Reviewed, UniProtKB/Swiss-Prot Q8L1D5 (SLDB2_GLUOX)

Names and origin

Protein names

Recommended name:
    Glycerol dehydrogenase small subunit
    EC=1.1.99.22
Alternative name(s):
    Gluconate/polyol dehydrogenase small subunit
    D-sorbitol dehydrogenase subunit sldB
      Short name=SLDH
    D-arabitol dehydrogenase small subunit
      Short name=ARDH

Gene names

Name:

sldB

Organism

Gluconobacter oxydans (Gluconobacter suboxydans)

Taxonomic identifier

442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of glycerol to glycerone. Also acts, more slowly, on a number of other polyols including D-sorbitol, D-arabinitol, D-mannitol, meso-erythritol, adonitol and propylene glycol.
Catalytic activity	Glycerol + acceptor = glycerone + reduced acceptor.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 126	125	Glycerol dehydrogenase small subunit		PRO_0000076320
Regions
	Transmembrane	13 – 33	21	Potential
	Transmembrane	41 – 61	21	Potential
	Transmembrane	67 – 87	21	Potential
	Transmembrane	92 – 112	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8L1D5-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BB4D1492FB83ED86	FASTA	126	13,736
	10 20 30 40 50 60 MPNLQGNRTL TEWLTLLLGV IVLLVGLFFV IGGADLAMLG GSTYYVLCGI LLVASGVFML 70 80 90 100 110 120 MGRTLGAFLY LGALAYTWVW SFWEVGFSPI DLLPRAFGPT ILGILVALTI PVLRRMESRR TLRGAV

References

[1]

"Membrane-bound D-sorbitol dehydrogenase of Gluconobacter suboxydans IFO 3255 -- enzymatic and genetic characterization." Hoshino T., Sugisawa T., Shinjoh M., Tomiyama N., Miyazaki T. Biochim. Biophys. Acta 1647:278-288(2003) [PubMed: 12686146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 3255 / IAM 12306 / LMG 1487.

[2]

"5-keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in gluconobacter species." Matsushita K., Fujii Y., Ano Y., Toyama H., Shinjoh M., Tomiyama N., Miyazaki T., Sugisawa T., Hoshino T., Adachi O. Appl. Environ. Microbiol. 69:1959-1966(2003) [PubMed: 12676670] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION. Strain: IFO 3257 / LMG 1489.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB065091 Genomic DNA. Translation: BAC02908.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13709.
BRENDA	1.1.99.22. 3050.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

SLDB2_GLUOX

Accession

Primary (citable) accession number: Q8L1D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 25 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)