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Q8L1B1 (SYI2_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase 2

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase 2
Short name=IleRS 2
Gene names
Name:ileS2
Synonyms:mupM
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length1030 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003

Confers high-level resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog produced by P.fluorescens NCIMB 10586 itself that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis. HAMAP-Rule MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Cofactor

Zinc By similarity. HAMAP-Rule MF_02003

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02003

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003

Miscellaneous

P.fluorescens NCIMB 10586 possesses two distinct IleRSs (IleRS-R1 and IleRS-R2), each with a different level of sensitivity to mupirocin. Purified IleRs-R2 shows no sensitivity to mupirocin even at a concentration of 5 mM, 100'000 fold higher than the Ki value of IleRS-R1.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=10 µM for isoleucine

KM=330 µM for ATP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 10301029Isoleucine--tRNA ligase 2 HAMAP-Rule MF_02003
PRO_0000098556

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_02003
Motif589 – 5935"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site5921ATP By similarity

Experimental info

Sequence conflict1751G → R in AAL85500. Ref.1
Sequence conflict4001A → T in AAM12927. Ref.2
Sequence conflict6671L → P in AAM12927. Ref.2
Sequence conflict6711L → P in AAM12927. Ref.2
Sequence conflict7171Y → H in AAM12927. Ref.2
Sequence conflict8051R → C in AAM12927. Ref.2
Sequence conflict8861A → V in AAM12927. Ref.2
Sequence conflict9391S → P in AAM12927. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8L1B1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 2D930693BE1DC935

FASTA1,030117,875
        10         20         30         40         50         60 
MSTEGSGPVR FPAMEDAVLE RWEKEKTFEQ SISAREGKPV YVFYDGPPFA TGLPHYGHIL 

        70         80         90        100        110        120 
TSYIKDVIPR YQTMLGKQVP RRWGWDCHGL PVEFEVEKAM GFKSKRDILE FGVEQFNDEC 

       130        140        150        160        170        180 
RELVLKYADD WRGFVNRMGR WVDFDGAYKT MDNDYMESVL WGFKTLHDKG HVYEGGKIVP 

       190        200        210        220        230        240 
YCVRCQTVLS NFEARLDDAF RPRRDMSAYV KFRQQDRPDT FFLAWTTTPW TLPANVALAV 

       250        260        270        280        290        300 
AADENYVCIE HGEERLWLAE GCLGGLFDEP VILERCTGAE LAGLRYLPVV GEVIDASAHR 

       310        320        330        340        350        360 
VVTADFVQMG DGSGIVHIAP AFGEDDALLG QQYELPAPNP VRDDGTFSDA VAQYAGQNIF 

       370        380        390        400        410        420 
EATPRILADL KSSGLLFKQE QIEHNYPHCW RCDNPLIYRA VESWFIRASA LREQLVENNS 

       430        440        450        460        470        480 
QVNWVPEHVK EGRFGDWIRN ARDWAVSRNR FWGAPIPVWR CDQCGTVEVM GSIAQIEARS 

       490        500        510        520        530        540 
GRKVEDLHVP HIDEHRFACQ CCEGTMSRVT GVFDCWFESG AMPFASRHYP FENKQEFEQT 

       550        560        570        580        590        600 
FPADFIVEYL AQTRGWFYTM MVISTGCFEQ NPFKNAMCHG VILAKDGRKM SKRLKNYPNP 

       610        620        630        640        650        660 
MDLMQTHGSD ALRVALLASP VCKGEDIKFS EESVRDVVRR YHLLFWNCLQ FYKTFTEIDQ 

       670        680        690        700        710        720 
FSPSGDLGQP LDNVLDHYLL HELAALESDI KMWMESLDFS KIYSRIEVFI NVLSTWYLRL 

       730        740        750        760        770        780 
NKARIWRDGL DDDKRQCYEV LHYALSNFAR LLAPFMPFLA EAVYTELGYA DSVHLQDWPS 

       790        800        810        820        830        840 
IDRQYLSYEL ADEMSSLRNL IASVRNVRET NGVSQKFPLR SIRVAGIEQA VLERYAQFLE 

       850        860        870        880        890        900 
EELNVKQVQW AADADEWAQP VVVLIFSLLG KRLGPAMKAV TTAVKAGEYV IDEQGGLVAA 

       910        920        930        940        950        960 
GQTIQPHEFE RRLTVRDTLN NVGIVENMVV WLDLDIDASL KREGAVRELN RRLQDLRKKA 

       970        980        990       1000       1010       1020 
KLGYTEKVDI AVLGGAYVDE ILVHHEDWLK SQLLVQSLLR SDLEAPLAVD EVELPEGDPV 

      1030 
RIQLRRSVLA 

« Hide

References

[1]"Analysis of rILERS, an isoleucyl-tRNA synthetase gene associated with mupirocin production by Pseudomonas fluorescens NCIMB 10586."
Rangaswamy V., Hernandez-Guzman G., Shufran K.A., Bender C.L.
DNA Seq. 13:343-351(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49323 / NCIMB 10586.
[2]"Nucleotide sequence of a 75 kb region of the chromosome of Pseudomonas fluorescens NCIMB 10586 required for biosynthesis of the polyketide antibiotic mupirocin."
El-Sayed A.K., Hothersall J., Cooper S.M., Stephens E., Thomas C.M.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49323 / NCIMB 10586.
[3]"How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid? Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense."
Yanagisawa T., Kawakami M.
J. Biol. Chem. 278:25887-25894(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, RESISTANCE TO MUPIROCIN.
Strain: ATCC 49323 / NCIMB 10586.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY079084 Genomic DNA. Translation: AAL85500.1.
AF318063 Genomic DNA. Translation: AAM12927.1.
AB062785 Genomic DNA. Translation: BAC07171.1.

3D structure databases

ProteinModelPortalQ8L1B1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA6.1.1.5. 5121.
SABIO-RKQ8L1B1.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI2_PSEFL
AccessionPrimary (citable) accession number: Q8L1B1
Secondary accession number(s): Q8GM72, Q8RL58
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 62 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries