Reviewed, UniProtKB/Swiss-Prot Q8KMJ5 (MCH_METDI)

Names and origin

Protein names

Recommended name:
    Methenyltetrahydromethanopterin cyclohydrolase
    EC=3.5.4.27
Alternative name(s):
    Methenyl-H4MPT cyclohydrolase

Gene names

Name:	mch
Synonyms:	mchA

Organism

Methylobacterium dichloromethanicum

Taxonomic identifier

119024 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of methenyl-H4MPT+ to 5-formyl-H4MPT By similarity.
Catalytic activity	5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin. HAMAP MF_00486
Pathway	One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (H(4)MPT route): step 3/5. HAMAP MF_00486
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MCH family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methenyltetrahydromethanopterin cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Methenyltetrahydromethanopterin cyclohydrolase HAMAP MF_00486		PRO_0000140894

Sequences

	Sequence		Length	Mass (Da)
	Q8KMJ5-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 900FF2379E84F531	FASTA	323	33,268
	10 20 30 40 50 60 MSSNTSAPSL NALAGPLVES LVADAAKLRL IVAQENGART VDAGANARGS IEAGRRIAEI 70 80 90 100 110 120 CLGGLGTVTI APIGPVASWP YTVVVHSADP VLACLGSQYA GWSLADEDGD SGFFALGSGP 130 140 150 160 170 180 GRAVAVVEEL YKELGYRDNA TTTALVLESG SAPPASVVNK VAAATGLAPE NVTFIYAPTQ 190 200 210 220 230 240 SLAGSTQVVA RVLEVALHKA HTVGFDLHKI LDGIGSAPLS PPHPDFIQAM GRTNDAIIYG 250 260 270 280 290 300 GRVQLFVDAD DADAKQLAEQ IPSTTSADHG APFAEIFSRV NGDFYKIDGA LFSPAEAIVT 310 320 SVKTGKSFRG GRLEPQLVDA SFV

References

[1]	"Dichloromethane metabolism and C1 utilization genes in Methylobacterium strains." Kayser M.F., Ucurum Z., Vuilleumier S. Microbiology 148:1915-1922(2002) [PubMed: 12055310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DM4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ421477 Genomic DNA. Translation: CAD13315.1.
3D structure databases
HSSP	HSSP built from PDB template 1QLM based on UniProtKB P94954.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.4.27. 288916.
Family and domain databases
HAMAP	MF_00486. [Tree]
InterPro	IPR003209. METHMP_CycHdrlase. [Graphical view]
Pfam	PF02289. MCH. 1 hit. [Graphical view]
ProDom	PD011637. Cyclohydrolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03120. one_C_mch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCH_METDI

Accession

Primary (citable) accession number: Q8KMJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)