Q8KTE1 (FUMC_METEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Synonyms:	fumA
Ordered Locus Names:	MexAM1_META1p2857

Organism

Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) [Complete proteome] [HAMAP]

Taxonomic identifier

272630 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161287
Regions
	Region	136 – 139	4	B site By similarity
	Region	146 – 148	3	Substrate binding By similarity
Sites
	Binding site	107	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KTE1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6B24EDD6A27775E7	FASTA	472	49,739
	10 20 30 40 50 60 MSPHENPSVE TRTESDTFGP IEVPAHRYWG AQTQRSIQNF KIGTERQPAP LVHALGIVKQ 70 80 90 100 110 120 AAALVNKDLG GLDPKIADAI AESAAEVVAG KHDDEFPLVV WQTGSGTQSN MNANEVIASL 130 140 150 160 170 180 ANERLGGKRG GKSPVHPNDH CNRGQSSNDT FPTAMHIAVA REVQERLLPA LSHLHTALDA 190 200 210 220 230 240 KAKEFESIVK IGRTHLQDAT PVSLGQEFSG YAAQVALGGA RIAATLPGVL ALAQGGTAVG 250 260 270 280 290 300 TGLNAHPEFA ERFAAKVAEL TGLPFTSAEN KFEALATHDA LVFLQGALTA LASGLFKIAN 310 320 330 340 350 360 DIRLLGSGPR SGLGELSLPE NEPGSSIMPG KVNPTQCEAL TMVCAQVVGN GTTVSFAGSQ 370 380 390 400 410 420 GHFELNVFKP VIANAVLQSV RILADASVSF TDNCVVGIKA NTDRISDLMS RSLMLVTALA 430 440 450 460 470 PSIGYDKAAE IAKTAHKNGT TLKEEALRLG YVTDEEFERV VRPETMLAPS AE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstruction of C3 and C4 metabolism in Methylobacterium extorquens AM1 using transposon mutagenesis." Van Dien S.J., Okubo Y., Hough M.T., Taitano T., Lidstrom M.E. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed: 19440302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14718 / DSM 1338 / AM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF497854 Genomic DNA. Translation: AAN03819.1. CP001510 Genomic DNA. Translation: ACS40610.1.
RefSeq	YP_002963887.1. NC_012808.1.
3D structure databases
ProteinModelPortal	Q8KTE1.
SMR	Q8KTE1. Positions 12-469.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8KTE1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7992744.
PATRIC	22511447. VBIMetExt101010_2790.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RIEKDTM.
ProtClustDB	CLSK2331283.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. FumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_METEA

Accession

Primary (citable) accession number: Q8KTE1
Secondary accession number(s): C5AUK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program