Q8KUH4 (Q8KUH4_ACTPA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Polyketide synthase EMBL AAM54076.1

Gene names

Name:

asmB EMBL AAM54076.1

Organism

Actinosynnema pretiosum subsp. auranticum EMBL AAM54076.1

Taxonomic identifier

42198 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Actinosynnemataceae › Actinosynnema

Protein attributes

Sequence length	3073 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro scavenger receptor activity Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8KUH4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 66DC2DA015727548	FASTA	3,073	313,428
	10 20 30 40 50 60 MTTREQLLDA LRASLRENER LRATGGAAEP VAIVGAACRF PGGVTSPEEL WDLVVGEVDA 70 80 90 100 110 120 AGPFPGDRGW DLADLFDPDP DRVGRSYVRH GAFLRDMAWF EPGFFGISPR EALVIDPQHR 130 140 150 160 170 180 LLLEVAWEAF ERAGIAPDSV RGRDVGVFGG VLSHDHGSRF RPVPPELEGY LGTGSSGSAA 190 200 210 220 230 240 IGRVAYTLGL EGPAITVDTA CSSSLVAIHL AVRALQRGEC SMALAGGVTV MSTPDTFVEF 250 260 270 280 290 300 SRQRALARDG RCKAFSADAD GTAWGEGAGL VLLQPLSAAR RDGRRVLAVV RGSAVNSDGA 310 320 330 340 350 360 SNGLTAPSGP AQQRVIRAAL ADAGLSAADV DLLEAHGTGT VLGDPIEAQA LLETYGRGRA 370 380 390 400 410 420 GAGPLWLGSV KSNIGHTQGA AGVAGVLKVV QALRNGVLPP TLHAATPTTE VDWDAGAVRV 430 440 450 460 470 480 LDRALPWRGG GEPGSALGDA AGGLEGGAAG GRPGGAAGGV AGGVGGGAAA VRLRRGAVSA 490 500 510 520 530 540 FGASGTNAHL ILEEAPVAEA VAEAVAPATT GSPAAPGAVG APGVTAAPGT FAATAAVPSA 550 560 570 580 590 600 PLPAAASDAP VPVPVPAPVP VVVSGATTAG LRAQAARLAG HLRERPALGP EDVARPLLLS 610 620 630 640 650 660 RAQRERRAVV VARDREALLG GLDALAGGEA GRRLVSGAAD VTGRVVLVFP GQGAHWTGVA 670 680 690 700 710 720 ERLWREAPAF ADSMARCADV LRDLAGWELR EVLVDPVALE RVDVLQPVSF AVVVSLAALW 730 740 750 760 770 780 ASVGVRPDAV VGHSQGEVAA AHVAGALTLA EAARIVVLRS ALIARELSGR GAMLTVVADV 790 800 810 820 830 840 ERVTALLAGF EGRVCVAAVN GPASVTVSGE DAAVREFERV LSARRVLRWR LPGVDFAGHS 850 860 870 880 890 900 PQVDALRAEL LAALGDVAAR EPEIPLLSTV TGRPATRLDA EHWYRNLREP VRFADAVTAL 910 920 930 940 950 960 LDRGHRVFVE VSPHPVLTTS VVDLAAPHRT AVVGTLRRDE GGLDRFLLSA GELHVRGVPV 970 980 990 1000 1010 1020 DLARHAGAGT AEVPTTVFQR ERYWLDAPRP SGDARAAGLV PSGHPLLGAL VPLADGAVLT 1030 1040 1050 1060 1070 1080 GRLSLSAQPW LADHRVLGAV VVPGVALVEL AVRAGDEVGC PALEELVVAA PLVVPESGDV 1090 1100 1110 1120 1130 1140 AVRVVVGPRG ADGTRPVDVL SGDQPPVLHA RGVLGPPRAP EPVGGQWPPA GAEPVDLAGL 1150 1160 1170 1180 1190 1200 YPDFAAKGLE YGPHFQRLTR LWRRGDEVFG EVELTGEQRR AAGFALHPAL LECALHVSSF 1210 1220 1230 1240 1250 1260 TGQRVAGDGG AVGLPFSFSR VALHRSGVDA VRVRAARVGD GVVSLDLADG AGRPVARIGE 1270 1280 1290 1300 1310 1320 LAFRRAGGIR SGPPGVLLET AWRPVELPEA PPPADVTTVV GDSVERVLAA LRETLAGDGR 1330 1340 1350 1360 1370 1380 LLVLTGDPGA PIGTAVGGAV WGLVRSAQLE EPGRIVLADL DTAPERAGAV LDRVLASGEP 