Q8KCE9 (PROA_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	CT1473

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189712

Sequences

	Sequence		Length	Mass (Da)
	Q8KCE9 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 246CFED586B56A43	FASTA	420	45,961
	10 20 30 40 50 60 MTEHEAIVKQ LQAVQNASRK IVPLNEETIN GLLVELADRI PAAADAILEA NRKDLERMDP 70 80 90 100 110 120 ADPRYDRLLL NEARLNSIAT DLRNVAALPS PLHRVLEERT LPNGLELKKV SVPLGVIGII 130 140 150 160 170 180 YESRPNVTFD VFALCLKSGN ATVLKGGSDA AYSNIAIVNL IQTVIRDRGL DPDMIYLLPA 190 200 210 220 230 240 EREAAHILLN AVGYIDVIIP RGSQALIDFA RKHSTVPVIE TGAGIVHTYF DQSGDLAMGR 250 260 270 280 290 300 DIVFNAKTRR PSVCNALDTL IVHESRIDDL PVLVVPLEEK NVQIFADEPA YYKLLGRYPD 310 320 330 340 350 360 ELLEMASPEH FGTEFLSLKM SIKTVGSLEE ALNHIARHSS KHSEAIIASD QTTIDAFMKQ 370 380 390 400 410 420 VDAAAVYANT STAFTDGAQF GLGAEIGIST QKLHARGPMA LKELCSYKWL VTGQGQVRTA

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72700.1.
RefSeq	NP_662358.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KCE9.
ModBase	Search...
Proteomic databases
PRIDE	Q8KCE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1006019.
GenomeReviews	Gene locus CT1473 in contig AE006470_GR.
KEGG	cte:CT1473.
NMPDR	fig|194439.1.peg.1452.
PATRIC	21400861. VBIChlTep116050_1335.
TIGR	CT1473.
Phylogenomic databases
HOGENOM	HBG318080.
OMA	HRIEAMA.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1473-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CHLTE

Accession

Primary (citable) accession number: Q8KCE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program