Q8L2J7 (LEP_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	RT0020

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Complements E.coli mutants temperature-sensitive for lepB function.
Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Signal peptidase I		PRO_0000316278
Regions
	Topological domain	1 – 18	18	Cytoplasmic Potential
	Transmembrane	19 – 39	21	Helical; Potential
	Topological domain	40 – 264	225	Periplasmic Potential
Sites
	Active site	43	1	By similarity
	Active site	106	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8L2J7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: E35E28BD4585A002	FASTA	264	30,849
	10 20 30 40 50 60 MNRDNTKTNK TVKQEFASFT FVICIALVIR ILIMEPFTVP TGSMKATILE NDYIFSTKYS 70 80 90 100 110 120 YGYSNYSLSF FDFIPLFKGR VFAREPERGD IVVFRPPNDM SVRYIKRLIG LPGDKIQLID 130 140 150 160 170 180 DVIYINDKKI ERTEVGTYIG EDGIKYLKFK ETLPNGRTYF SYKLAPIFGI ISNDRYSNTG 190 200 210 220 230 240 VFYVPEGQYF FLGDNRDRSN DSRVNLGFVP FENFIGKAQF IWFSTKITWW DNDIGIINLI 250 260 LKLKPWIESV RLSRIFKNLY NVDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi." Rahman M.S., Simser J.A., Macaluso K.R., Azad A.F. J. Bacteriol. 185:4578-4584(2003) [PubMed: 12867468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION IN E.COLI. Strain: Ethiopian AZ322.
[2]	"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF503336 Genomic DNA. Translation: AAM22228.1. AE017197 Genomic DNA. Translation: AAU03508.1.
RefSeq	YP_066990.1. NC_006142.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ProteinModelPortal	Q8L2J7.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2958484.
GenomeReviews	Gene locus RT0020 in contig AE017197_GR.
KEGG	rty:RT0020.
PATRIC	17909190. VBIRicTyp34752_0018.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG596607.
OMA	KPWIESV.
ProtClustDB	CLSK870751.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0020-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICTY

Accession

Primary (citable) accession number: Q8L2J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program