Reviewed, UniProtKB/Swiss-Prot Q8KDK5 (PYRC_CHLTE)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	CT1042

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Dihydroorotase HAMAP MF_00220		PRO_0000147230
Sites
	Metal binding	65	1	Zinc 1 By similarity
	Metal binding	67	1	Zinc 1 By similarity
	Metal binding	183	1	Zinc 2 By similarity
	Metal binding	246	1	Zinc 2 By similarity
	Metal binding	321	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KDK5-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 71FDCBCA995B7183	FASTA	439	47,039
	10 20 30 40 50 60 MSTLFLNARL LNPAENLDTV GSIKIGDDGL IEAVATGGES IPAKAEDNVI DLAGKVLAPG 70 80 90 100 110 120 LFDMHCHFRE PGQEYKETLE TGSAAAVAGG FTGVALMPNT RPVIDSPLGV AYIRHHSAGL 130 140 150 160 170 180 PIDLEVIGAM TVESRGEALA PYGKYASYSV KAVSDDGTAI QSSQIMRLAI EYAANFDLLL 190 200 210 220 230 240 IQHAEDKHLT AGGIMNDGAV SAMLGLKGIP EVAEPIMIAR DLQLIAWLKK HKLNGAVAEP 250 260 270 280 290 300 RYHVAHISTA ESVALVRKAK AAGLKVTCEV TPHHFTLTEH DLSSSIEKGN FIMKPPLASV 310 320 330 340 350 360 ENRDALIEGL RDGTIDAIAT DHAPHAKHEK ECPPDQAAFG IIGLETSLGL TITELVDKGV 370 380 390 400 410 420 ITLSQAIELL STNPRRIMGL ETILFRAGRK ANLTIIDPDC EWIVSESDFG SKSRNTPFMG 430 RKLKGRALGI YHNSKLIMR

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM72275.1.
RefSeq	NP_661933.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1006973.
GenomeReviews	Gene locus CT1042 in contig AE006470_GR.
KEGG	cte:CT1042.
NMPDR	fig|194439.1.peg.1027.
TIGR	CT1042.
Phylogenomic databases
HOGENOM	Q8KDK5.
OMA	SHHQPHE.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1042-MON.
BRENDA	3.5.2.3. 189605.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_CHLTE

Accession

Primary (citable) accession number: Q8KDK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)