Q8KFG8 (F16PA_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-1,6-bisphosphatase class 1
Short name=FBPase class 1
EC=3.1.3.11

Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1

Gene names

Name:	fbp
Ordered Locus Names:	CT0358

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; Calvin cycle. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Calvin cycle Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Fructose-1,6-bisphosphatase class 1 HAMAP MF_01855		PRO_0000364525
Regions
	Region	116 – 119	4	Substrate binding By similarity
Sites
	Metal binding	92	1	Magnesium 1 By similarity
	Metal binding	113	1	Magnesium 1 By similarity
	Metal binding	113	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Metal binding	278	1	Magnesium 2 By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	272	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KFG8 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 98C24325F095CD09	FASTA	333	37,311
	10 20 30 40 50 60 MNKLTTIESH FLQLQKRYPE INSEVTDLLN DVAFAAKLVR REVVRAGLAD ILGLAGSTNV 70 80 90 100 110 120 QGEEVKKLDL FANERLINAI GQHGRFAIMG SEENEEIIKP PKFESGEYVL LFDPLDGSSN 130 140 150 160 170 180 IDVNVSVGTI FSIYRLKSGE PSQASLEDCL QKGADQIAAG YVIYGSSVMM VYTTGHGVHG 190 200 210 220 230 240 FTYDQTVGEF LLSHENITTP EHGKYYSVNE GSWQEFNDGT KRFLDYLKEE DKATGRPYST 250 260 270 280 290 300 RYIGSFVADF HRNLLTGGVF VYPATKKHKN GKLRLMYEAN PMAFICEQAG GRATDGYRRI 310 320 330 LDIEPKELHQ RTPLYIGSKN DVLIAEEFEQ GKR

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM71604.1.
RefSeq	NP_661262.1. NC_002932.3.
3D structure databases
HSSP	HSSP built from PDB template 2GQ1 based on UniProtKB P0A993.
ProteinModelPortal	Q8KFG8.
ModBase	Search...
Proteomic databases
PRIDE	Q8KFG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1008020.
GenomeReviews	Gene locus CT0358 in contig AE006470_GR.
KEGG	cte:CT0358.
NMPDR	fig|194439.1.peg.356.
PATRIC	21398827. VBIChlTep116050_0345.
TIGR	CT0358.
Phylogenomic databases
HOGENOM	HBG731261.
OMA	HWEAPVQ.
ProtClustDB	PRK09293.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0358-MONOMER.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. FBPase_class-1/SBPase. [Graphical view]
KO	K03841.
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PIRSF	PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16PA_CHLTE

Accession

Primary (citable) accession number: Q8KFG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program