Q8KMJ5 (MCH_METED) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methenyltetrahydromethanopterin cyclohydrolase
EC=3.5.4.27

Alternative name(s):
Methenyl-H4MPT cyclohydrolase

Gene names

Name:	mch
Synonyms:	mchA
Ordered Locus Names:	METDI2515

Organism

Methylobacterium extorquens (strain DSM 5838 / DM4) (Methylobacterium dichloromethanicum (strain DM4)) [Complete proteome] [HAMAP]

Taxonomic identifier

661410 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of methenyl-H4MPT+ to 5-formyl-H4MPT By similarity. HAMAP MF_00486
Catalytic activity	5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin. HAMAP MF_00486
Pathway	One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (H(4)MPT route): step 3/5. HAMAP MF_00486
Subcellular location	Cytoplasm By similarity HAMAP MF_00486.
Sequence similarities	Belongs to the MCH family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methenyltetrahydromethanopterin cyclohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Methenyltetrahydromethanopterin cyclohydrolase HAMAP MF_00486		PRO_0000140894

Sequences

	Sequence		Length	Mass (Da)
	Q8KMJ5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 900FF2379E84F531	FASTA	323	33,268
	10 20 30 40 50 60 MSSNTSAPSL NALAGPLVES LVADAAKLRL IVAQENGART VDAGANARGS IEAGRRIAEI 70 80 90 100 110 120 CLGGLGTVTI APIGPVASWP YTVVVHSADP VLACLGSQYA GWSLADEDGD SGFFALGSGP 130 140 150 160 170 180 GRAVAVVEEL YKELGYRDNA TTTALVLESG SAPPASVVNK VAAATGLAPE NVTFIYAPTQ 190 200 210 220 230 240 SLAGSTQVVA RVLEVALHKA HTVGFDLHKI LDGIGSAPLS PPHPDFIQAM GRTNDAIIYG 250 260 270 280 290 300 GRVQLFVDAD DADAKQLAEQ IPSTTSADHG APFAEIFSRV NGDFYKIDGA LFSPAEAIVT 310 320 SVKTGKSFRG GRLEPQLVDA SFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dichloromethane metabolism and C1 utilization genes in Methylobacterium strains." Kayser M.F., Ucurum Z., Vuilleumier S. Microbiology 148:1915-1922(2002) [PubMed: 12055310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources." Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E. , Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C., Lidstrom M.E. PLoS ONE 4:E5584-E5584(2009) [PubMed: 19440302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5838 / DM4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ421477 Genomic DNA. Translation: CAD13315.1. FP103042 Genomic DNA. Translation: CAX24170.1.
RefSeq	YP_003068052.1. NC_012988.1.
3D structure databases
ProteinModelPortal	Q8KMJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8KMJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8192473.
GenomeReviews	Gene locus METDI2515 in contig FP103042_GR.
KEGG	mdi:METDI2515.
PATRIC	22523625. VBIMetExt143287_2345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YFAMGSG.
ProtClustDB	PRK02264.
Family and domain databases
HAMAP	MF_00486. McH. [Tree]
InterPro	IPR003209. METHMP_CycHdrlase. [Graphical view]
KO	K01499.
Pfam	PF02289. MCH. 1 hit. [Graphical view]
SUPFAM	SSF56199. Cyclohydrolase. 1 hit.
TIGRFAMs	TIGR03120. One_C_mch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCH_METED

Accession

Primary (citable) accession number: Q8KMJ5
Secondary accession number(s): C7CCN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program