Q8KUF6 (Q8KUF6_ACTPA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Polyketide synthase EMBL AAM54078.1

Gene names

Name:

asmD EMBL AAM54078.1

Organism

Actinosynnema pretiosum subsp. auranticum EMBL AAM54078.1

Taxonomic identifier

42198 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Actinosynnemataceae › Actinosynnema

Protein attributes

Sequence length	3324 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8KUF6 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 90FCD88E5F08FB7A	FASTA	3,324	339,527
	10 20 30 40 50 60 MSDEKKLRDY LKRALAENER VQQRLRALET AHREPIAVVA TACRFPGGVA SPEDLWDLVA 70 80 90 100 110 120 RGGDVISGFP ADRGWDLDAL FDGDPEAQGR SYVREGGFLH DATEFDAAFF GISPREALAM 130 140 150 160 170 180 DPQQRVLLET AWEVVERAGI DPTSLRGSRT GVFTGVVYYD FATRLHPVPE GMEGHLGTGI 190 200 210 220 230 240 APSVISGRVA YVLGLHGPAV TLDTGCSSSL VGLHLAAQAL RSGECSLALA GGVTVMSLPG 250 260 270 280 290 300 AFPEFSRQRG LAPDGRCKSF ADAADGTAWS EGVGLVLLER LSDAERAGHP VLAVLRGSAV 310 320 330 340 350 360 NSDGASNGLT APNGVSQQRV IRSALEVAGL RPSDVDLVEA HGTGTRLGDP IEAQAILATY 370 380 390 400 410 420 GQDRAEPVLL GSVKSNLGHT QGAAGAAGLI KVVEALRRGE VPRTLHVDRP SAQVDWSAGN 430 440 450 460 470 480 AALATEHVPW PDRGRPRRAA VSAFGVSGTN AHVVLEGVPA PDLPPRDDRA LPLPLSARAE 490 500 510 520 530 540 PALRALAGRV GALLDGPVAL ADVAGSLADG RARLTHRAVV VAADRAGART ALDALAAGEP 550 560 570 580 590 600 HPDLVTGVAD VDGRTAFVFP GQGTAWVGMG ARLLTESAVF ADSVDRCAAA LAPHTDVDLR 610 620 630 640 650 660 AALTGGPLDR VETVQVAAFA IAVSLAGLWR AHGVEPDAVL GHSQGEIAAA CVAGALTLED 670 680 690 700 710 720 AALVVALRSR AVAEHLSGRG AMLSVALPPH EVESFGLAVA AVNGPESVVL SGPVADVEAV 730 740 750 760 770 780 LAALTATGVR ARRVPVDYAS HSPGVDVLRE VLATALAPVR PRRAHVPFLS STTGAWLDGP 790 800 810 820 830 840 ELDAGYWFAN LRGAVGFHAA TTTLLDAGHR AFVEVSAHPV LTGAVQDSAD GRALVTGTLR 850 860 870 880 890 900 RDHGGLHRFR LSAAELHARG VPVDWNAGSG DRWRRVDLPT TPFHRQRCWP DARRAATDQP 910 920 930 940 950 960 GLRAANHPLL DTVVVLAGDD GVVCTGSAST RAHPWLADHA VSGTALLPGA CWVELAVRAG 970 980 990 1000 1010 1020 DEVGLPVLDE LVVEQPLPLG PRTTPLQVRV GPPDATGRRP VELHSGEGGD WTRHARGHLA 1030 1040 1050 1060 1070 1080 AAAPVTAEDP AEWPPPGARE VDLTGFYERQ ATTGYGYGPA FRGLRRCWTR DGEVFAEVVL 1090 1100 1110 1120 1130 1140 PGEPGAFGVH PALLDAALHA AAVTAPEGVP VLLPFTWTGV ALHATGARAL RVRLTTPRAG 1150 1160 1170 1180 1190 1200 ELALTASDGD GRPVVTVDSL VLRPAGDLTA TRAPRSLFEV VWTETPALTP AATAPRPVLR 1210 1220 1230 1240 1250 1260 HDVPAASAED AVCSTLAVVQ GFLADPRHAD SVLLVVTRGG EGPDGATAAT SAVHGLVRSA 1270 1280 1290 1300 1310 1320 QAEEPGRIVL VDTDGSAPDD LITALAAGPE PQLAVRAGRV TAPRLRRAAP EGAAPVLDPE 1330 1340 1350 1360 1370 1380 GTVLVTGGTG VLGAAVARHL VTARGARHLL LLSRRGPDAP GAADLVAELT GAGARVVVRA 1390 1400 1410 1420 1430 1440 VDVADRAALA AALDAVPAAH PLTGVVHLAA ALDDGTITAL TPDRVRRVLA PKATAAAHLH 1450 1460 1470 1480 1490 1500 ELTSDLALFA LFSSASGVLG APGQGNYAAA NAYLDGLARE RHAAGLPATS IAWGLWAEAT 1510 1520 1530 1540 1550 1560 GLTGSLSGTD RERMAASGVR