Reviewed, UniProtKB/Swiss-Prot Q8KCL0 (AROK_CHLTE)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	CT1405

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Shikimate kinase HAMAP MF_00109		PRO_0000237864
Regions
	Nucleotide binding	14 – 19	6	ATP By similarity
Sites
	Metal binding	18	1	Magnesium By similarity
	Binding site	36	1	Substrate By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	82	1	Substrate; via amide nitrogen By similarity
	Binding site	120	1	ATP By similarity
	Binding site	147	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KCL0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C59E7A41E5573F5E	FASTA	191	21,695
	10 20 30 40 50 60 MKHHSLIFLT GFSGSGKSTI GPLLANSLGF EFIDLDREIE LTAGKSINRI FAEDGEAAFR 70 80 90 100 110 120 SLELRTLEKI GQQERMVVSL GGGVLENDRC FELIRSHGTL IYLKSSPEIL TLRLQHKTDR 130 140 150 160 170 180 PLLKGPDGRK LTREEIQQRI AELLKKREPR YLKADLVLFT DSKKIGASVE ELTRKIERHI 190 RRASKNNTNE K

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM72633.1.
RefSeq	NP_662291.1.
3D structure databases
HSSP	HSSP built from PDB template 1L4U based on UniProtKB P95014.
ModBase	Search...
Genome annotation databases
GeneID	1006814.
GenomeReviews	Gene locus CT1405 in contig AE006470_GR.
KEGG	cte:CT1405.
NMPDR	fig|194439.1.peg.1385.
TIGR	CT1405.
Phylogenomic databases
HOGENOM	Q8KCL0.
OMA	PITRIFA.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1405-MON.
BRENDA	2.7.1.71. 189605.
Family and domain databases
HAMAP	MF_00109. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_CHLTE

Accession

Primary (citable) accession number: Q8KCL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)