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Q8KFL5

- SYI_CHLTE

UniProt

Q8KFL5 - SYI_CHLTE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CT0311
Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CT0311
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10841084Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098532Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0311.

Structurei

3D structure databases

ProteinModelPortaliQ8KFL5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6111"HIGH" regionUniRule annotation
Add
BLAST
Motifi624 – 6285"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KFL5-1 [UniParc]FASTAAdd to Basket

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MPATYPEYPS SLSYSAMEAQ IREFWIERNI FRKSLEKDAP KGIYSFYEGP     50
PTVNGKPGVH HLFSRTIKDV VCRYHTMQGY QVPRKAGWDT HGLPVEISVE 100
KKLGLKNKSH VEEYGVGEFN REARALVYHH IDDNREGWGK LTERMGYWVD 150
MDSPYITCDN NYIESVWWAL KTIFDKGLIY KDYKIVPQDP KSETVLSSHE 200
LALGYKEVQD PSVYIKFRLK DSGESILVWT TTPWTLISNV ALAVGRDIDY 250
VRVKHRETGE VLILAESRLS VLVEKIGDES AWEVIDRCKG SDLEGRDYEP 300
LFNYFSPERR AWYVVCGDFV STGEGTGIVH IAPAFGADDY ELSKQYQLPM 350
LQPVARNGCF TAEVPDYEGM FFKDADKPIM QRLKEEGKLY RRETIQHTYP 400
FSWRYDVPVI YYARESWYIR TTDIAPRMVA LNKTINWNPP EIGTGRFGNW 450
LEENKDWALS RERFWGTPLP VWVAEDFAIG DGPDSGKLFA VGSVAELREG 500
FIEIDGEEMN LGDALDKGLV ELDLHKPFVD RIWFIRDGKR FNRTPELIDV 550
WFDSGSMPFA QLHYPFENKE LFDKTFPADF IAEGVDQTRG WFYTLHAIAT 600
LIFDRPAYRN VVVNGHILDK SGQKMSKSKG NVVDPFESME QYGADAIRWY 650
LMITSPPWRP KLFNAAEIEE EQRKFFRAFI NSYNFFVLYA NVDGFRYEEA 700
DIPFTQRSEL DRWVLSSLNT LIAEVTSRME QYDLTGACRL IGDFTVDDLS 750
NWYIRRSRKR FWKGEMGPDK LSAYQTLSTV LETLAKLMAP FVPFIAEKIW 800
LDLKSVGGTS KAESVHLADW PVADESCIDA ALEERMKKAQ IITSLVRTMR 850
EKAGIKVRQP LRRILLAAAE PGSRAAYELV SDIIKEEVNV QKIEYVEDED 900
GSVISKKAKP NFKTLGPRFG KDMKLLAEEI RIMSHKQISR LEKEGSIEID 950
LGGRICTVLR EDVDIVHEDI EGWLVAADDA HRIMVALDTE ITEELEMLGL 1000
ARELVSRIQT LRKESGLEIT DRIALTIAGS EKLLAAARKS ESYIMDETLA 1050
TSIVLLPLDD SQPGDGVEQV NNELCRLSLE KSGS 1084
Length:1,084
Mass (Da):124,097
Last modified:October 1, 2002 - v1
Checksum:i5CAB0E7BBD050655
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006470 Genomic DNA. Translation: AAM71557.1.
RefSeqiNP_661215.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71557; AAM71557; CT0311.
GeneIDi1007971.
KEGGicte:CT0311.
PATRICi21398737. VBIChlTep116050_0301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006470 Genomic DNA. Translation: AAM71557.1 .
RefSeqi NP_661215.1. NC_002932.3.

3D structure databases

ProteinModelPortali Q8KFL5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 194439.CT0311.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM71557 ; AAM71557 ; CT0311 .
GeneIDi 1007971.
KEGGi cte:CT0311.
PATRICi 21398737. VBIChlTep116050_0301.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTEP194439:GHN0-328-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / TLS.

Entry informationi

Entry nameiSYI_CHLTE
AccessioniPrimary (citable) accession number: Q8KFL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi