Reviewed, UniProtKB/Swiss-Prot Q8KFK6 (PUR9_CHLTE)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	CT0320

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity.
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_0000192082

Sequences

	Sequence		Length	Mass (Da)
	Q8KFK6-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: EB17A1E65D679920	FASTA	523	56,743
	10 20 30 40 50 60 MLDPVIKRAL VSVSDKTGIV DFCRELASMD VEIFSTGGTL KLLQGAGIVA QSISTITGFP 70 80 90 100 110 120 EIMDGRVKTL HPKIHGGLLA VRDNAEHQKA ARENGIQFID LVVVNLYPFE ATIAKADVTF 130 140 150 160 170 180 EEAIENIDIG GPSMLRSAAK NNESVTVVTD VADYATVLDE MRSNGGATTR ATRLTLAAKV 190 200 210 220 230 240 YALTSRYDTA IAAYMAKAAG VKGAGDTMTL KLEKELSMRY GENPHQSAGL YKMDDGNGVR 250 260 270 280 290 300 SFSAIFEKLH GKELSYNNML DIAAATGIIE EFRGEEPSVV IVKHTNPCGV AQAPTLCEAY 310 320 330 340 350 360 RKAFSTDTQA PFGGIIAFNR PLDMETASAV NEIFTEILIA PAFEDGVLEM LMKKKDRRLV 370 380 390 400 410 420 LQKQPLPKAG WEFKSTPFGM LVQERDSKTV APEELKVVTK RQPTAEELAD LMFAWKIVRH 430 440 450 460 470 480 IKSNTILYVK NRQTFGVGAG QMSRVDSSKI ARWKASEVAL DLRGSVVASD AFFPFADGLL 490 500 510 520 AAAEAGVTAV IQPGGSIRDN EVIEAADANN LAMVFTGMRH FKH

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM71566.1.
RefSeq	NP_661224.1.
3D structure databases
HSSP	HSSP built from PDB template 1G8M based on UniProtKB P31335.
ModBase	Search...
Genome annotation databases
GeneID	1007981.
GenomeReviews	Gene locus CT0320 in contig AE006470_GR.
KEGG	cte:CT0320.
NMPDR	fig|194439.1.peg.318.
TIGR	CT0320.
Phylogenomic databases
HOGENOM	Q8KFK6.
OMA	Q8KFK6. VVKHVKS.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0320-MON.
BRENDA	2.1.2.3. 189605. 3.5.4.10. 189605.
Family and domain databases
HAMAP	MF_00139. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHas_formly. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_CHLTE

Accession

Primary (citable) accession number: Q8KFK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)