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Protein

Protein phosphatase PhpP

Gene

phpP

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase able to dephosphorylate StkP-P and a phosphothreonine residue in a phosphopeptide synthetic substrate. PhpP and its cognate protein kinase StkP appear to constitute a functional signaling couple in vivo, PhpP's primary role being probably to control phosphorylation levels of StkP and of its targets. PhpP thus performs an essential control of StkP activity.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit. Other divalent cations, such as Mg2+ or Ca2+, cannot serve as cofactors.1 Publication

Enzyme regulationi

Phosphatase activity is inhibited by NaF but not by okadaic acid.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Manganese 1By similarity1
Metal bindingi36Manganese 2By similarity1
Metal bindingi37Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi192Manganese 2By similarity1
Metal bindingi231Manganese 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase PhpP (EC:3.1.3.16)
Alternative name(s):
PP2C-type phosphatase
Ser/Thr phosphoprotein phosphatase
Short name:
STPP
Gene namesi
Name:phpP
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

Q8KY51:

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Attempts to construct an in-frame deletion of the phpP gene were unsuccessful and an allele encoding inactive D231A PhpP phosphatase is lethal in a wild-type genetic background. This suggests that PhpP is essential.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi192D → A: Loss of phosphatase activity. 1 Publication1
Mutagenesisi231D → A: Loss of phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004181471 – 246Protein phosphatase PhpPAdd BLAST246

Expressioni

Inductioni

The phpP and stkP genes form an operon.

Interactioni

Subunit structurei

Interacts with the kinase domain of StkP.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiQ8KY51. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ8KY51.
SMRiQ8KY51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 240PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST239

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiENOG4108ZF3. Bacteria.
COG0631. LUCA.

Family and domain databases

CDDicd00143. PP2Cc. 1 hit.
Gene3Di3.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015655. PP2C.
IPR036457. PPM-type_dom_sf.
IPR001932. PPM-type_phosphatase_dom.
PANTHERiPTHR13832. PTHR13832. 1 hit.
SMARTiView protein in SMART
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiView protein in PROSITE
PS51746. PPM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KY51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEISLLTDVG QKRTNNQDYV NHYVNRAGRT MIILADGMGG HRAGNIASEM
60 70 80 90 100
AVTDLGVAWV DTQIDTVNEV REWFAHYLEI ENQKIHQLGQ DEAYRGMGTT
110 120 130 140 150
LEVLAIIDNQ AIYAHIGDSR IGLIRGEEYH QLTSDHSLVN ELLKAGQLTP
160 170 180 190 200
EEAEAHPQKN IITQSIGQKD EIQPDFGTVI LESGDYLLLD SDGLTNMISG
210 220 230 240
SEIRDIVTSD IPLADKTETL VRFANNAGGL DNITVALVSM NEEDAE
Length:246
Mass (Da):27,104
Last modified:October 1, 2002 - v1
Checksum:iCDD968CCE6E86E94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285441 Genomic DNA. Translation: AAM47529.1.

Similar proteinsi

Entry informationi

Entry nameiPHPP_STRPN
AccessioniPrimary (citable) accession number: Q8KY51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: October 1, 2002
Last modified: October 25, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families