PLTEAEGLAL FDAALAGPAF VAAGLAGTAT GQVPPVLRDV 1570 1580 1590 1600 1610 1620 VRAARPAAAA VRPARTPADL LALVRAESAS VLGHPAGEPV DADRAFKEAG FDSLTAVELR 1630 1640 1650 1660 1670 1680 NRLTAATGVR LPATLVFDHP TPAALAAHLV ERMGAPPAPR PERAARAASG EPIAIVSIGC 1690 1700 1710 1720 1730 1740 RLPGGVTSPD GLWRLLLDGV DATTGPPDDR GWDPATPYRG GFLDGATAFD AGLFGIGPRE 1750 1760 1770 1780 1790 1800 ALAMDPQQRL LLETSWELFE RAGIDPTSLR GTDTGVFAGV MNQDYLSRAA GAPPDAEGFL 1810 1820 1830 1840 1850 1860 STGNSGSVVS GRLAYTYGLE GPALTVDTAC SSSLVAVHLA VRSLRSGECS LALAGGVTVM 1870 1880 1890 1900 1910 1920 ATTGLFADFD RQGGLAADGR CKAFSADADG TAFGEGAGLV LLERLSDARR NGHAVLAVLR 1930 1940 1950 1960 1970 1980 GTAVNSDGAS NGLTAPNGPA QQRVIRAALA DAGLSPAEVD VVEAHGTGTR LGDPIEAQAV 1990 2000 2010 2020 2030 2040 LATYGRDRGE PVLLGSVKSN LGHTQGAAGV TGLIKLVLAL RHGVVPATLH AGRASAEVNW 2050 2060 2070 2080 2090 2100 EAGAAELVTG TRPWPRTGRP RRAAVSSFGI SGTNAHAVLE QAPPGPEAAP VGGPRAGAAS 2110 2120 2130 2140 2150 2160 AGSGAVGARG AEPVGVARAA GAREPEAGGA VDPGGVCPAP AVGAATRAPD PEAAAPGVPG 2170 2180 2190 2200 2210 2220 PAARPVGAGP DGSARTVDVV GASGPLVPLV VSARDERALA AQVERITALP HDPVDVGWSL 2230 2240 2250 2260 2270 2280 VTTRATLRHR AVLLDGEVVA SGVPAPGGTA WLFTGQGGYR PGTGRALRAR FPAFRDAFDA 2290 2300 2310 2320 2330 2340 TCAELDRALD GRAEHPVRAV ALGERPGLDG TLHAQTAAFA LQTALAAQLG AFGLAPDAVA 2350 2360 2370 2380 2390 2400 GHSVGEIAAA HVAGAFPLDA ACALVAERGV LMRDLRPGAM VAVEVTEEEA LDAIGDLPVA 2410 2420 2430 2440 2450 2460 VAVVNGPEAV VLSGDAEAVV AVAARLGGRR KRLPVDRAFH SPHVDPVLDA LRGLATGTPT 2470 2480 2490 2500 2510 2520 TPLISTTTGR TLTHVGHDHW ADHARGAVRF ADALRALAGS GVTTAVELGP DAVLTPHAAE 2530 2540 2550 2560 2570 2580 VLARAIPALR ADHDEVVALL TALAVLHARG AAVDWRPAFE GAGARRVDLP TYPFQRTRYW 2590 2600 2610 2620 2630 2640 LDAAPRATAT GHPLVDALDV LPGDEVLLSA RWHAGRAGLG ATPLAALLEP VIRAGDEVGC 2650 2660 2670 2680 2690 2700 PVVAELDLAT PVPLDAGPRH VRVRVTAPDA DGRRAVTVHS RPDDEPAWTG HATGVLAPAG 2710 2720 2730 2740 2750 2760 HPEPLPHDSE ALAVGTNGAW PPAGADVPAA GANGASLPHG AGTPAAATNG ARSALPAAGP 2770 2780 2790 2800 2810 2820 APGAELVLPD GAAPDGFGLH PALWEALLPH PARCADVVLH ATGATALRVR GTSPLLAVDG 2830 2840 2850 2860 2870 2880 AGDPVVSVGG VTRVAASPRA VTRGSLFEVV EVPLPAPAPP GPSTALSADL STALSADLRG 2890 2900 2910 2920 2930 2940 VTPAEALDVL QRFAASDAPR LVVRTDRPDD PWQCAVRGLV RSAQAEEPGR ILLVVQDGDG 2950 2960 2970 2980 2990 3000 PEPPPTTREP EVVVRDGALR VPRLRRTAPR HDGVRALPDT VLITGGTGAL GSTLARHLAA 3010 3020 3030 3040 3050 3060 GGVRTLVLAS RRGPAAPGAP ELAADLAGLG ARAHLVAADL TDRAALAALL AEHRPGGVVH 3070 3080 3090 3100 3110 3120 TAGVLDDGVL SALTPARLDA VLAPKALVAA HLDELTRDGT TTLFVLFSSA AGVLGTPGQA 3130 3140 3150 3160 3170 3180 NYAAANAFLD GLAARRRAAG LHALSLAWGP WRSGMAAGVD ASRAGLHPLD DAEATALFDA 3190 3200 3210 3220 3230 3240 ALADGRPLLA PLSLAATPQD ADVPPLLRQL VPVRRRSARA EVAPDPVDRL RGAADHELPE 3250 3260 3270 3280 3290 3300 LLLTLVRAAT ATVLGHRGPE SVDPDEPFWD TGFTSLTAVE LRNLLAERTG VRVTAAAVYE 3310 3320 EPTPRALSAH LRAALRAPHT SGTV

