Q8KKG0 (Q8KKG0_9FLAO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Cyclomaltodextrinase EMBL CAD32957.1
EC=3.2.1.54 EMBL CAD32957.1

Gene names

Name:

cdase EMBL CAD32957.1

Organism

Flavobacterium sp. 92 EMBL CAD32957.1

Taxonomic identifier

197856 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL CAD32957.1
Ligand	Calcium PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK Metal-binding PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
Molecular function	Glycosidase EMBL CAD32957.1 Hydrolase
Technical term	3D-structure PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cyclomaltodextrinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	EMBL CAD32957.1
	Chain	19 – 619	601	cyclomaltodextrinase EMBL CAD32957.1		PRO_5000068586
Sites
	Metal binding	137	1	Calcium 1 PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	139	1	Calcium 1; via carbonyl oxygen PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	142	1	Calcium 1 PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	143	1	Calcium 1 PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	162	1	Calcium 1; via carbonyl oxygen PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	164	1	Calcium 1 PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	240	1	Calcium 2 PDB 1H3G PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	288	1	Calcium 2 PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	298	1	Calcium 2 PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK
	Metal binding	333	1	Calcium 2; via carbonyl oxygen PDB 1H3G PDB 3EDD PDB 3EDE PDB 3EDF PDB 3EDJ PDB 3EDK

Sequences

	Sequence		Length	Mass (Da)
	Q8KKG0 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3EC7A42471AE7FE7	FASTA	619	70,029
	10 20 30 40 50 60 MRRSFATLFL PTWPAPLPAA PTAIEHMEPP FWWAGMQHKG LQLMVHGRDI GRMEAALDYP 70 80 90 100 110 120 GVRLVSTTRV PNANYLFVDL EIGPEAQPGS FDIVFKGDGR SERYRYRLLA REQGSAQRQG 130 140 150 160 170 180 FGPGDAIYQI MPDRFANGDP SNDNVAGMRE QADRRHGGGR HGGDIRGTID HLDYIAGLGF 190 200 210 220 230 240 TQLWPTPLVE NDAAAYSYHG YAATDHYRID PRYGSNEDFV RLSTEARKRG MGLIQDVVLS 250 260 270 280 290 300 HIGKHHWWMK DLPTPDWINY GGKFVPTQHH RVAVQDPYAA QADSENFTKG WFVEGMPDLN 310 320 330 340 350 360 QTNPLVANYL IQNNIWWIEY AGLSGLRIDT YGYSDGAFLT EYTRRLMAEY PRLNMVGEEW 370 380 390 400 410 420 STRVPVVARW QRGKANFDGY TSHLPSLMDF PLVDAMRNAL SKTGEENGLN EVYETLSLDY 430 440 450 460 470 480 LYPEPQNLVL FGGNHDMARM FSAAGEDFDR WRMNLVFLMT MPRIPQFYSG DEILMTSTVK 490 500 510 520 530 540 GRDDASYRRD FPGGWAGDKA NAFSGAGLTS QQRAAQDLVR KLANWRKNQP VIHNGRLMHF 550 560 570 580 590 600 GPEENTWVYF RYNKDKRIMV AMNNNDKPMT LPTARFQEML KGAPSGVDFL SGKTVGLGRE 610 LRLAPKSVVV IELPGLPEA

References

[1]	"Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92." Fritzsche H.B., Schwede T., Schulz G.E. Eur. J. Biochem. 270:2332-2341(2003) [PubMed: 12752453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 92 EMBL CAD32957.1.
[2]	"Structural base for enzymatic cyclodextrin hydrolysis." Buedenbender S., Schulz G.E. J. Mol. Biol. 385:606-617(2009) [PubMed: 19014948] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 19-619 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ489171 Genomic DNA. Translation: CAD32957.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H3G	X-ray	2.10	A/B	20-619	[»]
3EDD	X-ray	2.65	A/B	19-619	[»]
3EDE	X-ray	1.71	A/B	19-619	[»]
3EDF	X-ray	1.65	A/B	19-619	[»]
3EDJ	X-ray	1.69	A/B	19-619	[»]
3EDK	X-ray	1.77	A/B	19-619	[»]

ProteinModelPortal

Q8KKG0.

SMR

Q8KKG0. Positions 21-617.

ModBase

Search...

Protein family/group databases

CAZy

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.2.1.54. 3795.

Family and domain databases

InterPro

IPR015902. Alpha_amylase.
IPR015171. Cyc-maltodext_N.
IPR019492. Cyclo-malto-dextrinase_C.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF10438. Cyc-maltodext_C. 1 hit.
PF09087. Cyc-maltodext_N. 1 hit.
[Graphical view]

SMART

SM00642. Aamy. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.
SSF81296. Ig_E-set. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q8KKG0_9FLAO

Accession

Primary (citable) accession number: Q8KKG0

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)