Q8KUH3 (Q8KUH3_ACTPA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Polyketide synthase EMBL AAM54075.1

Gene names

Name:

asmA EMBL AAM54075.1

Organism

Actinosynnema pretiosum subsp. auranticum EMBL AAM54075.1

Taxonomic identifier

42198 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Actinosynnemataceae › Actinosynnema

Protein attributes

Sequence length	4684 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8KUH3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C36307EEAFEFE059	FASTA	4,684	483,399
	10 20 30 40 50 60 MLRSDLIRPL PELLREHAAE RGDRVAFRDA RRAVTWVDLE LRTRRLAGHL AARRLLPGDR 70 80 90 100 110 120 AVILLGNRVE TVECYLALVR AGAVGVPLNP VSTDAELGAL LDDSGARLVL TDAAHADQLA 130 140 150 160 170 180 GLRATRPALE VVHVDRDYEA LAGVDAPLPA HDALELDDLA WMLYTSGTTG APKGVLSTQR 190 200 210 220 230 240 NCLWSVAACY APVLGLTPDD RVLWPLPLFH SLSHIAGVLA TTAVGATTRV VDGRSPQDVL 250 260 270 280 290 300 AALHEERSTV LAGVPTTYHH LVRAAGERGF LAPDLRVGLV GGAITTAELR AAVEQRFGVP 310 320 330 340 350 360 LIDAYGSTET CGSITVNWPT GPRVEGSSGL PVPGLAVRLV DPDTGLDAPT GAEGEVWVRG 370 380 390 400 410 420 PSVMVGYHNR PEATAAALRD GWYRTGDLAT RDESGFHAVT GRIDDVVVRG GEKVHPAEVE 430 440 450 460 470 480 AVLRAVPGVA DAAVVGRPHD VLGEVPVAFL VPGEGFDPAQ VLAVCRERLS YHKVPEELYQ 490 500 510 520 530 540 IESVPRTASG KITRRVLLDT PARLRASGER GALFRTDWVP LAAAPGGATA PGAVAGSGAA 550 560 570 580 590 600 TASSAVTGAG AATGPARATT PAAATAPATT PAEASTPVAA ARPGGHPVAV MAEPTAPAPG 610 620 630 640 650 660 GAPVVLDLRG DDAPALSGRP DLVLTRGAVA VDGGPVRLGQ LAAWTAACRA GITAVDADDD 670 680 690 700 710 720 GAVDPVGLLA AGEPRLLVRS GVVLVPRLHE VPGSVVGRGA LDPRRAVAVT GADSGTAAAL 730 740 750 760 770 780 ARHLRGAHGV RDLLLITAPH GRARAGALRD DLVASGARVV LAVAGPDALD RALSRAPSPL 790 800 810 820 830 840 GALVHTGDGD PEALPRLLGA RVELVVCATA FDSAAAEDVV LRRRAAGMPA RCLAWGPWEA 850 860 870 880 890 900 ETAQARPGLA VLPLRDGLAA FDAALGADEP VVSVLRPDPD LVAGDVPAPL RDLLHVVAAE 910 920 930 940 950 960 PEPVRVAELR ASLAGRGERE RRRRLLDLVR AETAALRPAE FLVDGHERGF AELGFTSATS 970 980 990 1000 1010 1020 VALRNRLTAA TGLALPATAA FEHPTPTALA ALLHDELFGA SRTPVAPARA VERPVERSGE 1030 1040 1050 1060 1070 1080 PIAVVAAGCR LPGGVASPED LWEVVRSGAD VVSPFPVDRG WDLDALRGAC AGGGFLADAA 1090 1100 1110 1120 1130 1140 LFDAALFGVH PREALAMDPQ QRLLLEVAWE VFERAGIDPR SVAGDGTGVF AGLMGHDYGS 1150 1160 1170 1180 1190 1200 RPGLDRQLVE GYLSTGNAGS VASGRIAYAF GLTGPALTVD TACSSSLVAV HLAVQSLRRG 1210 1220 1230 1240 1250 1260 ECSMALAGGA TVMATPADFV EFARQGALAP DGRCKPFSAS ADGTAWSEGA GLVLLERLSD 1270 1280 1290 1300 1310 1320 ARRNGRRVLA VIRGSAVNSD GASNGLTAPS GAAQQRVIRA ALADAGLSTS DVDAVEAHGT 1330 1340 1350 1360 1370 1380 GTRLGDPIEA RALVATYGRD RDGEPLWLGS LKSNFGHPQG AAGIAGLIKL VQALRHGVLP 1390 1400 