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Protein

Elongation factor Tu

Gene

tuf

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTPUniRule annotation8
Nucleotide bindingi81 – 85GTPUniRule annotation5
Nucleotide bindingi136 – 139GTPUniRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:RT0653
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000913821 – 394Elongation factor TuAdd BLAST394

Proteomic databases

PRIDEiQ8KT95.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0653.

Structurei

3D structure databases

ProteinModelPortaliQ8KT95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 204tr-type GAdd BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 26G1By similarity8
Regioni60 – 64G2By similarity5
Regioni81 – 84G3By similarity4
Regioni136 – 139G4By similarity4
Regioni174 – 176G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGV. Bacteria.
COG0050. LUCA.
HOGENOMiHOG000229290.
KOiK02358.
OMAiIEMFHKE.
OrthoDBiPOG091H00LA.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8KT95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAKFERTK PHVNIGTIGH VDHGKTSLTA AITIILAKTG GAKATAYDQI
60 70 80 90 100
DAAPEEKERG ITISTAHVEY ETNNRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVVSAAD GPMPQTREHI LLAKQVGVPA MVVFLNKVDM VDDPDLLELV
160 170 180 190 200
EMEVRELLSK YGFPGNEIPI IKGSALQALE GKPEGEKAIN ELMNAVDSYI
210 220 230 240 250
PQPIRATDKP FLMPIEDVFS ISGRGTVVTG RVESGIIKVG EEIEIVGLKN
260 270 280 290 300
TQKTTCTGVE MFRKLLDEGQ SGDNVGILLR GTKREEVERG QVLAKPGSIK
310 320 330 340 350
PHDKFEAEVY VLSKEEGGRH TPFTNDYRPQ FYFRTTDVTG TIKLPFDKQM
360 370 380 390
VMPGDNATFS VELIKPIAMQ EGLKFSIREG GRTVGAGVVT RINN
Length:394
Mass (Da):42,981
Last modified:November 9, 2004 - v2
Checksum:i457A55682ACA9CCC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225G → S in AAM90944 (PubMed:12140235).Curated1
Sequence conflicti269G → R in AAM90944 (PubMed:12140235).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF502186 Genomic DNA. Translation: AAM90944.1.
AE017197 Genomic DNA. Translation: AAU04116.1.
RefSeqiWP_011191093.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04116; AAU04116; RT0653.
KEGGirty:RT0653.
PATRICi17910548. VBIRicTyp34752_0673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF502186 Genomic DNA. Translation: AAM90944.1.
AE017197 Genomic DNA. Translation: AAU04116.1.
RefSeqiWP_011191093.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ8KT95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0653.

Proteomic databases

PRIDEiQ8KT95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU04116; AAU04116; RT0653.
KEGGirty:RT0653.
PATRICi17910548. VBIRicTyp34752_0673.

Phylogenomic databases

eggNOGiENOG4105CGV. Bacteria.
COG0050. LUCA.
HOGENOMiHOG000229290.
KOiK02358.
OMAiIEMFHKE.
OrthoDBiPOG091H00LA.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFTU_RICTY
AccessioniPrimary (citable) accession number: Q8KT95
Secondary accession number(s): Q68W76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: November 9, 2004
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.