1390 1400 1410 1420 1430 1440 QVAIRGGAVL VPRLTPVPVV AGPAGLDPAK TVVLTGGTGA LGRLVARHLV TEHGARRLLL 1450 1460 1470 1480 1490 1500 LGRGGPERAR DLVAELTGLG AEVEALACDV ADRGALSTAL AGRALTAVVH LAGALDDGVV 1510 1520 1530 1540 1550 1560 TALTPERLAA AFAPKALAAR HLDELTRDQD LAAFVLFSSV ASVLGSAGQA NYAAANGFLD 1570 1580 1590 1600 1610 1620 GLAHERHALG LPATSIAWGL WDVDSALVGE RGAADRERLA ASGMRPLPAP AALDLLDAAL 1630 1640 1650 1660 1670 1680 ACGRPAVTAL ALARAADRAP AALGEVVASR RAPAPAAPVP GGPAALLALV RAEAAAVLAL 1690 1700 1710 1720 1730 1740 PVAGVDERRA FRDLGVDSLT GVELRNRLAE ATGLRLPATL VFDHPTPVEL AAALLERVGG 1750 1760 1770 1780 1790 1800 TSGPAGGASA PAPASAPGEP IAIVGMACRA PGGVASPEDL WDLVAGGVDA VGPFPADRGW 1810 1820 1830 1840 1850 1860 DLERLVHPDP DHEGSTYVDR AAFLDDPAGF DADFFGISPR EALAMDPQQR LLLEVAWEAF 1870 1880 1890 1900 1910 1920 ERAGIAPDSV RGTDVGVFAG VTNHDYDQVL PRGDDGYRIT GVSGSVVSGR IAYALGLEGP 1930 1940 1950 1960 1970 1980 ALSVDTACSA SLVAIHLAVR ALRGGECSMA LAGGATVMAT PRGFVEFARQ RGLAPDGRCK 1990 2000 2010 2020 2030 2040 PFSASADGTA WSEGAGLVLL ERLSDARRNG RRVLAVVRGS AVNSDGASNG LTAPSGAAQQ 2050 2060 2070 2080 2090 2100 RVIRAALADA GLSTSDVDAV EAHGTGTRLG DPIEARALVA TYGRDRDGEP LWLGSLKSNL 2110 2120 2130 2140 2150 2160 GHMQGAAGIG GVVKTVQALR HGVLPRTLHV DAPTPEVEWD GVALLAEQRA WPEVDRPRRA 2170 2180 2190 2200 2210 2220 AVSSFGVSGT NAHVVLEQAP AERTAAENPG GNAAGRVGDF PGVLPLVLSA RSPAALRAQA 2230 2240 2250 2260 2270 2280 ARLADVLDDL PLRDVASTLA RGRAAHEHRA VLVAADRDAL RAGLRALAGG RPHAAARTGV 2290 2300 2310 2320 2330 2340 RAPGGLAFAF SGQGSQFVGM GRGLHAAFPA FAEVFDRVCA ALDERLGELT GRSVRDVVLD 2350 2360 2370 2380 2390 2400 GPAELLAETV HAQPALFALE VALARLLEGW GVVPDVVLGH SVGELAAAHV AGALSEADAC 2410 2420 2430 2440 2450 2460 ALVAARARAM HGARRGGAML AVSATEEQVA PLLGGGLALA AVNGPGAVVL SGDGDAVERA 2470 2480 2490 2500 2510 2520 AAECAGRGWR TRALRVSHAF HSAHLDGVLG ELAVAARRVE VRPPRVPLLS TLTGAPADVE 2530 2540 2550 2560 2570 2580 LLADPDYWAR QAREAVRFGD GVARLGELGV TRVLEVGPGG ALAALVREQV APGAAVARGL 2590 2600 2610 2620 2630 2640 VDGDEVVGLL SGIGDLFASG APVDWTALLG GPGAVVDLPT YPFQRTRFWP AAQAPGAWCE 2650 2660 2670 2680 2690 2700 VRLERLPAPR GGGSATVLEV GPTASAEELA SVADVLAEHS GPLLVVTGDG AVAGLVRSLP 2710 2720 2730 2740 2750 2760 DRADVVLADV DDDPASVALL PALHGCGEPE LVVRAGEVLA PRLRRVTATG AAPSGPVLVV 2770 2780 2790 2800 2810 2820 GEAARGVVAA CGVREPVVLP ADSGRAELVA ALDDHRPDWV VLAVAPDDAG LALAALLDEL 2830 2840 2850 2860 2870 2880 TRDRPPALFA LCGPAGALGD AARPEDAVAA GVLAALVRRR RGDGLPATLL CAGPGTPPLS 2890 2900 2910 2920 2930 2940 PELGVVVFAR TPEVVLPEPR TAQPEPDAVD LTGPDRDRVL LALVRDHAAA VLGRADGPVD 2950 2960 2970 2980 2990 3000 GRAAFRDLGF DSMTAVELRD RLAAATGLTL PATLLFDHPT AAEVAAVLAG LLDGGDPVTS 3010 3020 3030 3040 3050 3060 GLDRLEAALA DPPADPALRA SAAARLRALL AALSDEGDGR DGAGQDGAAA LRDAGVAELF 3070 EFIDTRLGRA ERA

