Q8KER4 (ILVD_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	CT0619

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103457
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8KER4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A4C353EC88937ABD	FASTA	560	58,916
	10 20 30 40 50 60 MRSDTIKKGF EKAPHRSLLK ATGCVSTRDD FSKPFIGICN SFNELIPGHA HLQELGRIAK 70 80 90 100 110 120 EAVREAGGVP FEFNTIGVCD GIAMGHVGMR YSLASRELIA DSVETVVEAH RLDGLVCIPN 130 140 150 160 170 180 CDKITPGMMM GALRTNVPVV FVSGGPMKAG HTPSGKTVDL ISVFEAVGKC STGEITEDEL 190 200 210 220 230 240 QTVEECGCPG CGSCSGMFTA NSMNCLCEAL GFALPGNGTI LAADPRRNEL VKAAAGRIID 250 260 270 280 290 300 LVKKEVRPRQ ILTRTSMLNA FALDLAMGGS TNTILHTLAI ASEAELDFDF SELNDLSAKT 310 320 330 340 350 360 PYICKVSPAT TEVHIEDVDR AGGISAILKE LSKVEGLLDL SAPTVTGKTL GENIASAEVL 370 380 390 400 410 420 DRTVIRSVEE PYSTTGGLAV LYGNLAPNGA VVKTGAVSPA MMKHTGPAKV YDCQDDAIAG 430 440 450 460 470 480 IMNGDVKSGD VVVIRYEGPR GGPGMPEMLS PTSAIIGRGL GDSVALITDG RFSGGSRGAC 490 500 510 520 530 540 VGHVSPEAAD RGPIAAVQTG DMITIDIPAR SMTVALDDET IRQRIEALPK FEPKIKKGYL 550 560 ARYARMVTSA NTGAVLKNDF

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM71861.1.
RefSeq	NP_661519.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KER4.
ModBase	Search...
Proteomic databases
PRIDE	Q8KER4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1007743.
GenomeReviews	Gene locus CT0619 in contig AE006470_GR.
KEGG	cte:CT0619.
NMPDR	fig|194439.1.peg.613.
PATRIC	21399305. VBIChlTep116050_0576.
TIGR	CT0619.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0619-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CHLTE

Accession

Primary (citable) accession number: Q8KER4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program