Reviewed, UniProtKB/Swiss-Prot Q8KCJ0 (HEM2_CHLTE)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	hemB
Ordered Locus Names:	CT1431

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Enzyme regulation	Stimulated by magnesium, inhibited by zinc.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Miscellaneous	Does not seem to have a metal requirement for activity.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Delta-aminolevulinic acid dehydratase		PRO_0000140497
Sites
	Active site	253	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KCJ0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 55556CA0D0632148	FASTA	328	36,433
	10 20 30 40 50 60 MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE EVPSMPGSFR 70 80 90 100 110 120 FTIDRAVEEC KELYDLGIQA IDLFGIPEKK TEDGSEAYND NGILQQAIRA IKKAVPELCI 130 140 150 160 170 180 MTDVALDPFT PFGHDGLVRD GIILNDETVE VLQKMAVSHA EAGADFVSPS DMMDGRIGAI 190 200 210 220 230 240 REALDESDHS DVGILSYAAK YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTDEAMKEVE 250 260 270 280 290 300 LDIIEGADIV MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA NGWIDEERVM 310 320 MESLLCMKRA GADIIFTYYA KEAAKKLR

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72659.1.
RefSeq	NP_662317.1.
3D structure databases
SMR	Q8KCJ0. Positions 10-328.
ModBase	Search...
Genome annotation databases
GeneID	1005979.
GenomeReviews	Gene locus CT1431 in contig AE006470_GR.
KEGG	cte:CT1431.
NMPDR	fig|194439.1.peg.1411.
TIGR	CT1431.
Phylogenomic databases
HOGENOM	HBG285270.
OMA	DPFTSHG.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1431-MONOMER.
BRENDA	4.2.1.24. 189605.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_CHLTE

Accession

Primary (citable) accession number: Q8KCJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)