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Q8KCW4

- IMDH_CHLTE

UniProt

Q8KCW4 - IMDH_CHLTE

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei251 – 2511NADUniRule annotation
    Metal bindingi303 – 3031Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi305 – 3051Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei306 – 3061IMPUniRule annotation
    Active sitei308 – 3081Thioimidate intermediateUniRule annotation
    Metal bindingi308 – 3081Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei421 – 4211IMPUniRule annotation
    Metal bindingi475 – 4751Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi476 – 4761Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi477 – 4771Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi301 – 3033NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    BioCyciCTEP194439:GHN0-1329-MONOMER.
    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:guaBUniRule annotation
    Ordered Locus Names:CT1293
    OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
    Taxonomic identifieri194439 [NCBI]
    Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
    ProteomesiUP000001007: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Inosine-5'-monophosphate dehydrogenasePRO_0000093693Add
    BLAST

    Proteomic databases

    PRIDEiQ8KCW4.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi194439.CT1293.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8KCW4.
    SMRiQ8KCW4. Positions 2-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini93 – 15462CBS 1UniRule annotationAdd
    BLAST
    Domaini158 – 21760CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni341 – 3433IMP bindingUniRule annotation
    Regioni364 – 3652IMP bindingUniRule annotation
    Regioni388 – 3925IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    eggNOGiCOG0517.
    HOGENOMiHOG000165755.
    KOiK00088.
    OMAiHGHSKNI.
    OrthoDBiEOG6GTZPV.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8KCW4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKILYDALT FDDVLLVPAY SNVLPKETVV KSRLTRQIEV NIPLVSAAMD    50
    TVTEAELAIA LARAGGIGII HKNLSIDEQA RQVAKVKRFE SGIIRNPIHL 100
    FEDATIQDAI DLMIRHSISG IPVVEHPTPE GCLLLKGIVT NRDLRMTASS 150
    DEKITTIMTT NLVTAKEGID LLTAEDILMR NKIEKLLIID DNGYLKGLIT 200
    FKDIQKRKQC PDACKDSQGR LRAGAAVGIR ANTMSRVDAL VAAGVDVVAV 250
    DTAHGHSQAV LDMVATIKQK YPELQVIAGN VATPEAVRDL VKAGADAVKV 300
    GIGPGSICTT RIVAGVGMPQ LTAIMKCAEE AKKTDIPLIA DGGIKYSGDI 350
    AKALAAGADS VMMGSVFAGT DESPGETILY EGRRFKAYRG MGSLGAMSEP 400
    EGSSDRYFQD VSAETKKYVP EGIEGRIPAK GKLDEVVYQL IGGLKSAMGY 450
    CGVRTITELK ENTRFVRITS AGLRESHPHD VMITKEAPNY STSA 494
    Length:494
    Mass (Da):53,019
    Last modified:October 1, 2002 - v1
    Checksum:iCD4C06DBBCB724DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006470 Genomic DNA. Translation: AAM72523.1.
    RefSeqiNP_662181.1. NC_002932.3.
    WP_010932962.1. NC_002932.3.

    Genome annotation databases

    EnsemblBacteriaiAAM72523; AAM72523; CT1293.
    GeneIDi1006653.
    KEGGicte:CT1293.
    PATRICi21400541. VBIChlTep116050_1178.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006470 Genomic DNA. Translation: AAM72523.1 .
    RefSeqi NP_662181.1. NC_002932.3.
    WP_010932962.1. NC_002932.3.

    3D structure databases

    ProteinModelPortali Q8KCW4.
    SMRi Q8KCW4. Positions 2-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 194439.CT1293.

    Proteomic databases

    PRIDEi Q8KCW4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM72523 ; AAM72523 ; CT1293 .
    GeneIDi 1006653.
    KEGGi cte:CT1293.
    PATRICi 21400541. VBIChlTep116050_1178.

    Phylogenomic databases

    eggNOGi COG0517.
    HOGENOMi HOG000165755.
    KOi K00088.
    OMAi HGHSKNI.
    OrthoDBi EOG6GTZPV.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .
    BioCyci CTEP194439:GHN0-1329-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49652 / DSM 12025 / TLS.

    Entry informationi

    Entry nameiIMDH_CHLTE
    AccessioniPrimary (citable) accession number: Q8KCW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3