Q8KFJ6 (NTPA_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

CT0330

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178151
Regions
	Region	14 – 19	6	Substrate binding By similarity
	Region	83 – 84	2	Substrate binding By similarity
Sites
	Metal binding	49	1	Magnesium or manganese By similarity
	Metal binding	83	1	Magnesium or manganese By similarity
	Binding site	179	1	Substrate By similarity
	Binding site	199	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KFJ6 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 2F5D995E8A69DC59	FASTA	226	24,411
	10 20 30 40 50 60 MEPKHPEITI VLATGNKDKV RELKPVLEAL ASGIHVRSLH DLGLDIDVEE TEPTLEGNAR 70 80 90 100 110 120 LKADAIFELV APRLDWFIAL ADDTGLEVDA LGGAPGVYSA RYAPVPEGVA RTYEDNVRHL 130 140 150 160 170 180 LSEMRGKSKR TARFRTVIAM KGRLPAANGS AVEIEETTDG HIDGLITTEP KGNGGFGYDP 190 200 210 220 VFAPEGMDRT FAQLGIDEKN AISHRGRAVV AAAKRIGEYL SQCGIQ

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM71576.1.
RefSeq	NP_661234.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KFJ6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1007992.
GenomeReviews	Gene locus CT0330 in contig AE006470_GR.
KEGG	cte:CT0330.
NMPDR	fig|194439.1.peg.328.
PATRIC	21398777. VBIChlTep116050_0320.
TIGR	CT0330.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	YSKRYDQ.
ProtClustDB	PRK14826.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0330-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_CHLTE

Accession

Primary (citable) accession number: Q8KFJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program