Q8KDE2 (OTC_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	CT1112

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.
Caution	Lacks the conserved threonine and leucine residues in positions 66 and 276, respectively, which are part of the carbamoylphosphate and ornithine binding sites; they are replaced by a leucine and a methionine residue, respectively.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112907
Regions
	Region	65 – 69	5	Carbamoyl phosphate binding By similarity
	Region	274 – 277	4	Ornithine binding By similarity
Sites
	Binding site	116	1	Carbamoyl phosphate By similarity
	Binding site	143	1	Carbamoyl phosphate By similarity
	Site	37	1	Important for structural integrity By similarity
	Site	156	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KDE2 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 417082AB7115D812	FASTA	338	38,106
	10 20 30 40 50 60 MSQDNKGNGS KKRDFLGFTG LDAAKIIELF DYSLFIKQQR ETNRNSDEFR PIRHKTVAMI 70 80 90 100 110 120 FNKPSLRTRV SFELGVYELG GHAISLEGKS IGVGERESIE DIARLLSRYN DAIVARLHEH 130 140 150 160 170 180 EIIETLAKHA DIPVINALTD LSHPCQVLAD AFTLYEKGLW RDDIKVVFVG DGNNVANSWI 190 200 210 220 230 240 ELAGILPFHF VLACPEGYLP DETLLKQARS NAKGTIEILH DPMEAAKQAD VLYTDVWTSM 250 260 270 280 290 300 GQEEEMAERL KAFAPFQINA KMVAEAKPSA VIMHCMPAHR GQEISAEVMD GPQSIIIDEA 310 320 330 ENRLHVQKAL MVKLMNHDVY RKFHLTHRLH RAANRLKA

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72345.1.
RefSeq	NP_662003.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KDE2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006896.
GenomeReviews	Gene locus CT1112 in contig AE006470_GR.
KEGG	cte:CT1112.
NMPDR	fig|194439.1.peg.1097.
PATRIC	21400197. VBIChlTep116050_1010.
TIGR	CT1112.
Phylogenomic databases
HOGENOM	HBG579429.
OMA	SHDKFEA.
ProtClustDB	PRK00779.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1112-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. Divergent sequence. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_CHLTE

Accession

Primary (citable) accession number: Q8KDE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program