Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi98ZincUniRule annotation1
Metal bindingi100ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Metal bindingi127ZincUniRule annotation1
Binding sitei251ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_2393-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Synpcc7942_2393
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196771 – 481Glutamate--tRNA ligaseAdd BLAST481

Proteomic databases

PRIDEiQ8L1E5.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi1140.Synpcc7942_2393.

Structurei

3D structure databases

ProteinModelPortaliQ8L1E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionAdd BLAST11
Motifi248 – 252"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252722.
KOiK01885.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8L1E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVRVRIAPS PTGNLHIGTA RTAVFNWLFA RRHQGQFILR IEDTDLERSR
60 70 80 90 100
SEYTDNILTG LQWLGLNWDE GPFYQTQRLD LYKAAVQQLL DSGKAYRCYC
110 120 130 140 150
TEAELEALRE SQRARNEAPR YDNRHRDLTP EQEAAFQAEG REAVIRFRID
160 170 180 190 200
DDREIAWTDL VRDRVVWKGS DLGGDMVIAR RSPAGTIGQP LYNLAVVVDD
210 220 230 240 250
IDMTISHVIR GEDHIANTAK QILLYEALGA AVPEFAHTPL ILNKEGRKLS
260 270 280 290 300
KRDGVTSISD FQNLGYLPEA IANYMTLLGW SPVEGMDERF SLAEAATVFD
310 320 330 340 350
FDRVNKAGAK FDWDKLNWLN SQVIKEKSAS ELVALLQPFW SKAGVDTAAY
360 370 380 390 400
PAAWLEELAT LLGPSLVTLT DIVGQSQLFF SQGIELQEDA IAQLGQAGSK
410 420 430 440 450
AVLQQILEAL PSEALTLEVA KGLIDQAVKA AGVKKGIGMR SLRAALMGSM
460 470 480
QGPDLLTSWV LLHQAGQAQP RLQAAIAAAQ G
Length:481
Mass (Da):53,200
Last modified:October 1, 2002 - v1
Checksum:i82AC99209A06D033
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ440366 Genomic DNA. Translation: CAD29422.1.
CP000100 Genomic DNA. Translation: ABB58423.1.
RefSeqiWP_011244022.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB58423; ABB58423; Synpcc7942_2393.
KEGGisyf:Synpcc7942_2393.
PATRICi23790343. VBISynElo51371_2686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ440366 Genomic DNA. Translation: CAD29422.1.
CP000100 Genomic DNA. Translation: ABB58423.1.
RefSeqiWP_011244022.1. NC_007604.1.

3D structure databases

ProteinModelPortaliQ8L1E5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1140.Synpcc7942_2393.

Proteomic databases

PRIDEiQ8L1E5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB58423; ABB58423; Synpcc7942_2393.
KEGGisyf:Synpcc7942_2393.
PATRICi23790343. VBISynElo51371_2686.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252722.
KOiK01885.
OrthoDBiPOG091H021W.

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_2393-MONOMER.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_SYNE7
AccessioniPrimary (citable) accession number: Q8L1E5
Secondary accession number(s): Q31KJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.