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Protein

NAD kinase

Gene

nadK

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei145 – 1451NADUniRule annotation
Binding sitei163 – 1631NADUniRule annotation
Binding sitei165 – 1651NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672NADUniRule annotation
Nucleotide bindingi134 – 1352NADUniRule annotation
Nucleotide bindingi176 – 1816NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-86-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:CT0085
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283NAD kinasePRO_0000120610Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi194439.CT0085.

Structurei

3D structure databases

ProteinModelPortaliQ8KG83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiFPRENFL.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KG83-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFAIFVNTT REKALELARE LTAWLDARSI DYVFDPQSAK ALGCGKWEEK
60 70 80 90 100
ADLSQHCDAF VALGGDGTLL LASHYSRSKP VVGINVGDLG FLTEFSPDEM
110 120 130 140 150
WVAMDHLVSG NYSIHTRSQL EATLESGESL TSLNDVIFEK GSAARRLPAF
160 170 180 190 200
TILLDDEMLG SYRADGIIIA TSTGSTAYSM SAGGPIIAPK SNVFVITPIC
210 220 230 240 250
PHMLTVRPIV ISDDKTIKIS VDSQSGEFPL KMDGIQKKLL APGEVVTVKK
260 270 280
SPHHINLVAN EKRNYCEILR KKLLWSHEHP TGE
Length:283
Mass (Da):30,989
Last modified:October 1, 2002 - v1
Checksum:iBD0615843D1A6A21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71333.1.
RefSeqiNP_660991.1. NC_002932.3.
WP_010931779.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71333; AAM71333; CT0085.
GeneIDi1006506.
KEGGicte:CT0085.
PATRICi21398273. VBIChlTep116050_0085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71333.1.
RefSeqiNP_660991.1. NC_002932.3.
WP_010931779.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KG83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT0085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM71333; AAM71333; CT0085.
GeneIDi1006506.
KEGGicte:CT0085.
PATRICi21398273. VBIChlTep116050_0085.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiFPRENFL.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-86-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / TLS.

Entry informationi

Entry nameiNADK_CHLTE
AccessioniPrimary (citable) accession number: Q8KG83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: October 1, 2002
Last modified: February 4, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.