ID   BACD2_BACSU             Reviewed;         472 AA.
AC   Q8KWT3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Alanine-anticapsin ligase bacD;
DE            EC=6.3.2.28;
DE   AltName: Full=L-amino acid ligase;
DE   AltName: Full=Bacilysin synthetase;
GN   Name=bacD;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A1/3;
RX   PubMed=15609023; DOI=10.1007/s00203-004-0743-8;
RA   Steinborn G., Hajirezaei M.-R., Hofemeister J.;
RT   "bac genes for recombinant bacilysin and anticapsin production in
RT   Bacillus host strains.";
RL   Arch. Microbiol. 183:71-79(2005).
CC   -!- FUNCTION: Catalyzes the formation of alpha-dipeptides from various
CC       L-amino acids in the presence of ATP. Probably catalyzes the
CC       ligation of L-alanine and L-anticapsin to produce the final
CC       bacilysin antibiotic (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + an L-amino acid + an L-amino acid = ADP
CC       + phosphate + L-aminoacyl-L-amino acid.
CC   -!- COFACTOR: Magnesium or manganese (By similarity).
CC   -!- PATHWAY: Antibiotic biosynthesis; bacilysin biosynthesis.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; AF396778; AAM90571.1; -; Genomic_DNA.
DR   BRENDA; 6.3.2.28; 150.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034026; F:L-amino-acid alpha-ligase activity; IEA:EC.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR011761; ATP-grasp.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; ATP-binding; Ligase; Nucleotide-binding.
FT   CHAIN         1    472       Alanine-anticapsin ligase bacD.
FT                                /FTId=PRO_0000064802.
FT   DOMAIN      142    355       ATP-grasp.
FT   NP_BIND     168    235       ATP (By similarity).
SQ   SEQUENCE   472 AA;  52166 MW;  D39D317DD624E5FC CRC64;
     MERKTVLVIA DLGGCPPHMF YKSAAEKYNL VSFIPRPFAI TASHAALIEK YSVAVIKDKD
     YFKSLADFEH PDSIYWAHED HDKPEEEVVE EIVKVAGMFA VDAITTNNEL FIAPMAKACE
     RLGLRGAGVQ AAENARDKNK MRAAFNRAGV KSIKNKRVTT LEDFRAALQE IGTPLILKPT
     YLASSIGVTL IKEMETAEAE FNRVNEYLKS INVPKAVTFE APFIAEEFLQ GEYDDWYETS
     GYSDYISIEG IMADGEYFPV AIHDKTPQIG FTETSHITPS ILDDDAKRKI VEAAKKANEG
     LGLENCATHT EIKLMKNREA GLIESAARFA GWNMIPNIKK VFGVDMAQLL LDVLCFGKEA
     DLPKGLLEQE PCYVADCHLY PQHFKENGQL PETAVDFVIE SIDIPDGVLK GDTEIVSFSA
     AEAGTSVDLR LFEAFNSIAA FELKGSNSGD VAESIKQIQQ QAKLTAKYAL PV
//