Reviewed, UniProtKB/Swiss-Prot Q8KZA0 (CARA_DESVM)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	DvMF_2370

Organism

Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637) [Complete proteome] [HAMAP]

Taxonomic identifier

883 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112273
Regions
	Domain	191 – 376	186	Glutamine amidotransferase type-1
	Region	1 – 187	187	CPSase HAMAP MF_01209
Sites
	Active site	266	1	Nucleophile By similarity
	Active site	349	1	By similarity
	Active site	351	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KZA0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 20D227856DAAE83E	FASTA	376	41,137
	10 20 30 40 50 60 MRAFLALEDG FVLEGRSFTG RGESGGEVIF NTGMTGYQEV LTDPSYAGQM VCMTYPLIGN 70 80 90 100 110 120 YGVTAEDMES GKVHVEAFIV KECCRTPSNW RAIMSLPDYL AQHGVMGIEG IDTRALTRHL 130 140 150 160 170 180 RINGAMRGII STETDDRDEL VRRARALPTM EGQNLVTRVA PATPYRWDGT RPQPVQLAAD 190 200 210 220 230 240 GAYAWPGTGP RLVVYDYGIK WNILRLLTDQ GFDLLVVPPS FTAMQVAASG AEAVFLSNGP 250 260 270 280 290 300 GDPATLTDEV REIRVMTERM PVAGICLGHQ LLGHALGGTT HKLKFGHHGC NHPVKDLVTG 310 320 330 340 350 360 HIEISSQNHG FCVDIESVPD VEITHVNLND GTLEGFAHKT RPILAVQHHP EASPGPTDSR 370 YFFARFRGMV REAVGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequences of fur, rbr, rlp, and prfC of Desulfovibrio vulgaris (Miyazaki F)." Kitamura M., Numata S., Katsura S., Inoue H. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Desulfovibrio vulgaris str. 'Miyazaki F'." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Richardson P. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB090267 Genomic DNA. Translation: BAC10584.1. CP001197 Genomic DNA. Translation: ACL09311.1.
RefSeq	YP_002436779.1.
3D structure databases
SMR	Q8KZA0. Positions 1-373.
ModBase	Search...
Genome annotation databases
GeneID	7174304.
GenomeReviews	Gene locus DvMF_2370 in contig CP001197_GR.
KEGG	dvm:DvMF_2370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_DESVM

Accession

Primary (citable) accession number: Q8KZA0
Secondary accession number(s): B8DIL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)