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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (guaB)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei251NADUniRule annotation1
Metal bindingi303Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi305Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei306IMPUniRule annotation1
Active sitei308Thioimidate intermediateUniRule annotation1
Metal bindingi308Potassium; via carbonyl oxygenUniRule annotation1
Active sitei406Proton acceptorUniRule annotation1
Binding sitei421IMPUniRule annotation1
Metal bindingi475Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi476Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi477Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi301 – 303NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGMP biosynthesis, Purine biosynthesis
LigandMetal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-1329-MONOMER
UniPathwayiUPA00601; UER00295

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:CT1293
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000936931 – 494Inosine-5'-monophosphate dehydrogenaseAdd BLAST494

Proteomic databases

PRIDEiQ8KCW4

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT1293

Structurei

3D structure databases

ProteinModelPortaliQ8KCW4
SMRiQ8KCW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 154CBS 1UniRule annotationAdd BLAST62
Domaini158 – 217CBS 2UniRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni341 – 343IMP bindingUniRule annotation3
Regioni364 – 365IMP bindingUniRule annotation2
Regioni388 – 392IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiENOG4105CP4 Bacteria
COG0516 LUCA
COG0517 LUCA
HOGENOMiHOG000165755
KOiK00088
OMAiGIGIVHK
OrthoDBiPOG091H02IM

Family and domain databases

CDDicd00381 IMPDH, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01964 IMPDH, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000644 CBS_dom
IPR005990 IMP_DH
IPR015875 IMP_DH/GMP_Rdtase_CS
IPR001093 IMP_DH_GMPRt
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00478 IMPDH, 1 hit
PIRSFiPIRSF000130 IMPDH, 1 hit
SMARTiView protein in SMART
SM00116 CBS, 2 hits
TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
PS00487 IMP_DH_GMP_RED, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KCW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKILYDALT FDDVLLVPAY SNVLPKETVV KSRLTRQIEV NIPLVSAAMD
60 70 80 90 100
TVTEAELAIA LARAGGIGII HKNLSIDEQA RQVAKVKRFE SGIIRNPIHL
110 120 130 140 150
FEDATIQDAI DLMIRHSISG IPVVEHPTPE GCLLLKGIVT NRDLRMTASS
160 170 180 190 200
DEKITTIMTT NLVTAKEGID LLTAEDILMR NKIEKLLIID DNGYLKGLIT
210 220 230 240 250
FKDIQKRKQC PDACKDSQGR LRAGAAVGIR ANTMSRVDAL VAAGVDVVAV
260 270 280 290 300
DTAHGHSQAV LDMVATIKQK YPELQVIAGN VATPEAVRDL VKAGADAVKV
310 320 330 340 350
GIGPGSICTT RIVAGVGMPQ LTAIMKCAEE AKKTDIPLIA DGGIKYSGDI
360 370 380 390 400
AKALAAGADS VMMGSVFAGT DESPGETILY EGRRFKAYRG MGSLGAMSEP
410 420 430 440 450
EGSSDRYFQD VSAETKKYVP EGIEGRIPAK GKLDEVVYQL IGGLKSAMGY
460 470 480 490
CGVRTITELK ENTRFVRITS AGLRESHPHD VMITKEAPNY STSA
Length:494
Mass (Da):53,019
Last modified:October 1, 2002 - v1
Checksum:iCD4C06DBBCB724DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM72523.1
RefSeqiNP_662181.1, NC_002932.3
WP_010932962.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM72523; AAM72523; CT1293
GeneIDi1006653
KEGGicte:CT1293
PATRICifig|194439.7.peg.1178

Similar proteinsi

Entry informationi

Entry nameiIMDH_CHLTE
AccessioniPrimary (citable) accession number: Q8KCW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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