Q8KG79 (CLPB1_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable chaperone protein ClpB 1

Gene names

Name:	clpB1
Ordered Locus Names:	CT0089

Organism

Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS) [Reference proteome] [HAMAP]

Taxonomic identifier

194439 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum › Chlorobaculum tepidum

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK By similarity.
Subunit structure	Homohexamer. The oligomerization is ATP-dependent By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer By similarity.
Sequence similarities	Belongs to the clpA/clpB family.
Caution	This protein is much smaller than the orthologs present in other bacteria; the N-terminal and NBD1 domains are missing. However, there is a paralog in the genome (clpB2) that encodes a protein which contains only the N-terminal and NBD1 domains.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Probable chaperone protein ClpB 1		PRO_0000191107
Regions
	Nucleotide binding	178 – 185	8	ATP Potential
	Region	1 – 118	118	Linker By similarity
	Region	128 – 345	218	NBD2 By similarity
	Region	346 – 438	93	C-terminal By similarity
	Coiled coil	1 – 94	94	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KG79 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C19CC552B2EA29FB	FASTA	438	49,655
	10 20 30 40 50 60 MNTADTRQRL LDIEKQIASL REEQATVKAQ WEAEKELIHT SRRLKEELED LRVQAENYER 70 80 90 100 110 120 SGDYGKVAEI RYGKIAEIEK ALEENNRKIE ARQASGDLIM KEEIDAGDIA DIVSRWTGIP 130 140 150 160 170 180 VSKMLQSERQ KLLGIESELH RRVVGQDEAV RAVSDAVKRS RAGMGDEKRP IGSFIFLGPT 190 200 210 220 230 240 GVGKTELART LAEYLFDDED ALIRIDMSEY MEAHTVSRLV GAPPGYVGYE EGGQLTEAVR 250 260 270 280 290 300 RKPFSVVLLD EIEKAHPDVF NILLQILDDG RLTDSKGRTV NFKNTIIIMT SNIGAQLIQS 310 320 330 340 350 360 EMEHLEGRDA DAALAGLKEK LFQLLKQQVR PEFLNRIDEV ILFTPLTREN LREIVTIQFN 370 380 390 400 410 420 RIKETARRQR ITLEISDEAL MWLAKTGFDP AFGARPLKRV MQRQITNRLS EMILAGQVGE 430 DDTVEIGLEN DAIVMKKK

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM71337.1.
RefSeq	NP_660995.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KG79.
ModBase	Search...
Protein-protein interaction databases
STRING	194439.CT0089.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM71337; AAM71337; CT0089.
GeneID	1006510.
KEGG	cte:CT0089.
PATRIC	21398281. VBIChlTep116050_0089.
Phylogenomic databases
eggNOG	COG0542.
HOGENOM	HOG000218211.
OMA	KRSRAGM.
ProtClustDB	CLSK637153.
Enzyme and pathway databases
BioCyc	CTEP194439:GHN0-90-MONOMER.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. [Graphical view]
Pfam	PF07724. AAA_2. 1 hit. PF10431. ClpB_D2-small. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 1 hit. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
PROSITE	PS00870. CLPAB_1. False negative. PS00871. CLPAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPB1_CHLTE

Accession

Primary (citable) accession number: Q8KG79

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program