ID   NADE_CHLTE              Reviewed;         277 AA.
AC   Q8KEX2;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=CT0560;
OS   Chlorobium tepidum.
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC   Chlorobaculum.
OX   NCBI_TaxID=1097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49652 / DSM 12025 / TLS;
RX   MEDLINE=22103685; PubMed=12093901; DOI=10.1073/pnas.132181499;
RA   Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M.,
RA   Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F.,
RA   Holt I.E., Umayam L.A., Mason T.M., Brenner M., Shea T.P.,
RA   Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B.,
RA   Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M.,
RA   Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M.;
RT   "The complete genome sequence of Chlorobium tepidum TLS, a
RT   photosynthetic, anaerobic, green-sulfur bacterium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; AE006470; AAM71802.1; -; Genomic_DNA.
DR   RefSeq; NP_661460.1; -.
DR   HSSP; P08164; 1IFX.
DR   GeneID; 1006143; -.
DR   GenomeReviews; AE006470_GR; CT0560.
DR   KEGG; cte:CT0560; -.
DR   NMPDR; fig|194439.1.peg.554; -.
DR   TIGR; CT0560; -.
DR   HOGENOM; Q8KEX2; -.
DR   OMA; Q8KEX2; CAINPIG.
DR   BioCyc; CTEP194439:CT_0560-MON; -.
DR   BRENDA; 6.3.1.5; 189605.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    277       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_0000152163.
FT   NP_BIND      36     43       ATP (By similarity).
FT   ACT_SITE     38     38       By similarity.
SQ   SEQUENCE   277 AA;  31125 MW;  CBEBD73BE5A462E2 CRC64;
     MKPQNLHFDY GLVEAILVPF IRNEIRKFGF GSVVLGLSGG IDSAVVCELA VRALGVENVL
     ALMMPYKTSS QESLDHAELM VDRLGIRYEI MPVTEVVDAF FATRPDASRL RRGNVMARSR
     MLCLYDVSAR DGCLVLGTSN KTELMLGYGT MFGDMASAVN PIGDLYKTQI FGLARHLGIP
     APLIDKPPSA DLWEGQSDEA DLGFSYEEVD QLLYMMLEER MDRDAILAEG IDSAFYQRVR
     SMVVRNQYKR MMPVIAKLSS RTPGIDFRYA RDWQEVR
//