ID   BACD_BACAM              Reviewed;         472 AA.
AC   Q8KWS8;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 25.
DE   RecName: Full=Alanine-anticapsin ligase bacD;
DE            EC=6.3.2.28;
DE   AltName: Full=L-amino acid ligase;
DE   AltName: Full=Bacilysin synthetase;
GN   Name=bacD;
OS   Bacillus amyloliquefaciens.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15841;
RX   PubMed=15609023; DOI=10.1007/s00203-004-0743-8;
RA   Steinborn G., Hajirezaei M.-R., Hofemeister J.;
RT   "bac genes for recombinant bacilysin and anticapsin production in
RT   Bacillus host strains.";
RL   Arch. Microbiol. 183:71-79(2005).
CC   -!- FUNCTION: Catalyzes the formation of alpha-dipeptides from various
CC       L-amino acids in the presence of ATP. Probably catalyzes the
CC       ligation of L-alanine and L-anticapsin to produce the final
CC       bacilysin antibiotic (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + an L-amino acid + an L-amino acid = ADP
CC       + phosphate + L-aminoacyl-L-amino acid.
CC   -!- COFACTOR: Magnesium or manganese (By similarity).
CC   -!- PATHWAY: Antibiotic biosynthesis; bacilysin biosynthesis.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; AF396779; AAM90576.1; -; Genomic_DNA.
DR   BRENDA; 6.3.2.28; 1130.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034026; F:L-amino-acid alpha-ligase activity; IEA:EC.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR011761; ATP-grasp.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; ATP-binding; Ligase; Nucleotide-binding.
FT   CHAIN         1    472       Alanine-anticapsin ligase bacD.
FT                                /FTId=PRO_0000064803.
FT   DOMAIN      142    355       ATP-grasp.
FT   NP_BIND     168    235       ATP (By similarity).
SQ   SEQUENCE   472 AA;  52338 MW;  85E4AEE6142428A0 CRC64;
     MERKTVLVIA DLGGCPPHMF YESAAEKYNL VSFIPRPFAI TASHAALIEK YSIAVIKDKD
     YFKSLADFEH PDSIYWAHED HDKPEEEVVE EIVKVADMFA VDAITTNNEL FIAPMAKACK
     RLGLRGAGVQ AAENARDKNK MRAAFNRAGV KSIKNKRVTT LEDFRAALQE IGTPLILKPT
     YLASSIGVTL IKEMETAEAE FNRVNEYLKS INVPKAVTFE APFIAEEFLQ GEYDDWYETS
     GYSDYISIEG IMADGEYFPV AIHDKTPQIG FTETAHITPS ILDDDAKRKI VEAAKKANEG
     LGLENCATHT EIKLMKNREA GLIESAPRFA GWNMIPNIKK VFGVDMAQLL LDVLCFGKEA
     DLPKGLLEQE PCYVADCHLY PQHFKENGQL PETVVDFVIE SIEIPDGVLK GDTELVSFSA
     AEAGTSVDLR LFEAFNSIAA FELKGSNSND VAESIKQIQQ QAKLTAKYAL SV
//