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Q8KFL5

- SYI_CHLTE

UniProt

Q8KFL5 - SYI_CHLTE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CT0311
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10841084Isoleucine--tRNA ligasePRO_0000098532Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0311.

Structurei

3D structure databases

ProteinModelPortaliQ8KFL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6111"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KFL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPATYPEYPS SLSYSAMEAQ IREFWIERNI FRKSLEKDAP KGIYSFYEGP
60 70 80 90 100
PTVNGKPGVH HLFSRTIKDV VCRYHTMQGY QVPRKAGWDT HGLPVEISVE
110 120 130 140 150
KKLGLKNKSH VEEYGVGEFN REARALVYHH IDDNREGWGK LTERMGYWVD
160 170 180 190 200
MDSPYITCDN NYIESVWWAL KTIFDKGLIY KDYKIVPQDP KSETVLSSHE
210 220 230 240 250
LALGYKEVQD PSVYIKFRLK DSGESILVWT TTPWTLISNV ALAVGRDIDY
260 270 280 290 300
VRVKHRETGE VLILAESRLS VLVEKIGDES AWEVIDRCKG SDLEGRDYEP
310 320 330 340 350
LFNYFSPERR AWYVVCGDFV STGEGTGIVH IAPAFGADDY ELSKQYQLPM
360 370 380 390 400
LQPVARNGCF TAEVPDYEGM FFKDADKPIM QRLKEEGKLY RRETIQHTYP
410 420 430 440 450
FSWRYDVPVI YYARESWYIR TTDIAPRMVA LNKTINWNPP EIGTGRFGNW
460 470 480 490 500
LEENKDWALS RERFWGTPLP VWVAEDFAIG DGPDSGKLFA VGSVAELREG
510 520 530 540 550
FIEIDGEEMN LGDALDKGLV ELDLHKPFVD RIWFIRDGKR FNRTPELIDV
560 570 580 590 600
WFDSGSMPFA QLHYPFENKE LFDKTFPADF IAEGVDQTRG WFYTLHAIAT
610 620 630 640 650
LIFDRPAYRN VVVNGHILDK SGQKMSKSKG NVVDPFESME QYGADAIRWY
660 670 680 690 700
LMITSPPWRP KLFNAAEIEE EQRKFFRAFI NSYNFFVLYA NVDGFRYEEA
710 720 730 740 750
DIPFTQRSEL DRWVLSSLNT LIAEVTSRME QYDLTGACRL IGDFTVDDLS
760 770 780 790 800
NWYIRRSRKR FWKGEMGPDK LSAYQTLSTV LETLAKLMAP FVPFIAEKIW
810 820 830 840 850
LDLKSVGGTS KAESVHLADW PVADESCIDA ALEERMKKAQ IITSLVRTMR
860 870 880 890 900
EKAGIKVRQP LRRILLAAAE PGSRAAYELV SDIIKEEVNV QKIEYVEDED
910 920 930 940 950
GSVISKKAKP NFKTLGPRFG KDMKLLAEEI RIMSHKQISR LEKEGSIEID
960 970 980 990 1000
LGGRICTVLR EDVDIVHEDI EGWLVAADDA HRIMVALDTE ITEELEMLGL
1010 1020 1030 1040 1050
ARELVSRIQT LRKESGLEIT DRIALTIAGS EKLLAAARKS ESYIMDETLA
1060 1070 1080
TSIVLLPLDD SQPGDGVEQV NNELCRLSLE KSGS
Length:1,084
Mass (Da):124,097
Last modified:October 1, 2002 - v1
Checksum:i5CAB0E7BBD050655
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71557.1.
RefSeqiNP_661215.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71557; AAM71557; CT0311.
GeneIDi1007971.
KEGGicte:CT0311.
PATRICi21398737. VBIChlTep116050_0301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71557.1 .
RefSeqi NP_661215.1. NC_002932.3.

3D structure databases

ProteinModelPortali Q8KFL5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 194439.CT0311.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM71557 ; AAM71557 ; CT0311 .
GeneIDi 1007971.
KEGGi cte:CT0311.
PATRICi 21398737. VBIChlTep116050_0301.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTEP194439:GHN0-328-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / TLS.

Entry informationi

Entry nameiSYI_CHLTE
AccessioniPrimary (citable) accession number: Q8KFL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3