Q8KR84 (Q8KR84_9ENTR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Isomaltulose synthase EMBL AAK82938.1
Organism	Klebsiella sp. LX3 EMBL AAK82938.1
Taxonomic identifier	167956 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 1M53
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	241	1	Important for catalytic activity PDB 1M53
	Site	295	1	Important for catalytic activity PDB 1M53

Sequences

	Sequence		Length	Mass (Da)
	Q8KR84 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 9F1611F9D0DFE28E	FASTA	598	69,930
	10 20 30 40 50 60 MSFVTLRTGV AVALSSLIIS LACPAVSAAP SLNQDIHVQK ESEYPAWWKE AVFYQIYPRS 70 80 90 100 110 120 FKDTNDDGIG DIRGIIEKLD YLKSLGIDAI WINPHYDSPN TDNGYDISNY RQIMKEYGTM 130 140 150 160 170 180 EDFDSLVAEM KKRNMRLMID VVINHTSDQH PWFIQSKSDK NNPYRDYYFW RDGKDNQPPN 190 200 210 220 230 240 NYPSFFGGSA WQKDAKSGQY YLHYFARQQP DLNWDNPKVR EDLYAMLRFW LDKGVSGMRF 250 260 270 280 290 300 DTVATYSKIP GFPNLTPEQQ KNFAEQYTMG PNIHRYIQEM NRKVLSRYDV ATAGEIFGVP 310 320 330 340 350 360 LDRSSQFFDR RRHELNMAFM FDLIRLDRDS NERWRHKSWS LSQFRQIISK MDVTVGKYGW 370 380 390 400 410 420 NTFFLDNHDN PRAVSHFGDD RPQWREASAK ALATITLTQR ATPFIYQGSE LGMTNYPFRQ 430 440 450 460 470 480 LNEFDDIEVK GFWQDYVQSG KVTATEFLDN VRLTSRDNSR TPFQWNDTLN AGFTRGKPWF 490 500 510 520 530 540 HINPNYVEIN AEREETREDS VLNYYKKMIQ LRHHIPALVY GAYQDLNPQD NTVYAYTRTL 550 560 570 580 590 GNERYLVVVN FKEYPVRYTL PANDAIEEVV IDTQQQAAAP HSTSLSLSPW QAGVYKLR

References

[1]	"Isomaltulose synthase from Klebsiella sp. strain LX3: gene cloning and characterization and engineering of thermostability." Zhang D., Li X., Zhang L.H. Appl. Environ. Microbiol. 68:2676-2682(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: LX3 EMBL AAK82938.1.
[2]	"Isomaltulose synthase (PalI) of Klebsiella sp. LX3. Crystal structure and implication of mechanism." Zhang D., Li N., Lok S.M., Zhang L.H., Swaminathan K. J. Biol. Chem. 278:35428-35434(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 29-598, ACTIVE SITE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY040843 Genomic DNA. Translation: AAK82938.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M53	X-ray	2.20	A	29-598	[»]

ProteinModelPortal

Q8KR84.

SMR

Q8KR84. Positions 43-598.

ModBase

Search...

Protein family/group databases

CAZy

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.

InterPro

IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

PANTHER

PTHR10357. PTHR10357. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
[Graphical view]

SMART

SM00642. Aamy. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8KR84.

Entry information

Entry name

Q8KR84_9ENTR

Accession

Primary (citable) accession number: Q8KR84

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)