Q8KY01 (HPPA_RHOPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1

Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase

Gene names

Name:

hppA

Organism

Rhodopseudomonas palustris

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. HAMAP MF_01129
Subunit structure	Homodimer By similarity. HAMAP MF_01129
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217029
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Transmembrane	164 – 184	21	Helical; Potential
	Transmembrane	236 – 256	21	Helical; Potential
	Transmembrane	259 – 279	21	Helical; Potential
	Transmembrane	290 – 310	21	Helical; Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Transmembrane	388 – 408	21	Helical; Potential
	Transmembrane	467 – 487	21	Helical; Potential
	Transmembrane	503 – 523	21	Helical; Potential
	Transmembrane	578 – 598	21	Helical; Potential
	Transmembrane	599 – 619	21	Helical; Potential
	Transmembrane	667 – 687	21	Helical; Potential
Sites
	Site	461	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KY01 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 64C1C3D1C86AF4A4	FASTA	725	73,750
	10 20 30 40 50 60 MTTSLALYLA LGCGLAAVLY GFIQRGWILA QDAGNARMQE IAGAIQQGAA AYLARQYKTI 70 80 90 100 110 120 AIVGAVLAIL IFFFLGGLTA AGFVLGAVLS GACGFIGMNV SVRANVRTAQ AATKGIGPAL 130 140 150 160 170 180 DVAFKGGAIT GMLVVGLGLL GVSLFFWFLS GGIHADPATL KPLLGLAFGS SLISIFARLG 190 200 210 220 230 240 GGIFTKGADV GADLVGKVEA SIPEDDPRNP AVIADNVGDN VGDCAGMAAD LFETYAVTLI 250 260 270 280 290 300 AAMALGALLL PNAALAAAVY PLVLGGVSII ASIIGCAFVK ASPGMKNVMP ALYKGLIVAG 310 320 330 340 350 360 AISLVFFFFV TRMVMPDDAL GAGTQMRLFG ACVVGLVLTA AMVWVTEYYT GTQFKPVQHV 370 380 390 400 410 420 AQASTTGHGT NIIAGLGVSM KSTAWPVIFV CLAIYGAYAL AGLYGIAIAA TSMLSMAGIV 430 440 450 460 470 480 VARDAYGPIT DNAGGIAEMS GLPDSVRDIT DPLDAVGNTT KAVTKGYAIG SAGLAALVLF 490 500 510 520 530 540 ADYTHALEAR GMSLSFDLSD HRVIVGLFIG GLIPYLFASM AMEAVGRAAG SVVVEVRRQF 550 560 570 580 590 600 RDIKGIMEGT AKPEYGTAVD MLTTAAIKEM IVPSLLPVVA PILVGMLLGP AALGGLLMGT 610 620 630 640 650 660 IVTGIFVGIS MCTGGGAWDN AKKLIEEGFT DANGVLHKKG SEAHKAAVTG DTVGDPYKDT 670 680 690 700 710 720 AGPAVNPLIK IINIVALLIV PLLPLASNPV KASHAEPAPM VQPAETAPAM PAAPMAEPSA PAASQ

References

[1]	"H+-PPase from Rhodopseudomonas palustris: cloning, sequencing and characterization." Garcia-Contreras R., Celis H., Romero I. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF325217 Genomic DNA. Translation: AAM76681.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01129. PPase-energized_pump. [Tree]
InterPro	IPR004131. PPase-energised_H-pump. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_RHOPL

Accession

Primary (citable) accession number: Q8KY01

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	October 1, 2002
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program