Reviewed, UniProtKB/Swiss-Prot Q8KLU8 (ACEK_RALEH)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	H16_A0168

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057905
Regions
	Nucleotide binding	328 – 334	7	ATP By similarity
Sites
	Active site	384	1	By similarity
	Binding site	349	1	ATP By similarity
Experimental info
	Sequence conflict	275	1	E → K in CAC80133. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8KLU8-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2307038F15A1CD03	FASTA	613	71,480
	10 20 30 40 50 60 MSHFPKLLSS QIAFDVARTM LDGFDKHYRL FREVSHQAKL KFEAGDWHGL QQIQRDRIAF 70 80 90 100 110 120 YNERVRESSV ILEDEYDAEN IEDEIWQQIK LHYIGLLTNH HQPELAETFF NSVCTRILHR 130 140 150 160 170 180 SYFNNDFIFV RPAISTEYIE NEESPTRPTF RAYYPGSREG MAACFERVVH NFQLESPFED 190 200 210 220 230 240 LQRDIGYVVR AVSEHFGDLR IAPNFQVHTL SSLFFRNKSA FIIGRILNGD RTFPLAIPIV 250 260 270 280 290 300 HGPSGKLTLD TVLLKKEQLL ILFSFTHSYF MVDMEIPSAY VTFLRDIMPR KPRAEIYTSL 310 320 330 340 350 360 GLQKQGKNLF YRDFLHHLQH SSDKFIVAPG IRGLVMLVFT LPSYPYVFKV IKDFFPAPKE 370 380 390 400 410 420 TTRELVKSKY QLVKQHDRVG RMADTLEYSD VAFPLSRFDE ALVREFEQHA PSMIEYQRGK 430 440 450 460 470 480 DGGEEIVVRH VYIERRMTPL NIYLTEGTDE QVEHGVIEYG NAVKELIAAN IFPGDMLYKN 490 500 510 520 530 540 FGVTRHGRVV FYDYDEIEYL TDCNIRDVPQ PRNEEEEMSG EVWYTVRPHD IFPETFRTFL 550 560 570 580 590 600 LGDTRVRAAF LRHHADFFDP AMWQSHKDRL LAGHVHDFFA YHSSERFIHR YSEAGSAQGT 610 AAVPDPGPAR RVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Carbonic anhydrase is essential for growth of Ralstonia eutropha at ambient CO2 concentrations." Kusian B., Sultemeyer D., Bowien B. J. Bacteriol. 184:5018-5026(2002) [PubMed: 12193617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ310671 Genomic DNA. Translation: CAC80133.1. AM260479 Genomic DNA. Translation: CAJ91320.1.
RefSeq	YP_724688.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8KLU8.
Genome annotation databases
GeneID	4247137.
GenomeReviews	Gene locus H16_A0168 in contig AM260479_GR.
KEGG	reh:H16_A0168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KLU8.
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_RALEH

Accession

Primary (citable) accession number: Q8KLU8
Secondary accession number(s): Q0KFA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)