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Q8KCJ0 (HEM2_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-aminolevulinic acid dehydratase

Short name=ALAD
Short name=ALADH
EC=4.2.1.24
Alternative name(s):
Porphobilinogen synthase
Gene names
Name:hemB
Ordered Locus Names:CT1431
OrganismChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS) [Reference proteome] [HAMAP]
Taxonomic identifier194439 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activity

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Enzyme regulation

Stimulated by magnesium, inhibited by zinc.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.

Subunit structure

Homooctamer By similarity.

Miscellaneous

Does not seem to have a metal requirement for activity.

Sequence similarities

Belongs to the ALADH family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   LigandMagnesium
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

porphobilinogen synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Delta-aminolevulinic acid dehydratase
PRO_0000140497

Sites

Active site2001Schiff-base intermediate with substrate By similarity
Active site2531Schiff-base intermediate with substrate By similarity
Metal binding2381Magnesium By similarity
Binding site2101Substrate 1 By similarity
Binding site2221Substrate 1 By similarity
Binding site2791Substrate 2 By similarity
Binding site3181Substrate 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8KCJ0 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 55556CA0D0632148

FASTA32836,433
        10         20         30         40         50         60 
MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE EVPSMPGSFR 

        70         80         90        100        110        120 
FTIDRAVEEC KELYDLGIQA IDLFGIPEKK TEDGSEAYND NGILQQAIRA IKKAVPELCI 

       130        140        150        160        170        180 
MTDVALDPFT PFGHDGLVRD GIILNDETVE VLQKMAVSHA EAGADFVSPS DMMDGRIGAI 

       190        200        210        220        230        240 
REALDESDHS DVGILSYAAK YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTDEAMKEVE 

       250        260        270        280        290        300 
LDIIEGADIV MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA NGWIDEERVM 

       310        320 
MESLLCMKRA GADIIFTYYA KEAAKKLR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006470 Genomic DNA. Translation: AAM72659.1.
RefSeqNP_662317.1. NC_002932.3.

3D structure databases

ProteinModelPortalQ8KCJ0.
SMRQ8KCJ0. Positions 10-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING194439.CT1431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM72659; AAM72659; CT1431.
GeneID1005979.
KEGGcte:CT1431.
PATRIC21400789. VBIChlTep116050_1299.

Phylogenomic databases

eggNOGCOG0113.
HOGENOMHOG000020323.
KOK01698.
OMAGEYAMVE.
OrthoDBEOG6VXFCB.
ProtClustDBPRK09283.

Enzyme and pathway databases

BioCycCTEP194439:GHN0-1470-MONOMER.
UniPathwayUPA00251; UER00318.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERPTHR11458. PTHR11458. 1 hit.
PfamPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSPR00144. DALDHYDRTASE.
SMARTSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM2_CHLTE
AccessionPrimary (citable) accession number: Q8KCJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2002
Last modified: November 13, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways