Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42MagnesiumUniRule annotation1
Binding sitei118Deamido-NADUniRule annotation1
Binding sitei138ATPUniRule annotation1
Metal bindingi143MagnesiumUniRule annotation1
Binding sitei167ATPUniRule annotation1
Binding sitei189ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-585-MONOMER
UniPathwayiUPA00253; UER00333

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:CT0560
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521631 – 277NH(3)-dependent NAD(+) synthetaseAdd BLAST277

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0560

Structurei

3D structure databases

ProteinModelPortaliQ8KEX2
SMRiQ8KEX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K Bacteria
COG0171 LUCA
HOGENOMiHOG000238069
KOiK01916
OMAiCAINPIG
OrthoDBiPOG091H00G3

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00193 NadE_ammonia_dep, 1 hit
InterProiView protein in InterPro
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR022926 NH(3)-dep_NAD(+)_synth
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF02540 NAD_synthase, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KEX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPQNLHFDY GLVEAILVPF IRNEIRKFGF GSVVLGLSGG IDSAVVCELA
60 70 80 90 100
VRALGVENVL ALMMPYKTSS QESLDHAELM VDRLGIRYEI MPVTEVVDAF
110 120 130 140 150
FATRPDASRL RRGNVMARSR MLCLYDVSAR DGCLVLGTSN KTELMLGYGT
160 170 180 190 200
MFGDMASAVN PIGDLYKTQI FGLARHLGIP APLIDKPPSA DLWEGQSDEA
210 220 230 240 250
DLGFSYEEVD QLLYMMLEER MDRDAILAEG IDSAFYQRVR SMVVRNQYKR
260 270
MMPVIAKLSS RTPGIDFRYA RDWQEVR
Length:277
Mass (Da):31,125
Last modified:October 1, 2002 - v1
Checksum:iCBEBD73BE5A462E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM71802.1
RefSeqiNP_661460.1, NC_002932.3
WP_010932247.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM71802; AAM71802; CT0560
GeneIDi1006143
KEGGicte:CT0560
PATRICifig|194439.7.peg.524

Similar proteinsi

Entry informationi

Entry nameiNADE_CHLTE
AccessioniPrimary (citable) accession number: Q8KEX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health