Q8KCQ3 (LPXD_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	CT1360

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059664
Sites
	Active site	246	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KCQ3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: F33737215732FD65	FASTA	353	36,726
	10 20 30 40 50 60 MKIADIKAFL GRYFDPVELV GTGDIEIVGP AKIEEASTGH VSFVANEKYY RYIAQTGASL 70 80 90 100 110 120 VIVSQKAPLD DASPGTSFLK VADPYTAFVF ILQHFSGKRR IADTGIAASA SVAASVRLGE 130 140 150 160 170 180 NVSLGEHVVI GENCVIGDGT VIGPGTVLMD GVTVGSGCTI FPLVTIYDGT VIGDRVTIHS 190 200 210 220 230 240 GTVVGADGFG FAPQKDGSYI KIPQMGTVEI GDDVEIGANT TIDRATMGAT VIEKGAKIDN 250 260 270 280 290 300 LVQIAHNCRI GGDTVIASQA GISGSVKIGR QCLIGGQAGF AGHLELADRT SVAAKAGISK 310 320 330 340 350 SFLEPGLAIR GVPAQPMRDQ LRQEAQVRGL GEMKSKLEAL EAKLLALQQQ LGE

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72589.1.
RefSeq	NP_662247.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KCQ3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006735.
GenomeReviews	Gene locus CT1360 in contig AE006470_GR.
KEGG	cte:CT1360.
NMPDR	fig|194439.1.peg.1341.
PATRIC	21400665. VBIChlTep116050_1238.
TIGR	CT1360.
Phylogenomic databases
HOGENOM	HBG469615.
OMA	SYPAKIM.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1360-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_CHLTE

Accession

Primary (citable) accession number: Q8KCQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program