Reviewed, UniProtKB/Swiss-Prot Q8KZM8 (BIOD_BACNA)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:

bioD

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

86029 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000187947
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KZM8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 44146F6456CC9151	FASTA	231	25,188
	10 20 30 40 50 60 MRGFFVTGTD TEVGKTVISS GLAALLKEHN RDVGVYKPFL SGISRHHPDS DTSLLKDMSQ 70 80 90 100 110 120 TSLSHEDITP FAFKAPLAPY VAGKLEGKTV TMEEVLSHWG RIREKHECFI VEGAGGISVP 130 140 150 160 170 180 LGEDYLVSHV IKALQLPMII VARPHLGTIN HTFLTVKYAE SMGLPIAGII INGISDSPDE 190 200 210 220 230 DEKTNPEMIE RLCGVPILGV TPKLANVTKE TVLHMVKDHI NLSLLMNQMG V

References

[1]	"Genetic analysis of biotin operon in Bacillus subtilis natto OK2." Sasaki M., Kotanagi T., Kurusu Y. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OK2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB088066 Genomic DNA. Translation: BAC03242.1.
3D structure databases
SMR	Q8KZM8. Positions 3-210.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.3.3. 298357.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BACNA

Accession

Primary (citable) accession number: Q8KZM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)