Q8KZM8 (BIOD_BACNA) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 39.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: ATP-dependent dethiobiotin synthetase BioD EC=6.3.3.3 Alternative name(s): DTB synthetase Short name=DTBS Dethiobiotin synthase | ||
| Gene names |
| ||
| Organism | Bacillus subtilis subsp. natto | ||
| Taxonomic identifier | 86029 [NCBI] | ||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 231 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336 |
| Catalytic activity | ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336 |
| Cofactor | Magnesium By similarity. HAMAP MF_00336 |
| Pathway | Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00336. |
| Sequence similarities | Belongs to the dethiobiotin synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Biotin biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Gene Ontology (GO) | |
| Biological process | biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 231 | 231 | ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336 | PRO_0000187947 | |||||
Regions | |||||||||
| Nucleotide binding | 12 – 17 | 6 | ATP By similarity | ||||||
| Nucleotide binding | 112 – 115 | 4 | ATP By similarity | ||||||
| Nucleotide binding | 202 – 204 | 3 | ATP By similarity | ||||||
Sites | |||||||||
| Metal binding | 12 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 16 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 51 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 112 | 1 | Magnesium 2 By similarity | ||||||
| Binding site | 41 | 1 | Substrate By similarity | ||||||
| Binding site | 51 | 1 | ATP By similarity | ||||||
Sequences
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References
| [1] | "Genetic analysis of biotin operon in Bacillus subtilis natto OK2." Sasaki M., Kotanagi T., Kurusu Y. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OK2. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB088066 Genomic DNA. Translation: BAC03242.1. |
3D structure databases | |
| ProteinModelPortal | Q8KZM8. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| HAMAP | MF_00336. BioD. [Tree] |
| InterPro | IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view] |
| Pfam | PF01656. CbiA. 1 hit. [Graphical view] |
| PIRSF | PIRSF006755. DTB_synth. 1 hit. |
| TIGRFAMs | TIGR00347. BioD. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | BIOD_BACNA | ||||||||
| Accession | Primary (citable) accession number: Q8KZM8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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