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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:CT0560
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521631 – 277NH(3)-dependent NAD(+) synthetaseAdd BLAST277

Interactioni

Protein-protein interaction databases

STRINGi194439.CT0560.

Structurei

3D structure databases

ProteinModelPortaliQ8KEX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238069.
KOiK01916.
OMAiQIGATYP.
OrthoDBiPOG091H00G3.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KEX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPQNLHFDY GLVEAILVPF IRNEIRKFGF GSVVLGLSGG IDSAVVCELA
60 70 80 90 100
VRALGVENVL ALMMPYKTSS QESLDHAELM VDRLGIRYEI MPVTEVVDAF
110 120 130 140 150
FATRPDASRL RRGNVMARSR MLCLYDVSAR DGCLVLGTSN KTELMLGYGT
160 170 180 190 200
MFGDMASAVN PIGDLYKTQI FGLARHLGIP APLIDKPPSA DLWEGQSDEA
210 220 230 240 250
DLGFSYEEVD QLLYMMLEER MDRDAILAEG IDSAFYQRVR SMVVRNQYKR
260 270
MMPVIAKLSS RTPGIDFRYA RDWQEVR
Length:277
Mass (Da):31,125
Last modified:October 1, 2002 - v1
Checksum:iCBEBD73BE5A462E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71802.1.
RefSeqiNP_661460.1. NC_002932.3.
WP_010932247.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71802; AAM71802; CT0560.
GeneIDi1006143.
KEGGicte:CT0560.
PATRICi21399201. VBIChlTep116050_0524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71802.1.
RefSeqiNP_661460.1. NC_002932.3.
WP_010932247.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KEX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT0560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM71802; AAM71802; CT0560.
GeneIDi1006143.
KEGGicte:CT0560.
PATRICi21399201. VBIChlTep116050_0524.

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238069.
KOiK01916.
OMAiQIGATYP.
OrthoDBiPOG091H00G3.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_CHLTE
AccessioniPrimary (citable) accession number: Q8KEX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.