Reviewed, UniProtKB/Swiss-Prot Q8KEY6 (HISX_CHLTE)

Names and origin

Protein names

Recommended name:
    Histidinol dehydrogenase
      Short name=HDH
    EC=1.1.1.23

Gene names

Name:	hisD
Ordered Locus Names:	CT0546

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine By similarity.
Catalytic activity	L-histidinol + 2 NAD+ = L-histidine + 2 NADH. HAMAP MF_01024
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9. HAMAP MF_01024
Sequence similarities	Belongs to the histidinol dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro histidinol dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Histidinol dehydrogenase HAMAP MF_01024		PRO_0000135754
Sites
	Active site	324	1	Proton acceptor By similarity
	Active site	325	1	Proton acceptor By similarity
	Metal binding	256	1	Zinc By similarity
	Metal binding	259	1	Zinc By similarity
	Metal binding	358	1	Zinc By similarity
	Metal binding	417	1	Zinc By similarity
	Binding site	126	1	NAD By similarity
	Binding site	188	1	NAD By similarity
	Binding site	211	1	NAD By similarity
	Binding site	234	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	325	1	Substrate By similarity
	Binding site	358	1	Substrate By similarity
	Binding site	412	1	Substrate By similarity
	Binding site	417	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KEY6-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 513422F758545954	FASTA	428	46,264
	10 20 30 40 50 60 MLTIYHFPQD EAALREQLNR TVSFDPDAQR TVDDILYRVR TEGDAAVLDY TERFQGIRLY 70 80 90 100 110 120 DMRVPEAEIE AAYAAADPEF IAILEEAFAN ITAFHRNEAE KSFFYEQKGG VILGQRVTPM 130 140 150 160 170 180 EKALLYVPGG KAAYPSSVLM NAAPAQVAGV DEISMTTPCD AEGKVNPHIL AAAKVAGITS 190 200 210 220 230 240 VYRLGGAQAV AAFAYGTATI PKVDIVTGPG NKYVALAKKQ VFGHVAIDSI AGPSEVVVIA 250 260 270 280 290 300 DAGAEPEFIV MDMFAQAEHD PDASAVLITP SAELADAVRE TAARLAGTML RGEVITRALT 310 320 330 340 350 360 DNGAIVVTGS MQEACKVSDM IAPEHLELHV DNPWEILPDL RHAGAIFMGQ WSCETVGDYF 370 380 390 400 410 420 AGPNHTLPTN GTARFFSPLS VRDFVKHTSI IAWSKSELAR TGEKIARFAD HEGLQAHAEA VRVRLKHL

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM71788.1.
RefSeq	NP_661446.1.
3D structure databases
HSSP	HSSP built from PDB template 1KAE based on UniProtKB P06988.
ModBase	Search...
Genome annotation databases
GeneID	1006212.
GenomeReviews	Gene locus CT0546 in contig AE006470_GR.
KEGG	cte:CT0546.
NMPDR	fig|194439.1.peg.540.
TIGR	CT0546.
Phylogenomic databases
HOGENOM	Q8KEY6.
OMA	Q8KEY6. LGVETFM.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0546-MON.
BRENDA	1.1.1.23. 189605.
Family and domain databases
HAMAP	MF_01024. [Tree]
InterPro	IPR001692. Histidinol_DH_CS. IPR012131. Hstdl_DH_prok-type. [Graphical view]
PANTHER	PTHR21256:SF2. Hstdl_DH_prok. 1 hit.
Pfam	PF00815. Histidinol_dh. 1 hit. [Graphical view]
PRINTS	PR00083. HOLDHDRGNASE.
ProDom	PD002680. Histidinol_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00069. hisD. 1 hit.
PROSITE	PS00611. HISOL_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HISX_CHLTE

Accession

Primary (citable) accession number: Q8KEY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)