Q8L3K8 (ASPT_TETHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate/alanine antiporter

Gene names

Name:

aspT

Encoded on

Plasmid pD1

Organism

Tetragenococcus halophilus (Pediococcus halophilus)

Taxonomic identifier

51669 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Tetragenococcus

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the electrogenic exchange of aspartate with alanine. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the AAE transporter (TC 2.A.81) family. [View classification]

Ontologies

Keywords
Biological process	Antiport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Plasmid
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	antiporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-6406530,EBI-6406530

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Aspartate/alanine antiporter		PRO_0000208748
Regions
	Transmembrane	4 – 26	23	Helical; Potential
	Transmembrane	33 – 55	23	Helical; Potential
	Transmembrane	88 – 110	23	Helical; Potential
	Transmembrane	117 – 139	23	Helical; Potential
	Transmembrane	159 – 178	20	Helical; Potential
	Transmembrane	362 – 381	20	Helical; Potential
	Transmembrane	385 – 407	23	Helical; Potential
	Transmembrane	428 – 450	23	Helical; Potential
	Transmembrane	455 – 477	23	Helical; Potential
	Transmembrane	520 – 542	23	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8L3K8 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3A86075EEEA942DA	FASTA	543	57,213
	10 20 30 40 50 60 MNAIGNFLVG TPVFTIFICL ALGYLLGKLK IGSFTLGATV GVLIVALLIG QLGVFPRDTL 70 80 90 100 110 120 LGDIFFDFFM FAIGYRVGPS FISSMKKFGA KIVYATLIFL VSAFIVAYAC FKMFHIGPGI 130 140 150 160 170 180 AAGIIAGGLT QSAVIGSSLE TISKLPISDH LKTLYSNQIP IVYTLTYVFG TIGVLIFLRD 190 200 210 220 230 240 IMPKLMHIDL KKQAVKTAKE LDMIPVPVIV ASTHFYTIND GSSLIGQTLG TVNTKFAKGL 250 260 270 280 290 300 VAAGLNDSAD MASVINAGDV LAISGGIDEI GRAVQEFNLL EVTGKTKAYV SKQVVLKKNF 310 320 330 340 350 360 SADVLKNAQD KGVLVATLAG DVMDPAQFST LKPAESVTLV GQKDAVSEVQ SQLGRLRAAE 370 380 390 400 410 420 NIINYSWFAL GIALSAALGI VGTKVSGVPI ALGGGTASLI VGLVQSIYRD KHAHMDTIPD 430 440 450 460 470 480 SLLEFFQSIG LNLFIATVGL SAAKTFISAI QSMGISVLLI GAVISILPHI ITFVICYYLM 490 500 510 520 530 540 KMEPISIIGA QTGADTLSAA LNDVSERVGS DASPFFAAAV APAYAIGNIF LTLMGPIFIV LLS

References

[1]

"Plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reaction." Abe K., Ohnishi F., Yagi K., Nakajima T., Higuchi T., Sano M., Machida M., Sarker R.I., Maloney P.C. J. Bacteriol. 184:2906-2913(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: D10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB072729 Genomic DNA. Translation: BAB92081.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	2.A.81.1.1. aspartate:alanine exchanger (AAEx) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022457. Asp_Ala_antiprt. IPR006512. YidE_YbjL. [Graphical view]
Pfam	PF06826. Asp-Al_Ex. 2 hits. [Graphical view]
TIGRFAMs	TIGR03802. Asp_Ala_antiprt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPT_TETHA

Accession

Primary (citable) accession number: Q8L3K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program