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Q8KLH5 (FIXP_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cbb3-type cytochrome c oxidase subunit FixP
Short name=Cbb3-Cox subunit FixP
Alternative name(s):
C-type cytochrome FixP
Short name=Cyt c(FixP)
Cytochrome c oxidase subunit III
Gene names
Name:fixP
Ordered Locus Names:RHE_PD00293
Encoded onPlasmid sym p42d
OrganismRhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]
Taxonomic identifier347834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium ›

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

C-type cytochrome. Part of the cbb3-type cytochrome c oxidase complex. FixP subunit is required for transferring electrons from donor cytochrome c via its heme groups to FixO subunit. From there, electrons are shuttled to the catalytic binuclear center of FixN subunit where oxygen reduction takes place. The complex also functions as a proton pump. Ref.1

Cofactor

Binds 2 heme C groups per subunit By similarity.

Pathway

Energy metabolism; oxidative phosphorylation.

Subunit structure

Component of the cbb3-type cytochrome c oxidase at least composed of FixN, FixO, FixQ and FixP By similarity.

Subcellular location

Cell inner membrane; Single-pass membrane protein By similarity.

Sequence similarities

Belongs to the CcoP / FixP family.

Contains 2 cytochrome c domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Cbb3-type cytochrome c oxidase subunit FixP
PRO_0000412298

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421Helical; Potential
Topological domain55 – 287233Periplasmic Potential
Domain108 – 19689Cytochrome c 1
Domain203 – 28482Cytochrome c 2

Sites

Metal binding1251Iron (heme C 1 axial ligand) By similarity
Metal binding1731Iron (heme C 2 axial ligand) By similarity
Metal binding2201Iron (heme C 2 axial ligand) By similarity
Metal binding2611Iron (heme C 1 axial ligand) By similarity
Binding site1211Heme C 1 (covalent) By similarity
Binding site1241Heme C 1 (covalent) By similarity
Binding site2161Heme C 2 (covalent) By similarity
Binding site2191Heme C 2 (covalent) By similarity

Natural variations

Natural variant11 – 122GV → VA in strain: CE3.
Natural variant431W → C in strain: CE3.
Natural variant45 – 462IG → DP in strain: CE3.
Natural variant247 – 2482DA → EP in strain: CE3.
Natural variant2781I → N in strain: CE3.
Natural variant282 – 2832AL → RF in strain: CE3.

Sequences

Sequence LengthMass (Da)Tools
Q8KLH5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 811F3C5E60022AE9

FASTA28730,767
        10         20         30         40         50         60 
MSQKHIDELS GVETTGHEWD GIQELNNPMP RWWIWTFYVT ILWAIGYAIA YPAIPMITSA 

        70         80         90        100        110        120 
TNGYLGYSTR AELQQDLNLA KSSQTEFHDL IAAKTVEEID ADPALRKFAI AGGASAFKVN 

       130        140        150        160        170        180 
CAPCHGSGAS GGPGFPNLND DDWLWGGDLN AIQATISHGI RFDGDTDSHS SEMPPFAGVL 

       190        200        210        220        230        240 
EPIQMKQVAA FVWGLTNTPS DVGLAAAGKQ VFFDNCAPCH GEDAKGKVEM GAPDLADAIW 

       250        260        270        280 
LKSRGEDAIL RQVASPKHGV MPAWAARLGD TTVNELTIFV HALGGGT

« Hide

References

« Hide 'large scale' references
[1]"Enhanced nitrogen fixation in a rhizobium etli ntrC mutant that overproduces the bradyrhizobium japonicum symbiotic terminal oxidase cbb3."
Soberon M., Lopez O., Morera C., Girard M.L., Tabche M.L., Miranda J.
Appl. Environ. Microbiol. 65:2015-2019(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: CE3.
[2]"The mosaic structure of the symbiotic plasmid of Rhizobium etli CFN42 and its relation to other symbiotic genome compartments."
Gonzalez V., Bustos P., Ramirez-Romero M.A., Medrano-Soto A., Salgado H., Hernandez-Gonzalez I., Hernandez-Celis J.C., Quintero V., Moreno-Hagelsieb G., Girard L., Rodriguez O., Flores M., Cevallos M.A., Collado-Vides J., Romero D., Davila G.
Genome Biol. 4:R36.1-R36.13(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.
[3]"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons."
Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G.
Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U76906 Genomic DNA. Translation: AAC15890.1.
U80928 Genomic DNA. Translation: AAM54763.1.
RefSeqNP_659749.1. NC_004041.2.

3D structure databases

HSSPHSSP built from PDB template 1KIB based on UniProtKB P00118.
ProteinModelPortalQ8KLH5.
ModBaseSearch...

Protein-protein interaction databases

STRING347834.RHE_PD00293.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM54763; AAM54763; RHE_PD00293.
GeneID1005009.
KEGGret:RHE_PD00293.
PATRIC23081154. VBIRhiEtl108884_0270.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2010.
HOGENOMHOG000277941.
KOK00406.
OMAFADNCAP.
ProtClustDBCLSK864483.

Enzyme and pathway databases

BioCycRETL347834:GJJ0-5509-MONOMER.
UniPathwayUPA00705.

Family and domain databases

InterProIPR009056. Cyt_c_dom.
IPR008168. Cyt_C_IC.
IPR004678. Cyt_c_oxidase_cbb3_su3.
[Graphical view]
PIRSFPIRSF000006. Cbb3-Cox_fixP. 1 hit.
PRINTSPR00605. CYTCHROMECIC.
SUPFAMSSF46626. Cytochrome_c. 2 hits.
TIGRFAMsTIGR00782. ccoP. 1 hit.
PROSITEPS51007. CYTC. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFIXP_RHIEC
AccessionPrimary (citable) accession number: Q8KLH5
Secondary accession number(s): O69780
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: October 1, 2002
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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