ID   PUR9_CHLTE              Reviewed;         523 AA.
AC   Q8KFK6;
DT   20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=Bifunctional purine biosynthesis protein purH;
DE   Includes:
DE     RecName: Full=Phosphoribosylaminoimidazolecarboxamide formyltransferase;
DE              EC=2.1.2.3;
DE     AltName: Full=AICAR transformylase;
DE   Includes:
DE     RecName: Full=IMP cyclohydrolase;
DE              EC=3.5.4.10;
DE     AltName: Full=Inosinicase;
DE     AltName: Full=IMP synthetase;
DE     AltName: Full=ATIC;
GN   Name=purH; OrderedLocusNames=CT0320;
OS   Chlorobium tepidum.
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC   Chlorobaculum.
OX   NCBI_TaxID=1097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49652 / DSM 12025 / TLS;
RX   MEDLINE=22103685; PubMed=12093901; DOI=10.1073/pnas.132181499;
RA   Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M.,
RA   Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F.,
RA   Holt I.E., Umayam L.A., Mason T.M., Brenner M., Shea T.P.,
RA   Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B.,
RA   Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M.,
RA   Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M.;
RT   "The complete genome sequence of Chlorobium tepidum TLS, a
RT   photosynthetic, anaerobic, green-sulfur bacterium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002).
CC   -!- CATALYTIC ACTIVITY: 10-formyltetrahydrofolate + 5-amino-1-(5-
CC       phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-
CC       formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
CC   -!- CATALYTIC ACTIVITY: IMP + H(2)O = 5-formamido-1-(5-phospho-D-
CC       ribosyl)imidazole-4-carboxamide.
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-
CC       amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl
CC       THF route): step 1/1.
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-
CC       carboxamide: step 1/1.
CC   -!- DOMAIN: The IMP cyclohydrolase activity resides in the N-terminal
CC       region (By similarity).
CC   -!- SIMILARITY: Belongs to the purH family.
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DR   EMBL; AE006470; AAM71566.1; -; Genomic_DNA.
DR   RefSeq; NP_661224.1; -.
DR   HSSP; P31335; 1G8M.
DR   GeneID; 1007981; -.
DR   GenomeReviews; AE006470_GR; CT0320.
DR   KEGG; cte:CT0320; -.
DR   NMPDR; fig|194439.1.peg.318; -.
DR   TIGR; CT0320; -.
DR   HOGENOM; Q8KFK6; -.
DR   OMA; Q8KFK6; VVKHVKS.
DR   BioCyc; CTEP194439:CT_0320-MON; -.
DR   BRENDA; 2.1.2.3; 189605.
DR   BRENDA; 3.5.4.10; 189605.
DR   GO; GO:0003937; F:IMP cyclohydrolase activity; IEA:HAMAP.
DR   GO; GO:0004643; F:phosphoribosylaminoimidazolecarboxamide for...; IEA:HAMAP.
DR   GO; GO:0006188; P:IMP biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_00139; -; 1.
DR   InterPro; IPR002695; AICARFT_IMPCHas.
DR   InterPro; IPR013982; AICARFT_IMPCHas_formly.
DR   InterPro; IPR011607; MGS.
DR   PANTHER; PTHR11692; AICARFT_IMPCHas; 1.
DR   Pfam; PF01808; AICARFT_IMPCHas; 1.
DR   Pfam; PF02142; MGS; 1.
DR   PIRSF; PIRSF000414; AICARFT_IMPCHas; 1.
DR   SMART; SM00798; AICARFT_IMPCHas; 1.
DR   TIGRFAMs; TIGR00355; purH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Multifunctional enzyme;
KW   Purine biosynthesis; Transferase.
FT   CHAIN         1    523       Bifunctional purine biosynthesis protein
FT                                purH.
FT                                /FTId=PRO_0000192082.
SQ   SEQUENCE   523 AA;  56743 MW;  EB17A1E65D679920 CRC64;
     MLDPVIKRAL VSVSDKTGIV DFCRELASMD VEIFSTGGTL KLLQGAGIVA QSISTITGFP
     EIMDGRVKTL HPKIHGGLLA VRDNAEHQKA ARENGIQFID LVVVNLYPFE ATIAKADVTF
     EEAIENIDIG GPSMLRSAAK NNESVTVVTD VADYATVLDE MRSNGGATTR ATRLTLAAKV
     YALTSRYDTA IAAYMAKAAG VKGAGDTMTL KLEKELSMRY GENPHQSAGL YKMDDGNGVR
     SFSAIFEKLH GKELSYNNML DIAAATGIIE EFRGEEPSVV IVKHTNPCGV AQAPTLCEAY
     RKAFSTDTQA PFGGIIAFNR PLDMETASAV NEIFTEILIA PAFEDGVLEM LMKKKDRRLV
     LQKQPLPKAG WEFKSTPFGM LVQERDSKTV APEELKVVTK RQPTAEELAD LMFAWKIVRH
     IKSNTILYVK NRQTFGVGAG QMSRVDSSKI ARWKASEVAL DLRGSVVASD AFFPFADGLL
     AAAEAGVTAV IQPGGSIRDN EVIEAADANN LAMVFTGMRH FKH
//