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Protein

Thiazole synthase

Gene

thiG

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Schiff-base intermediate with DXPUniRule annotation1
Binding sitei159DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-723-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:CT0698
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001628041 – 259Thiazole synthaseAdd BLAST259

Proteomic databases

PRIDEiQ8KEJ1

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0698

Structurei

3D structure databases

ProteinModelPortaliQ8KEJ1
SMRiQ8KEJ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 186DXP bindingUniRule annotation2
Regioni207 – 208DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KEJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSLRLGTYT FSSRLILGTG KFSSTSAMIK AVRASGTQLV TVALRRFNRE
60 70 80 90 100
QAEDDLFGPL SEIEGLTLMP NTSGAATAKE AIKAAHIARE LSGSPFIKVE
110 120 130 140 150
IHPNPHHLMP DPIETWEACK ILAAEGFIVM PYIPADPVLA KRLEEVGCSS
160 170 180 190 200
VMPLGSAIGS GQGLSTAEMV KIIIRESSVP VIVDAGLRSP SEACAAMEMG
210 220 230 240 250
CEAVLVNSAV AVARDPAAMA LAFAKAVEAG FEARNAGLMP RSGSAVATSP

LTSFLGATR
Length:259
Mass (Da):27,070
Last modified:October 1, 2002 - v1
Checksum:i294E5DE2170F3474
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM71935.1
RefSeqiNP_661593.1, NC_002932.3
WP_010932380.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM71935; AAM71935; CT0698
GeneIDi1006326
KEGGicte:CT0698
PATRICifig|194439.7.peg.641

Entry informationi

Entry nameiTHIG_CHLTE
AccessioniPrimary (citable) accession number: Q8KEJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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