

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi10028. 21 interactions.
IntActiQ8L3X8. 19 interactions.
STRINGi3702.AT3G55460.1.

Structurei

3D structure databases

ProteinModelPortaliQ8L3X8.
SMRiQ8L3X8. Positions 34-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 12579RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 2823Pro-richAdd
BLAST
Compositional biasi15 – 4733Gly-richAdd
BLAST
Compositional biasi106 – 255150Arg-richAdd
BLAST
Compositional biasi141 – 18242Ser-richAdd
BLAST
Compositional biasi212 – 2165Poly-Ala

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000276233.
KOiK12900.
OMAiNGQIFAG.
OrthoDBiEOG093606WC.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8L3X8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRYSPPYYS PPRRGYGGRG RSPPPPPPRR GYGGGGGGGG RRGSSHGSLL
60 70 80 90 100
VRNIPLDCRP EELREPFERF GPVRDVYIPR DYYSGQPRGF AFVEFVDAYD
110 120 130 140 150
AGEAQRSMNR RSFAGREITV VVASESRKRP EEMRVKTRTR SREPSGSRDR
160 170 180 190 200
SHGRSRSRSI SRSRSPRRPS DSRSRYRSRS YSPAPRRRGG PPRGEEDENY
210 220 230 240 250
SRRSYSPGYE GAAAAAPDRD RNGDNEIREK PGYEAEDRRR GGRAVSRSPS
260
GSRSRSVEVS PR
Length:262
Mass (Da):29,567
Last modified:October 1, 2002 - v1
Checksum:iD9D602E6F42EEDBB
GO

Sequence cautioni

The sequence CAB75904 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141A → S in CAC03602 (PubMed:12176998).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293798 mRNA. Translation: CAC03602.1.
AL132975 Genomic DNA. Translation: CAB75904.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79387.1.
AY099649 mRNA. Translation: AAM20500.1.
AY128836 mRNA. Translation: AAM91236.1.
AY086131 mRNA. Translation: AAM63336.1.
PIRiT47685.
RefSeqiNP_567021.1. NM_115404.5. [Q8L3X8-1]
UniGeneiAt.10035.

Genome annotation databases

EnsemblPlantsiAT3G55460.1; AT3G55460.1; AT3G55460. [Q8L3X8-1]
GeneIDi824712.
KEGGiath:AT3G55460.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293798 mRNA. Translation: CAC03602.1.
AL132975 Genomic DNA. Translation: CAB75904.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79387.1.
AY099649 mRNA. Translation: AAM20500.1.
AY128836 mRNA. Translation: AAM91236.1.
AY086131 mRNA. Translation: AAM63336.1.
PIRiT47685.
RefSeqiNP_567021.1. NM_115404.5. [Q8L3X8-1]
UniGeneiAt.10035.

3D structure databases

ProteinModelPortaliQ8L3X8.
SMRiQ8L3X8. Positions 34-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10028. 21 interactions.
IntActiQ8L3X8. 19 interactions.
STRINGi3702.AT3G55460.1.

PTM databases

iPTMnetiQ8L3X8.

Proteomic databases

PaxDbiQ8L3X8.
PRIDEiQ8L3X8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G55460.1; AT3G55460.1; AT3G55460. [Q8L3X8-1]
GeneIDi824712.
KEGGiath:AT3G55460.

Organism-specific databases

TAIRiAT3G55460.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000276233.
KOiK12900.
OMAiNGQIFAG.
OrthoDBiEOG093606WC.

Miscellaneous databases

PROiQ8L3X8.

Gene expression databases

GenevisibleiQ8L3X8. AT.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRC30_ARATH
AccessioniPrimary (citable) accession number: Q8L3X8
Secondary accession number(s): Q9FYA9, Q9M2T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The splicing pattern of the pre-mRNA is regulated in a tissue-specific manner and by development, and changes in response to various types of abiotic stresses.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.