Reviewed, UniProtKB/Swiss-Prot Q8KGA2 (CARA_CHLTE)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	CT0066

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112266
Regions
	Domain	178 – 366	189	Glutamine amidotransferase type-1
	Region	1 – 174	174	CPSase HAMAP MF_01209
Sites
	Active site	256	1	Nucleophile By similarity
	Active site	340	1	By similarity
	Active site	342	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KGA2-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 64AE575C1B19E3FF	FASTA	366	40,111
	10 20 30 40 50 60 MQEIPAILVL ENGSVYRGTA FGHAGEATGE VVFNTSLTGY QEILTDPSYT GQMVVMTYPL 70 80 90 100 110 120 IGNYGITPND NESKKIWASA LIVREASNVY SNYESTRSLD ATLKEAGVMG LAGIDTRKLV 130 140 150 160 170 180 REIRQKGAMR AVISTLCDDV AALKAQAAAI PEMTGLDLVQ RVTTGERYTV DNPDAKYHVV 190 200 210 220 230 240 AFDYGIKTNI IRQLNAEGCK VTVVNAKTTA DEVLAMNPDG IFLSNGPGDP FAVTYAIDTI 250 260 270 280 290 300 RELAARNSTL PIFGICLGHQ LLSLAFGAKT YKLKFGHHGA NHPVKNLLSN TIEITSQNHG 310 320 330 340 350 360 FAVEMESLPG ELELTHKNLY DMTVEGIRHR ELPCFSVQYH PEAAPGPHDS HYLFKEFTEL MERLKN

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM71314.1.
RefSeq	NP_660972.1.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1006486.
GenomeReviews	Gene locus CT0066 in contig AE006470_GR.
KEGG	cte:CT0066.
NMPDR	fig|194439.1.peg.66.
TIGR	CT0066.
Phylogenomic databases
HOGENOM	Q8KGA2.
OMA	Q8KGA2. LFDGSNC.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0066-MON.
BRENDA	6.3.5.5. 189605.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CHLTE

Accession

Primary (citable) accession number: Q8KGA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)