Q8KEX2 (NADE_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	CT0560

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152163
Regions
	Nucleotide binding	36 – 43	8	ATP By similarity
Sites
	Active site	38	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KEX2 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: CBEBD73BE5A462E2	FASTA	277	31,125
	10 20 30 40 50 60 MKPQNLHFDY GLVEAILVPF IRNEIRKFGF GSVVLGLSGG IDSAVVCELA VRALGVENVL 70 80 90 100 110 120 ALMMPYKTSS QESLDHAELM VDRLGIRYEI MPVTEVVDAF FATRPDASRL RRGNVMARSR 130 140 150 160 170 180 MLCLYDVSAR DGCLVLGTSN KTELMLGYGT MFGDMASAVN PIGDLYKTQI FGLARHLGIP 190 200 210 220 230 240 APLIDKPPSA DLWEGQSDEA DLGFSYEEVD QLLYMMLEER MDRDAILAEG IDSAFYQRVR 250 260 270 SMVVRNQYKR MMPVIAKLSS RTPGIDFRYA RDWQEVR

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM71802.1.
RefSeq	NP_661460.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KEX2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006143.
GenomeReviews	Gene locus CT0560 in contig AE006470_GR.
KEGG	cte:CT0560.
NMPDR	fig|194439.1.peg.554.
PATRIC	21399201. VBIChlTep116050_0524.
TIGR	CT0560.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0560-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CHLTE

Accession

Primary (citable) accession number: Q8KEX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program