References

[1]	"The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum." Yu T.W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J., Moss S.J., Leistner E., Floss H.G. Proc. Natl. Acad. Sci. U.S.A. 99:7968-7973(2002) [PubMed: 12060743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 31565 EMBL AAM54076.1.
[2]	Yu T.-W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J., Moss S.J., Leistner E., Floss H.G. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 31565 EMBL AAM54076.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF453501 Genomic DNA. Translation: AAM54076.1.
3D structure databases
HSSP	HSSP built from PDB template 1MLA based on UniProtKB P25715.
ProteinModelPortal	Q8KUH4.
SMR	Q8KUH4. Positions 1758-2630.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR000794. Beta-ketoacyl_synthase. IPR002198. DH_sc/Rdtase_SDR. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR020807. PKS_dehydratase. IPR020806. PKS_PP-bd. IPR006162. PPantetheine_attach_site. IPR001190. Srcr_rcpt. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 4 hits. G3DSA:1.10.1200.10. ACP_like. 2 hits. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.47.10. Thiolase-like_subgr. 5 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 2 hits. PF00106. adh_short. 1 hit. PF00109. ketoacyl-synt. 2 hits. PF02801. Ketoacyl-synt_C. 2 hits. PF00550. PP-binding. 2 hits. [Graphical view]
SMART	SM00827. PKS_AT. 2 hits. SM00826. PKS_DH. 1 hit. SM00822. PKS_KR. 1 hit. SM00825. PKS_KS. 2 hits. SM00823. PKS_PP. 2 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 2 hits. SSF52151. Acyl_Trfase/lysoPlipase. 2 hits. SSF55048. Malonyl_transacylase_ACP-bd. 2 hits. SSF53901. Thiolase-like. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 2 hits. PS00606. B_KETOACYL_SYNTHASE. 2 hits. PS00012. PHOSPHOPANTETHEINE. 2 hits. PS50287. SRCR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8KUH4_ACTPA

Accession

Primary (citable) accession number: Q8KUH4

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)