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Q8KGB3 (Q8KGB3_CHLTE) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL AAM71303.1
Ordered Locus Names:CT0055
OrganismChlorobium tepidum
Taxonomic identifier1097 [NCBI]
Taxonomic lineageBacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length799 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q8KGB3 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 2B5C452D35ED7F0C

FASTA79989,971
        10         20         30         40         50         60 
MASKSATPSA RNRTLIVVES PSKAKTINKY LGDDYTVYAS VGHIKDLPKK EIGIDFDNHY 

        70         80         90        100        110        120 
EPRYEVIAGK EKVVRQLKKL AGEADKVLIA TDPDREGEAI AWHISNEIDF AKKPVFRVLF 

       130        140        150        160        170        180 
NEITRNAIIE AIGHPQQIDY RLVRSQQTRQ ALDKIVGYKI SPFLWNVVYR GLSAGRVQSV 

       190        200        210        220        230        240 
VLRLICERET EIEAFTPQEY WTIYADFTTE NGETFRTKLV KVNGEKPDIT SGEQAEAIAS 

       250        260        270        280        290        300 
AIQGRLFAIS EIVPKVIQRK QPLPFTTSLL QQAASNQLGF GSQKTMRTAQ QLYEGIDLGS 

       310        320        330        340        350        360 
EGATGLITYM RTDSTRISGE AVAQAHDYIS QQFGPEYKGF GGQGKAGKNA QDAHEAIRPT 

       370        380        390        400        410        420 
SVTRTPESLR RHLTPDQFRL YELIWKRFVA SMMAPAKIEQ TRVDVDDQKK EFVFRATGNK 

       430        440        450        460        470        480 
VLFPGFFRVY DDQQELDYEA QKSTREELEK EQIVKLPERL SVDEQLKLAE LDRRQSFTRP 

       490        500        510        520        530        540 
PARYTEASLV KELDNYGIGR PSTYASIFST LQDRRYVELQ KKKIIPTELG RDVSAILVAN 

       550        560        570        580        590        600 
FPDLFNVRFT AEMEDELDKV ASGDDEYEKV LDRFYRPLES VLSHRKSDPL IPQNRDAVLC 

       610        620        630        640        650        660 
EKCGKGHMIV KWTTSGKFLG CSNYPKCKNI KAITTNKAKP KETGVRCPVC GEGHMLLRNG 

       670        680        690        700        710        720 
RLGPFLACSN YPKCNTLLNL SKQRHIEPIK TPPITTDLAC PKCGAPMYLR TGKRGLWLGC 

       730        740        750        760        770        780 
SKFPKCRGRL SWSTLDPETQ ARWEKAMNEH MAAHPSLAIT MLDGKPAPMN LPIEDIIARA 

       790 
EDAGLISATS EQPEAEATT

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006470 Genomic DNA. Translation: AAM71303.1.
RefSeqNP_660961.1. NC_002932.3.

3D structure databases

HSSPHSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortalQ8KGB3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1006473.
GenomeReviewsGene locus CT0055 in contig AE006470_GR.
KEGGcte:CT0055.
NMPDRfig|194439.1.peg.55.
PATRIC21398213. VBIChlTep116050_0055.
TIGRCT0055.

Phylogenomic databases

HOGENOMHBG512935.
OMAEEYWSID.
ProtClustDBPRK06599.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ8KGB3_CHLTE
AccessionPrimary (citable) accession number: Q8KGB3
Entry history
Integrated into UniProtKB/TrEMBL: October 1, 2002
Last sequence update: October 1, 2002
Last modified: December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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