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Q8KCJ0

- HEM2_CHLTE

UniProt

Q8KCJ0 - HEM2_CHLTE

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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Enzyme regulationi

Stimulated by magnesium, inhibited by zinc.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001Schiff-base intermediate with substrateBy similarity
Binding sitei210 – 2101Substrate 1By similarity
Binding sitei222 – 2221Substrate 1By similarity
Metal bindingi238 – 2381MagnesiumBy similarity
Active sitei253 – 2531Schiff-base intermediate with substrateBy similarity
Binding sitei279 – 2791Substrate 2By similarity
Binding sitei318 – 3181Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-1470-MONOMER.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:CT1431
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Delta-aminolevulinic acid dehydratasePRO_0000140497Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi194439.CT1431.

Structurei

3D structure databases

ProteinModelPortaliQ8KCJ0.
SMRiQ8KCJ0. Positions 10-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
KOiK01698.
OMAiDMILTYF.
OrthoDBiEOG6VXFCB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8KCJ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE
60 70 80 90 100
EVPSMPGSFR FTIDRAVEEC KELYDLGIQA IDLFGIPEKK TEDGSEAYND
110 120 130 140 150
NGILQQAIRA IKKAVPELCI MTDVALDPFT PFGHDGLVRD GIILNDETVE
160 170 180 190 200
VLQKMAVSHA EAGADFVSPS DMMDGRIGAI REALDESDHS DVGILSYAAK
210 220 230 240 250
YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTDEAMKEVE LDIIEGADIV
260 270 280 290 300
MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA NGWIDEERVM
310 320
MESLLCMKRA GADIIFTYYA KEAAKKLR
Length:328
Mass (Da):36,433
Last modified:October 1, 2002 - v1
Checksum:i55556CA0D0632148
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006470 Genomic DNA. Translation: AAM72659.1.
RefSeqiNP_662317.1. NC_002932.3.
WP_010933098.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM72659; AAM72659; CT1431.
GeneIDi1005979.
KEGGicte:CT1431.
PATRICi21400789. VBIChlTep116050_1299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006470 Genomic DNA. Translation: AAM72659.1 .
RefSeqi NP_662317.1. NC_002932.3.
WP_010933098.1. NC_002932.3.

3D structure databases

ProteinModelPortali Q8KCJ0.
SMRi Q8KCJ0. Positions 10-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 194439.CT1431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM72659 ; AAM72659 ; CT1431 .
GeneIDi 1005979.
KEGGi cte:CT1431.
PATRICi 21400789. VBIChlTep116050_1299.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
KOi K01698.
OMAi DMILTYF.
OrthoDBi EOG6VXFCB.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
BioCyci CTEP194439:GHN0-1470-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / TLS.

Entry informationi

Entry nameiHEM2_CHLTE
AccessioniPrimary (citable) accession number: Q8KCJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not seem to have a metal requirement for activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3