References

[1]	"The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum." Yu T.W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J., Moss S.J., Leistner E., Floss H.G. Proc. Natl. Acad. Sci. U.S.A. 99:7968-7973(2002) [PubMed: 12060743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 31565 EMBL AAM54078.1.
[2]	Yu T.-W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J., Moss S.J., Leistner E., Floss H.G. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 31565 EMBL AAM54078.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF453501 Genomic DNA. Translation: AAM54078.1.
3D structure databases
HSSP	HSSP built from PDB template 1NM2 based on UniProtKB P72391.
ProteinModelPortal	Q8KUF6.
SMR	Q8KUF6. Positions 4-891, 1671-2083.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR000794. Beta-ketoacyl_synthase. IPR002198. DH_sc/Rdtase_SDR. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR020807. PKS_dehydratase. IPR020806. PKS_PP-bd. IPR015083. Polyketide_synth_docking. IPR006162. PPantetheine_attach_site. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 4 hits. G3DSA:1.10.1200.10. ACP_like. 2 hits. G3DSA:3.40.50.720. NAD(P)-bd. 3 hits. G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 2 hits. PF00106. adh_short. 2 hits. PF08990. Docking. 1 hit. PF00109. ketoacyl-synt. 2 hits. PF02801. Ketoacyl-synt_C. 2 hits. PF00550. PP-binding. 2 hits. [Graphical view]
SMART	SM00827. PKS_AT. 2 hits. SM00826. PKS_DH. 2 hits. SM00822. PKS_KR. 2 hits. SM00825. PKS_KS. 2 hits. SM00823. PKS_PP. 2 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 2 hits. SSF52151. Acyl_Trfase/lysoPlipase. 2 hits. SSF55048. Malonyl_transacylase_ACP-bd. 2 hits. SSF53901. Thiolase-like. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 2 hits. PS00606. B_KETOACYL_SYNTHASE. 2 hits. PS00012. PHOSPHOPANTETHEINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8KUF6_ACTPA

Accession

Primary (citable) accession number: Q8KUF6

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)