Reviewed, UniProtKB/Swiss-Prot Q8KN28 (TFDB_DELAC)

Names and origin

Protein names

Recommended name:
    2,4-dichlorophenol 6-monooxygenase
    EC=1.14.13.20
Alternative name(s):
    2,4-dichlorophenol hydroxylase
      Short name=2,4-DCP hydroxylase

Gene names

Name:

tfdB

Organism

Delftia acidovorans (Pseudomonas acidovorans) (Comamonas acidovorans)

Taxonomic identifier

80866 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Delftia

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transforms 2,4-dichlorophenol (2,4-DCP) into 3,5-dichlorocatechol By similarity. UniProtKB P27138
Catalytic activity	2,4-dichlorophenol + NADPH + O2 = 3,5-dichlorocatechol + NADP+ + H2O. UniProtKB P27138
Cofactor	FAD By similarity. UniProtKB P27138
Pathway	Xenobiotic degradation; 2,4-dichlorophenoxyacetic acid degradation.
Subunit structure	Homotetramer By similarity. UniProtKB P27138
Sequence similarities	Belongs to the pheA/tfdB FAD monooxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,4-dichlorophenol 6-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	2,4-dichlorophenol 6-monooxygenase		PRO_0000214050
Regions
	Nucleotide binding	11 – 40	30	FAD Potential
	Nucleotide binding	304 – 314	11	FAD Potential
Experimental info
	Sequence conflict	2	1	Missing AA sequence Ref.4
	Sequence conflict	18	1	G → A AA sequence Ref.4
	Sequence conflict	195	1	M → V in AAD55079. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8KN28-1 [UniParc]. Last modified May 24, 2004. Version 3. Checksum: 6BEE58517E7B860E	FASTA	586	64,405
	10 20 30 40 50 60 MNEKATVIET DVLVVGSGPA GAASTLLLAT YGVKTLCVSK YATTSRTPRS HITNQRTMEV 70 80 90 100 110 120 MRDLGLELEC EAMASPAELM GENVYCTSLV GDELGRVLTW GTHPQRRADY ELASPTHMCD 130 140 150 160 170 180 LPQNLLEPIM INHAARRGAD VRFHTEFVSL KQDETGVTAT VRDHLLDRQY DIRAKYLIGA 190 200 210 220 230 240 DGANSQVVDQ VGLPMEGKMG VSGSINVVFE ADLTKYVGHR PSVLYWVIQP GSSVGGLGIG 250 260 270 280 290 300 VIRMVRPWNK WLCIWGYDIA GGPPDLNEAH ARQIVHSLLG DSTIPVKIES TSTWTVNDMY 310 320 330 340 350 360 ATRLFDNRVF CMGDAVHRHP PTNGLGSNTS IQDAFNLCWK LSHVLQGKAG PELLATYNEE 370 380 390 400 410 420 RAPVARQVVQ RANKSLGDFP PILAALGLFD TKDPEQMQRN IARLKEQSPE AQEQRAALRA 430 440 450 460 470 480 AIDGTQYVYN AHGVEMNQRY QSAAIVPDGT PDPGFRRDSE LYHAHSGRPG APVPHVWVTR 490 500 510 520 530 540 HGRRVSTLDL CGKGRFSLLS GIAGSPWVEA AVHAAESLGI DLDVHIIGPG QELEDLYGDF 550 560 570 580 ARVREIEESG ALLVRPDNFI CWRAMRWQEG SGDELRAALK RVLSVH

References

[1]	"A transposon encoding the complete 2,4-dichlorophenoxyacetic acid degradation pathway in the alkalitolerant strain Delftia acidovorans P4a." Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W. Microbiology 149:2545-2556(2003) [PubMed: 12949179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: P4a.
[2]	"Development and application of PCR primers for the detection of the tfd genes in Delftia acidovorans P4a involved in the degradation of 2,4-D." Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W. Acta Biotechnol. 21:321-331(2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-434. Strain: P4a.
[3]	"Physiological and genetic characteristics of two bacterial strains utilizing phenoxypropionate and phenoxyacetate herbicides." Mueller R.H., Kleinsteuber S., Babel W. Microbiol. Res. 156:121-131(2001) [PubMed: 11572451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-432. Strain: MC1.
[4]	"Regulation of catabolic enzymes during long-term exposure of Delftia acidovorans MC1 to chlorophenoxy herbicides." Benndorf D., Davidson I., Babel W. Microbiology 150:1005-1014(2004) [PubMed: 15073309] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-19. Strain: MC1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY078159 Genomic DNA. Translation: AAM76774.1. AF176242 Genomic DNA. Translation: AAD55079.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.20. 140899.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

TFDB_DELAC

Accession

Primary (citable) accession number: Q8KN28
Secondary accession number(s): Q93T15, Q9RP01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 24, 2004
Last modified:	March 24, 2009
This is version 37 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)