Reviewed, UniProtKB/Swiss-Prot Q8KEG8 (SAHH_CHLTE)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Synonyms:	sahH
Ordered Locus Names:	CT0721

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116956
Regions
	Region	223 – 391	169	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KEG8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 31B082405D241047	FASTA	471	51,949
	10 20 30 40 50 60 MTTEAAVLDY KVADISLAEW GRKEIEIAEK EMPGLMATRK KYEGKKPLAG ARIAGSLHMT 70 80 90 100 110 120 IQTAVLIETL VELGADVRWA SCNIFSTQDH AAAAIAAAGV PVFAWKGETL DEYWWCTRQI 130 140 150 160 170 180 LEFEGGLGPN LIVDDGGDAT LMIHFGYKIE NDPSMLDKTP GNAEEKALLQ QLKAVFAEDN 190 200 210 220 230 240 QRWHKVAAGM KGVSEETTTG VHRLYQMMEK GELLFPAINV NDSVTKSKFD NLYGCRESLA 250 260 270 280 290 300 DGIKRATDVM IAGKVVVVLG YGDVGKGCAH SMRSYGARVI VTEIDPICAL QAAMEGFEVT 310 320 330 340 350 360 TMEEAVKEGN IFVTATGNKD VITLDHIKQM RDEAIVCNIG HFDNEIQVDA LNNFKGATRI 370 380 390 400 410 420 NIKPQVDKYV FENGNCIYLL AEGRLVNLGC ATGHPSFVMS NSFTNQTLAQ IELWQNDYKV 430 440 450 460 470 GVYRLPKKLD EEVARLHLGQ IGAKLTTLTK EQADYIGVPV EGPYKPEHYR Y

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM71958.1.
RefSeq	NP_661616.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3R based on UniProtKB P10760.
ModBase	Search...
Genome annotation databases
GeneID	1006351.
GenomeReviews	Gene locus CT0721 in contig AE006470_GR.
KEGG	cte:CT0721.
NMPDR	fig|194439.1.peg.710.
TIGR	CT0721.
Phylogenomic databases
HOGENOM	Q8KEG8.
OMA	Q8KEG8. HMRAMKD.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0721-MON.
BRENDA	3.3.1.1. 189605.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR000043. Ad_hcy_hydrolase. IPR015878. Ado_hCys_hydrolase_NAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.1480. Ad_hcy_hydrolase. 1 hit.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CHLTE

Accession

Primary (citable) accession number: Q8KEG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)