1410 1420 1430 1440 RTLHVDAPTP EVDWDGVEVL AEQRAWPEVD RPRRAAVSSF GVSGTNAHVV LEQAPAETAA 1450 1460 1470 1480 1490 1500 PAAPERGPVP LPVSAVDAAA LRAQARRLAD FLAPSDVTAA ELAGALRAGR ADLGERAVVV 1510 1520 1530 1540 1550 1560 AADRAGALAA LEALAADEPS PALVTGRADV TGRTVLVFPG QGGQWVGMGA RLLDESPVFA 1570 1580 1590 1600 1610 1620 ERVGECAAAV DPLVDFRVVD VLRGGDLSRV DVVQPVAFVV MVALARLWRS VGVEPDAVVG 1630 1640 1650 1660 1670 1680 HSQGEIAAAC VAGALSLADA ARVVVLRSRA IAAGLNGGGM ASIPLPQAQV EELVAGTGVC 1690 1700 1710 1720 1730 1740 VAAVNGPGSV VVSGEVDALV ARVEGARRLP VDYASHSPAV DVLREGLLAD LAPITPRPAA 1750 1760 1770 1780 1790 1800 IPMLSTVTGK WLDGAELDAD YWFANLRHPV RFADATALLV ADGFRAFVEV GAHPVLTAPV 1810 1820 1830 1840 1850 1860 QDLLAGAGPS VATGTLRRDD GGLDRFLRSA AELHVRGVPV DLSPLAPPPV RALDLPTYPF 1870 1880 1890 1900 1910 1920 QRARYWLDAD PTAASALRHP LLTSATDLAG GEDLVCQARL STARHPWLAD HAVRGSAVLP 1930 1940 1950 1960 1970 1980 GTCWAELAIR AGDEVGAPAL DELLVEAPLV LPERGAVEVQ LRIGAPDAAG RRPLEAHARA 1990 2000 2010 2020 2030 2040 EGGAWTRHAS GSLAADRSTP VPLTEWPPTG AREVDLAGFY DSRAAEGYDY GPALRGLRRA 2050 2060 2070 2080 2090 2100 WTRDGEVFAE VELPAPLSPD GWGLHPALFD AALHATGLLP PARVAADEVL LPFSWRGTRL 2110 2120 2130 2140 2150 2160 RATGATALRV RLTGTADDHV LRAYDPEGAP VVEVDALRLR PAPATALVGS GALFRVEWVD 2170 2180 2190 2200 2210 2220 LDLPVSTPDS TPGAPVLDRP VLDLPGGDAR TLVGLTLEAL REHDELTVVT RGAAEDPAAA 2230 2240 2250 2260 2270 2280 AVWGLLRTAQ TERPGRFAVV DLDDDPASRA LLPALPATGE PQVRVRAGRA SAPRLARTRD 2290 2300 2310 2320 2330 2340 DGLPLPAAPT WRLTCDGGTF TDLAATPSPA ANRPLEPGEV RVGVRAAGVN FRDAAVVLNA 2350 2360 2370 2380 2390 2400 VPGQRGLGGE AAGVVLETGP GVTGLAVGDR GLGTFDRDFG AFGPVAVTDA RLLAPVPPGW 2410 2420 2430 2440 2450 2460 GFARAASVPV AFLTALYGLR DLAQVGPGDR VLVHAGAGGV GGAAVRLAAL LGAEVFATAS 2470 2480 2490 2500 2510 2520 PAKWPVLRAL GLDDAHLASS RDTGFAERFP NVDVVLNSLT GELVDASLRL LAPGGRFLEL 2530 2540 2550 2560 2570 2580 GKVDVRDLVR ADVTYRAYDL RDAGPDRTAE LLAEVLGLFA AGRLEPLPLT VRDVRHAPEA 2590 2600 2610 2620 2630 2640 LRHVASGGHV GKVVLTVPRP LDPEGTVLVH GGGALGALTA RHLVSAHGAR HLLITSRRGP 2650 2660 2670 2680 2690 2700 DAPGAAELAA DLAELGATTR FERCDAADRD RVAALLADLG GRPLTAVVHT AGALDDALLA 2710 2720 2730 2740 2750 2760 DQDAARVDAV LRPKLDGALL LDELTRDADL AAFVLFSSAA GTLGNAGQAN YAAANAALDA 2770 2780 2790 2800 2810 2820 LAARRRSAGL PATSIAWGHW EHTSALTAGL TAADLARGAR HGAGSLTTTE ALALLDAALA 2830 2840 2850 2860 2870 2880 SPEPAVLAAA LDPRRAAEVP PLLRDLAGPR RAVAAQAVAG ADRPLIDVVR AHTATVLGHR 2890 2900 2910 2920 2930 2940 DASAVDANRA FKELGVDSLT AVELRNRLAA ATGLALAATS VFDHPTPALL TAHLLAELGH 2950 2960 2970 2980 2990 3000 APAAGTTAPP ATAAVTADAG EPIAIVSMAC RFPGDVSSPE DLWDLVAGGG DALTPFPADR 3010 3020 3030 3040 3050 3060 GWDLAALFAP DPDRAGRSHA