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Q8KCJ0

- HEM2_CHLTE

UniProt

Q8KCJ0 - HEM2_CHLTE

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Enzyme regulationi

    Stimulated by magnesium, inhibited by zinc.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei200 – 2001Schiff-base intermediate with substrateBy similarity
    Binding sitei210 – 2101Substrate 1By similarity
    Binding sitei222 – 2221Substrate 1By similarity
    Metal bindingi238 – 2381MagnesiumBy similarity
    Active sitei253 – 2531Schiff-base intermediate with substrateBy similarity
    Binding sitei279 – 2791Substrate 2By similarity
    Binding sitei318 – 3181Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciCTEP194439:GHN0-1470-MONOMER.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:CT1431
    OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
    Taxonomic identifieri194439 [NCBI]
    Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
    ProteomesiUP000001007: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Delta-aminolevulinic acid dehydratasePRO_0000140497Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi194439.CT1431.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8KCJ0.
    SMRiQ8KCJ0. Positions 10-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OMAiDMILTYF.
    OrthoDBiEOG6VXFCB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8KCJ0-1 [UniParc]FASTAAdd to Basket

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    MSQLDLLNIV HRPRRLRRTA ALRNLVQENT LTVNDLVFPL FVMPGTNAVE    50
    EVPSMPGSFR FTIDRAVEEC KELYDLGIQA IDLFGIPEKK TEDGSEAYND 100
    NGILQQAIRA IKKAVPELCI MTDVALDPFT PFGHDGLVRD GIILNDETVE 150
    VLQKMAVSHA EAGADFVSPS DMMDGRIGAI REALDESDHS DVGILSYAAK 200
    YASSFYGPFR DALHSAPQFG DKSTYQMNPA NTDEAMKEVE LDIIEGADIV 250
    MVKPGLAYLD IVWRTKERFD VPVAIYHVSG EYAMVKAAAA NGWIDEERVM 300
    MESLLCMKRA GADIIFTYYA KEAAKKLR 328
    Length:328
    Mass (Da):36,433
    Last modified:October 1, 2002 - v1
    Checksum:i55556CA0D0632148
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006470 Genomic DNA. Translation: AAM72659.1.
    RefSeqiNP_662317.1. NC_002932.3.

    Genome annotation databases

    EnsemblBacteriaiAAM72659; AAM72659; CT1431.
    GeneIDi1005979.
    KEGGicte:CT1431.
    PATRICi21400789. VBIChlTep116050_1299.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006470 Genomic DNA. Translation: AAM72659.1 .
    RefSeqi NP_662317.1. NC_002932.3.

    3D structure databases

    ProteinModelPortali Q8KCJ0.
    SMRi Q8KCJ0. Positions 10-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 194439.CT1431.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM72659 ; AAM72659 ; CT1431 .
    GeneIDi 1005979.
    KEGGi cte:CT1431.
    PATRICi 21400789. VBIChlTep116050_1299.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OMAi DMILTYF.
    OrthoDBi EOG6VXFCB.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    BioCyci CTEP194439:GHN0-1470-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49652 / DSM 12025 / TLS.

    Entry informationi

    Entry nameiHEM2_CHLTE
    AccessioniPrimary (citable) accession number: Q8KCJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Does not seem to have a metal requirement for activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3