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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
Active sitei609 – 6091For Fru-6P isomerization activityUniRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-132-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:CT0130
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 614613Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135320Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0130.

Structurei

3D structure databases

ProteinModelPortaliQ8KG38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 223222Glutamine amidotransferase type-2UniRule annotationAdd
BLAST
Domaini292 – 431140SIS 1UniRule annotationAdd
BLAST
Domaini463 – 604142SIS 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
Contains 2 SIS domains.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
KOiK00820.
OMAiSEFRYAP.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8KG38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIIGYIGR REAAPLLLNG LKRLEYRGYD SAGMAVLNGS MKMLKKKGSV
60 70 80 90 100
SNLEELLNVS GTVMLGATVG IAHTRWATHG DPSDRNAHPH MNVSGDIALI
110 120 130 140 150
HNGIIENYSA LKQELMGEGY VFESDTDSEV LVHLIDRIWK NDSALGLEGA
160 170 180 190 200
VRQALRHVEG AYGICVVSSR EPDKIVVARK GSPLVIGLGD GEFFIASDAA
210 220 230 240 250
PIVEHTNKVV YLSDGEMAVV TRDSYTVKTI ENVEQQKRVT ELDFSLEKIE
260 270 280 290 300
KGGFEHFMLK EIFEQPEVMR DVMRGRVRVE EGRVHLGGIH DYLDRLKQAK
310 320 330 340 350
RIMICACGTS WHAGLIGEYL IEEFARIPVE VDYASEFRYR NPIVSSDDVV
360 370 380 390 400
IVISQSGETA DTLAALRLAK EKGAMVMGIC NVVGSTIPRE TLCGMYTHAG
410 420 430 440 450
PEVGVASTKA FTAQVIVLFM LAMALSKGRT ISQEEIKLNL RELAEVPDKV
460 470 480 490 500
AWILEQNDAI KEIAVKLKDA RNALYLGRGY NFPVALEGAL KLKEISYIHA
510 520 530 540 550
EGYPAAEMKH GPIALIDEDM PVIVIATRDN TYAKILSNIE EVRSRKGRVI
560 570 580 590 600
AIASEGDREI ERLTEDVIYI PQASAAVLPL LTVIPLQLLS YHVATLRGCN
610
VDRPRNLAKS VTVE
Length:614
Mass (Da):67,577
Last modified:January 22, 2007 - v3
Checksum:iA9B4195C3DC3F84F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71378.1.
RefSeqiNP_661036.1. NC_002932.3.
WP_010931824.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71378; AAM71378; CT0130.
GeneIDi1006837.
KEGGicte:CT0130.
PATRICi21398359. VBIChlTep116050_0127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71378.1.
RefSeqiNP_661036.1. NC_002932.3.
WP_010931824.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KG38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT0130.

Protein family/group databases

MEROPSiC44.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM71378; AAM71378; CT0130.
GeneIDi1006837.
KEGGicte:CT0130.
PATRICi21398359. VBIChlTep116050_0127.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258898.
KOiK00820.
OMAiSEFRYAP.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-132-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / TLS.

Entry informationi

Entry nameiGLMS_CHLTE
AccessioniPrimary (citable) accession number: Q8KG38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2002
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.