Q8KDK5 (PYRC_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	CT1042

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_B
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_B
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_B
Subunit structure	Homodimer By similarity. HAMAP MF_00220_B
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Dihydroorotase HAMAP MF_00220_B		PRO_0000147230
Sites
	Metal binding	65	1	Zinc 1 By similarity
	Metal binding	67	1	Zinc 1 By similarity
	Metal binding	183	1	Zinc 2 By similarity
	Metal binding	246	1	Zinc 2 By similarity
	Metal binding	321	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KDK5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 71FDCBCA995B7183	FASTA	439	47,039
	10 20 30 40 50 60 MSTLFLNARL LNPAENLDTV GSIKIGDDGL IEAVATGGES IPAKAEDNVI DLAGKVLAPG 70 80 90 100 110 120 LFDMHCHFRE PGQEYKETLE TGSAAAVAGG FTGVALMPNT RPVIDSPLGV AYIRHHSAGL 130 140 150 160 170 180 PIDLEVIGAM TVESRGEALA PYGKYASYSV KAVSDDGTAI QSSQIMRLAI EYAANFDLLL 190 200 210 220 230 240 IQHAEDKHLT AGGIMNDGAV SAMLGLKGIP EVAEPIMIAR DLQLIAWLKK HKLNGAVAEP 250 260 270 280 290 300 RYHVAHISTA ESVALVRKAK AAGLKVTCEV TPHHFTLTEH DLSSSIEKGN FIMKPPLASV 310 320 330 340 350 360 ENRDALIEGL RDGTIDAIAT DHAPHAKHEK ECPPDQAAFG IIGLETSLGL TITELVDKGV 370 380 390 400 410 420 ITLSQAIELL STNPRRIMGL ETILFRAGRK ANLTIIDPDC EWIVSESDFG SKSRNTPFMG 430 RKLKGRALGI YHNSKLIMR

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72275.1.
RefSeq	NP_661933.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KDK5.
ModBase	Search...
Proteomic databases
PRIDE	Q8KDK5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006973.
GenomeReviews	Gene locus CT1042 in contig AE006470_GR.
KEGG	cte:CT1042.
NMPDR	fig|194439.1.peg.1027.
PATRIC	21400077. VBIChlTep116050_0950.
TIGR	CT1042.
Phylogenomic databases
HOGENOM	HBG724623.
OMA	SHHQPHE.
ProtClustDB	PRK09357.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1042-MONOMER.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR00857. PyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_CHLTE

Accession

Primary (citable) accession number: Q8KDK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program