REGGFLADVA GFDADLFGIS PREAIGTDPQ QRLLLEVAWE 3070 3080 3090 3100 3110 3120 LFERAGIAPD SLRGTDTGVF AGVIAHGYPV LPAHSDQGLE GHRVTGVSGS VVSGRVAYAF 3130 3140 3150 3160 3170 3180 GLQGPAMTVD TACSSSLVGV HLAVRALRGG ECSLALAGGV TVLSTPDLFV DFSRQRGLAA 3190 3200 3210 3220 3230 3240 DGRCKAFGAG ADGTGFGEGV GLVLLERLWD ARRNGRRVLG LVRGSAVNSD GASNGLTAPS 3250 3260 3270 3280 3290 3300 GPAQERVIGR ALADAGLSAA DVDLVEAHGT GTRLGDPIEA RALVATYGRD RGGEPVWLGS 3310 3320 3330 3340 3350 3360 VKSNIGHTQA AAGVAGLIKA VQALRHGVLP ATLHAAEPTP EVEWDGVAVL AEQRDWPEVG 3370 3380 3390 3400 3410 3420 RPRRAAVSSF GVSGTNAHVV LEQAPEELSP EGRIPEVRIP DRRTSEGRVP EGRAPEGRTS 3430 3440 3450 3460 3470 3480 GGRAPEGQTS EGRAFGGRVP EDQTSEDRTS EGQASMGRAP EGQAPLGQVS ESRAPDHRVP 3490 3500 3510 3520 3530 3540 AGPDRDVPGH LPLVLSAHDP AALRATAARL LPVAERVPLP DLALSLVTGR AALPCRAVIP 3550 3560 3570 3580 3590 3600 ARDRGQALAA LRDLASGATA PAERDGGGVA FLLSGQGSQR AGTGRLLERR FPVFRDALRE 3610 3620 3630 3640 3650 3660 VCALLDRRVV GGPGVRAALD DPGLLADTRY AQAGLFAVQV ALVRLLDALG VRPDLLAGHS 3670 3680 3690 3700 3710 3720 VGEIAVAHAA GVLSLEDAST LVAARGALMR ELPPGVMVAV RAGEDEARAA LVDGVELAAV 3730 3740 3750 3760 3770 3780 NGPRATVLTG DEAAVTEVAA RLGRATRLRV AHAFHSAAVD AVLPAFADVL ARLDFRPPRV 3790 3800 3810 3820 3830 3840 PVATTAPGAL DAPEYWLGQA REPVRFADAV TDLHRRGATA FVEVGPGGGL AAAAQECLDG 3850 3860 3870 3880 3890 3900 HDVLCAPLLH RDRDEDHSLI TALGALHARG ARVDFAPLLA DAGGRTTDLP TYPFQRRRFW 3910 3920 3930 3940 3950 3960 LDPAPPTTDA APLEHPLLTG ELEIPGSDTA AFTGRAATGA HPWLADHRLL DAVVVPGTAL 3970 3980 3990 4000 4010 4020 LDLVRAAGRQ LGLPEVAELV NEVPLVLGDG AALLRVTAAA PGPDGRREVA VHSRPESAGP 4030 4040 4050 4060 4070 4080 RSPWTRHVTG QLAPESGPLA FDLAQWPPPG ALPLPVADHR AAMAATGHGY GPAFLGLRAA 4090 4100 4110 4120 4130 4140 WKLDDEVLVE AVLPDEVADD GHAVHPALLD AVLQAIALTG VEDGSQVRVP FSWRSARGAG 4150 4160 4170 4180 4190 4200 RAGRAVRASL RPDGDGGVRV RVACPRGRPL LEIASLVSRP VTPDRLRPGV LLRQAWTEVG 4210 4220 4230 4240 4250 4260 PPPGAAEPVP LREVGERVPP VVRVDAGHVR DLLPLLQEVV TEPRWESTRL LVTTSGAVGP 4270 4280 4290 4300 4310 4320 RAGRAEGAAV WGLVRSAAAE HPGRFVLADL DDRPESAALL PALATGALPE AVLRAGRVLV 4330 4340 4350 4360 4370 4380 PGLHRVSAPG AGGAVLDPGG AALITGGSGA LAALLAEHLV RAHGVRCLVL LSRSGRGPDV 4390 4400 4410 4420 4430 4440 PGADVRHVRG DVANRADVVA ALAAVPPDRP LTLVAHLAGV LDDGALTALT PERLDAVLRP 4450 4460 4470 4480 4490 4500 KAEGARHLDE LTRDRDLAAF VLFSSVAGAL GHAGQANYAA ANAHLDGLAH ERHALGLPAT 4510 4520 4530 4540 4550 4560 ALAWGPWTEG MARGRGTLSP QVALGLFDAA LRTGLPALVP LDREPERRVP APRAATPAPA 4570 4580 4590 4600 4610 4620 GPSLADRLRP MPPAERRAHL LELVTAEALG VLGHDDGAAL PAGQAFRDAG FDSITAMALR 4630 4640 4650 4660 4670 4680 NRLVRLAGVR LPASVVFDHP TPAATAERLE AELFPADRLP GPRHGDDFDD MAADELVRRA LGGE

