Q8KZM8 (BIOD_BACNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:

bioD

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

86029 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187947
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	112 – 115	4	ATP By similarity
	Nucleotide binding	202 – 204	3	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	51	1	Magnesium 2 By similarity
	Metal binding	112	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	51	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KZM8 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 44146F6456CC9151	FASTA	231	25,188
	10 20 30 40 50 60 MRGFFVTGTD TEVGKTVISS GLAALLKEHN RDVGVYKPFL SGISRHHPDS DTSLLKDMSQ 70 80 90 100 110 120 TSLSHEDITP FAFKAPLAPY VAGKLEGKTV TMEEVLSHWG RIREKHECFI VEGAGGISVP 130 140 150 160 170 180 LGEDYLVSHV IKALQLPMII VARPHLGTIN HTFLTVKYAE SMGLPIAGII INGISDSPDE 190 200 210 220 230 DEKTNPEMIE RLCGVPILGV TPKLANVTKE TVLHMVKDHI NLSLLMNQMG V

References

[1]	"Genetic analysis of biotin operon in Bacillus subtilis natto OK2." Sasaki M., Kotanagi T., Kurusu Y. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OK2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB088066 Genomic DNA. Translation: BAC03242.1.
3D structure databases
ProteinModelPortal	Q8KZM8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BACNA

Accession

Primary (citable) accession number: Q8KZM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program