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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Enzyme regulationi

Stimulated by magnesium, inhibited by zinc.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200Schiff-base intermediate with substrateBy similarity1
Binding sitei210Substrate 1By similarity1
Binding sitei222Substrate 1By similarity1
Metal bindingi238MagnesiumBy similarity1
Active sitei253Schiff-base intermediate with substrateBy similarity1
Binding sitei279Substrate 2By similarity1
Binding sitei318Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:CT1431
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404971 – 328Delta-aminolevulinic acid dehydrataseAdd BLAST328

Proteomic databases

PRIDEiQ8KCJ0.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi194439.CT1431.

Structurei

3D structure databases

ProteinModelPortaliQ8KCJ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiENOG4105D52. Bacteria.
COG0113. LUCA.
HOGENOMiHOG000020323.
KOiK01698.
OMAiMDPANSN.
OrthoDBiPOG091H03SU.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR001731. ALAD.
IPR030656. ALAD_AS.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8KCJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE
60 70 80 90 100
EVPSMPGSFR FTIDRAVEEC KELYDLGIQA IDLFGIPEKK TEDGSEAYND
110 120 130 140 150
NGILQQAIRA IKKAVPELCI MTDVALDPFT PFGHDGLVRD GIILNDETVE
160 170 180 190 200
VLQKMAVSHA EAGADFVSPS DMMDGRIGAI REALDESDHS DVGILSYAAK
210 220 230 240 250
YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTDEAMKEVE LDIIEGADIV
260 270 280 290 300
MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA NGWIDEERVM
310 320
MESLLCMKRA GADIIFTYYA KEAAKKLR
Length:328
Mass (Da):36,433
Last modified:October 1, 2002 - v1
Checksum:i55556CA0D0632148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM72659.1.
RefSeqiNP_662317.1. NC_002932.3.
WP_010933098.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM72659; AAM72659; CT1431.
GeneIDi1005979.
KEGGicte:CT1431.
PATRICi21400789. VBIChlTep116050_1299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM72659.1.
RefSeqiNP_662317.1. NC_002932.3.
WP_010933098.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KCJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT1431.

Proteomic databases

PRIDEiQ8KCJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM72659; AAM72659; CT1431.
GeneIDi1005979.
KEGGicte:CT1431.
PATRICi21400789. VBIChlTep116050_1299.

Phylogenomic databases

eggNOGiENOG4105D52. Bacteria.
COG0113. LUCA.
HOGENOMiHOG000020323.
KOiK01698.
OMAiMDPANSN.
OrthoDBiPOG091H03SU.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR001731. ALAD.
IPR030656. ALAD_AS.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM2_CHLTE
AccessioniPrimary (citable) accession number: Q8KCJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not seem to have a metal requirement for activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.