References

[1]	"The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum." Yu T.W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J., Moss S.J., Leistner E., Floss H.G. Proc. Natl. Acad. Sci. U.S.A. 99:7968-7973(2002) [PubMed: 12060743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 31565 EMBL AAM54075.1.
[2]	Yu T.-W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J., Moss S.J., Leistner E., Floss H.G. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 31565 EMBL AAM54075.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF453501 Genomic DNA. Translation: AAM54075.1.
3D structure databases
HSSP	HSSP built from PDB template 1PQW based on UniProtKB P96202.
ProteinModelPortal	Q8KUH3.
SMR	Q8KUH3. Positions 1013-1868, 2865-2939, 2961-3416.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR000794. Beta-ketoacyl_synthase. IPR002198. DH_sc/Rdtase_SDR. IPR011032. GroES-like. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR020807. PKS_dehydratase. IPR020843. PKS_ER. IPR020806. PKS_PP-bd. IPR006162. PPantetheine_attach_site. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 4 hits. G3DSA:1.10.1200.10. ACP_like. 3 hits. G3DSA:3.40.50.720. NAD(P)-bd. 3 hits. G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 2 hits. PF08240. ADH_N. 1 hit. PF00106. adh_short. 2 hits. PF00107. ADH_zinc_N. 1 hit. PF00501. AMP-binding. 1 hit. PF00109. ketoacyl-synt. 2 hits. PF02801. Ketoacyl-synt_C. 2 hits. PF00550. PP-binding. 3 hits. [Graphical view]
SMART	SM00827. PKS_AT. 2 hits. SM00826. PKS_DH. 2 hits. SM00829. PKS_ER. 1 hit. SM00822. PKS_KR. 2 hits. SM00825. PKS_KS. 2 hits. SM00823. PKS_PP. 3 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 3 hits. SSF52151. Acyl_Trfase/lysoPlipase. 2 hits. SSF50129. GroES_like. 1 hit. SSF55048. Malonyl_transacylase_ACP-bd. 2 hits. SSF53901. Thiolase-like. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 3 hits. PS00455. AMP_BINDING. 1 hit. PS00606. B_KETOACYL_SYNTHASE. 2 hits. PS00012. PHOSPHOPANTETHEINE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8KUH3_ACTPA

Accession

Primary (citable) accession number: Q8KUH3

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)