Q8KEC3 (NUOA_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	CT0766

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 143	143	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000362659
Regions
	Transmembrane	7 – 27	21	Helical; Potential
	Transmembrane	63 – 83	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8KEC3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1312C1A2C3E66209	FASTA	143	16,002
	10 20 30 40 50 60 MDQTLSGFGT VFVFLVLGIV FVVGGYLTAR MLRPSRPNPE KNSTYECGEH AVGSAWVKFN 70 80 90 100 110 120 IRFYVVALIF IIFDVEVVFL YPWATVFKQL GAFALVEVLI FVGILVLGLV YAWVKGDLDW 130 140 VRPTPNIPKM PEMPVRRSGK ANG

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72003.1.
RefSeq	NP_661661.1. NC_002932.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006399.
GenomeReviews	Gene locus CT0766 in contig AE006470_GR.
KEGG	cte:CT0766.
NMPDR	fig|194439.1.peg.755.
PATRIC	21399563. VBIChlTep116050_0697.
TIGR	CT0766.
Phylogenomic databases
HOGENOM	HBG605540.
OMA	VKFNIRF.
ProtClustDB	CLSK637458.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0766-MONOMER.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_CHLTE

Accession

Primary (citable) accession number: Q8KEC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program