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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-328-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CT0311
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10841084Isoleucine--tRNA ligasePRO_0000098532Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0311.

Structurei

3D structure databases

ProteinModelPortaliQ8KFL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6111"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiWGTPLNI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KFL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPATYPEYPS SLSYSAMEAQ IREFWIERNI FRKSLEKDAP KGIYSFYEGP
60 70 80 90 100
PTVNGKPGVH HLFSRTIKDV VCRYHTMQGY QVPRKAGWDT HGLPVEISVE
110 120 130 140 150
KKLGLKNKSH VEEYGVGEFN REARALVYHH IDDNREGWGK LTERMGYWVD
160 170 180 190 200
MDSPYITCDN NYIESVWWAL KTIFDKGLIY KDYKIVPQDP KSETVLSSHE
210 220 230 240 250
LALGYKEVQD PSVYIKFRLK DSGESILVWT TTPWTLISNV ALAVGRDIDY
260 270 280 290 300
VRVKHRETGE VLILAESRLS VLVEKIGDES AWEVIDRCKG SDLEGRDYEP
310 320 330 340 350
LFNYFSPERR AWYVVCGDFV STGEGTGIVH IAPAFGADDY ELSKQYQLPM
360 370 380 390 400
LQPVARNGCF TAEVPDYEGM FFKDADKPIM QRLKEEGKLY RRETIQHTYP
410 420 430 440 450
FSWRYDVPVI YYARESWYIR TTDIAPRMVA LNKTINWNPP EIGTGRFGNW
460 470 480 490 500
LEENKDWALS RERFWGTPLP VWVAEDFAIG DGPDSGKLFA VGSVAELREG
510 520 530 540 550
FIEIDGEEMN LGDALDKGLV ELDLHKPFVD RIWFIRDGKR FNRTPELIDV
560 570 580 590 600
WFDSGSMPFA QLHYPFENKE LFDKTFPADF IAEGVDQTRG WFYTLHAIAT
610 620 630 640 650
LIFDRPAYRN VVVNGHILDK SGQKMSKSKG NVVDPFESME QYGADAIRWY
660 670 680 690 700
LMITSPPWRP KLFNAAEIEE EQRKFFRAFI NSYNFFVLYA NVDGFRYEEA
710 720 730 740 750
DIPFTQRSEL DRWVLSSLNT LIAEVTSRME QYDLTGACRL IGDFTVDDLS
760 770 780 790 800
NWYIRRSRKR FWKGEMGPDK LSAYQTLSTV LETLAKLMAP FVPFIAEKIW
810 820 830 840 850
LDLKSVGGTS KAESVHLADW PVADESCIDA ALEERMKKAQ IITSLVRTMR
860 870 880 890 900
EKAGIKVRQP LRRILLAAAE PGSRAAYELV SDIIKEEVNV QKIEYVEDED
910 920 930 940 950
GSVISKKAKP NFKTLGPRFG KDMKLLAEEI RIMSHKQISR LEKEGSIEID
960 970 980 990 1000
LGGRICTVLR EDVDIVHEDI EGWLVAADDA HRIMVALDTE ITEELEMLGL
1010 1020 1030 1040 1050
ARELVSRIQT LRKESGLEIT DRIALTIAGS EKLLAAARKS ESYIMDETLA
1060 1070 1080
TSIVLLPLDD SQPGDGVEQV NNELCRLSLE KSGS
Length:1,084
Mass (Da):124,097
Last modified:October 1, 2002 - v1
Checksum:i5CAB0E7BBD050655
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71557.1.
RefSeqiNP_661215.1. NC_002932.3.
WP_010932003.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71557; AAM71557; CT0311.
GeneIDi1007971.
KEGGicte:CT0311.
PATRICi21398737. VBIChlTep116050_0301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71557.1.
RefSeqiNP_661215.1. NC_002932.3.
WP_010932003.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KFL5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT0311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM71557; AAM71557; CT0311.
GeneIDi1007971.
KEGGicte:CT0311.
PATRICi21398737. VBIChlTep116050_0301.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiWGTPLNI.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-328-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS.

Entry informationi

Entry nameiSYI_CHLTE
AccessioniPrimary (citable) accession number: Q8KFL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.