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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei627ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-328-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CT0311
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985321 – 1084Isoleucine--tRNA ligaseAdd BLAST1084

Proteomic databases

PRIDEiQ8KFL5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0311

Structurei

3D structure databases

ProteinModelPortaliQ8KFL5
SMRiQ8KFL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi51 – 61"HIGH" regionAdd BLAST11
Motifi624 – 628"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KFL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPATYPEYPS SLSYSAMEAQ IREFWIERNI FRKSLEKDAP KGIYSFYEGP
60 70 80 90 100
PTVNGKPGVH HLFSRTIKDV VCRYHTMQGY QVPRKAGWDT HGLPVEISVE
110 120 130 140 150
KKLGLKNKSH VEEYGVGEFN REARALVYHH IDDNREGWGK LTERMGYWVD
160 170 180 190 200
MDSPYITCDN NYIESVWWAL KTIFDKGLIY KDYKIVPQDP KSETVLSSHE
210 220 230 240 250
LALGYKEVQD PSVYIKFRLK DSGESILVWT TTPWTLISNV ALAVGRDIDY
260 270 280 290 300
VRVKHRETGE VLILAESRLS VLVEKIGDES AWEVIDRCKG SDLEGRDYEP
310 320 330 340 350
LFNYFSPERR AWYVVCGDFV STGEGTGIVH IAPAFGADDY ELSKQYQLPM
360 370 380 390 400
LQPVARNGCF TAEVPDYEGM FFKDADKPIM QRLKEEGKLY RRETIQHTYP
410 420 430 440 450
FSWRYDVPVI YYARESWYIR TTDIAPRMVA LNKTINWNPP EIGTGRFGNW
460 470 480 490 500
LEENKDWALS RERFWGTPLP VWVAEDFAIG DGPDSGKLFA VGSVAELREG
510 520 530 540 550
FIEIDGEEMN LGDALDKGLV ELDLHKPFVD RIWFIRDGKR FNRTPELIDV
560 570 580 590 600
WFDSGSMPFA QLHYPFENKE LFDKTFPADF IAEGVDQTRG WFYTLHAIAT
610 620 630 640 650
LIFDRPAYRN VVVNGHILDK SGQKMSKSKG NVVDPFESME QYGADAIRWY
660 670 680 690 700
LMITSPPWRP KLFNAAEIEE EQRKFFRAFI NSYNFFVLYA NVDGFRYEEA
710 720 730 740 750
DIPFTQRSEL DRWVLSSLNT LIAEVTSRME QYDLTGACRL IGDFTVDDLS
760 770 780 790 800
NWYIRRSRKR FWKGEMGPDK LSAYQTLSTV LETLAKLMAP FVPFIAEKIW
810 820 830 840 850
LDLKSVGGTS KAESVHLADW PVADESCIDA ALEERMKKAQ IITSLVRTMR
860 870 880 890 900
EKAGIKVRQP LRRILLAAAE PGSRAAYELV SDIIKEEVNV QKIEYVEDED
910 920 930 940 950
GSVISKKAKP NFKTLGPRFG KDMKLLAEEI RIMSHKQISR LEKEGSIEID
960 970 980 990 1000
LGGRICTVLR EDVDIVHEDI EGWLVAADDA HRIMVALDTE ITEELEMLGL
1010 1020 1030 1040 1050
ARELVSRIQT LRKESGLEIT DRIALTIAGS EKLLAAARKS ESYIMDETLA
1060 1070 1080
TSIVLLPLDD SQPGDGVEQV NNELCRLSLE KSGS
Length:1,084
Mass (Da):124,097
Last modified:October 1, 2002 - v1
Checksum:i5CAB0E7BBD050655
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM71557.1
RefSeqiNP_661215.1, NC_002932.3
WP_010932003.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM71557; AAM71557; CT0311
GeneIDi1007971
KEGGicte:CT0311
PATRICifig|194439.7.peg.301

Similar proteinsi

Entry informationi

Entry nameiSYI_CHLTE
AccessioniPrimary (citable) accession number: Q8KFL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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