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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei323Coenzyme AUniRule annotation1
Binding sitei347Coenzyme AUniRule annotation1
Binding sitei512ATPUniRule annotation1
Binding sitei527ATPUniRule annotation1
Binding sitei535Coenzyme A; via carbonyl oxygenUniRule annotation1
Binding sitei538ATPUniRule annotation1
Metal bindingi549Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi551Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi554Magnesium; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi399 – 401ATPUniRule annotation3
Nucleotide bindingi423 – 428ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-1691-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Synonyms:acs
Ordered Locus Names:CT1652
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002083601 – 659Acetyl-coenzyme A synthetaseAdd BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei621N6-acetyllysineUniRule annotation1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ8KBY0

Interactioni

Protein-protein interaction databases

STRINGi194439.CT1652

Structurei

3D structure databases

ProteinModelPortaliQ8KBY0
SMRiQ8KBY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 208Coenzyme A bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQF Bacteria
COG0365 LUCA
HOGENOMiHOG000229981
KOiK01895
OMAiDHWWHDL
OrthoDBiPOG091H059D

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KBY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATEQTKGQS SESISSVLSE RRKFPPPEAF SSQSHISSME QYEKLYADAA
60 70 80 90 100
ADPDKYWGDL AEQFHWFKKW DSVLEWNAPY AKWFNGGTTN IAYNCLDVHV
110 120 130 140 150
GSWRKNKAAI IWEGEEGNER ILTYGELHRQ VSKFANVLKI AGIKPGDKVA
160 170 180 190 200
IYMGMVPELV IAVLACARVG AVHNVIFAGF AAHAITERVN DSRAKIVICA
210 220 230 240 250
DGTRRRGSTI NLKNIVDEAI INTPSVKNVI VLKVTGEEIH MHDGMDHWWH
260 270 280 290 300
DLMGLAVDEC EPAQVDSEHP LFLLYTSGST GKPKGILHTT AGYMVHAASS
310 320 330 340 350
FKYVFDIKDE DIYFCTADIG WITGHTYIIY GPLLNGATVF MYEGAPNYPQ
360 370 380 390 400
WDRFWDIINR HKITILYTAP TAIRAFIRAG NEWVTKHDLS SLRLLGTVGE
410 420 430 440 450
PINPEAWMWY HKYVGQEKCP IVDTWWQTET GGIMISPMPG ATPTKPGTAT
460 470 480 490 500
RPLPGIMVDV VRKDGTPCNA NEGGYLVVKR PWPSMLRTIY GDNERYEKTY
510 520 530 540 550
WSEFPGMYFT GDGARKDDDG YIWIMGRVDD VVNVSGHRLG TSEVESALVS
560 570 580 590 600
HEAVAEAAVV SRPDEIKGNA LVAFVTLKDG YEGDAKLRDS LGKHVAKEIG
610 620 630 640 650
AIAKPDEIRW AKGLPKTRSG KIMRRLLREL ATSNEIKGDV TTLEDLGVIE

NLREQEDEG
Length:659
Mass (Da):73,576
Last modified:September 26, 2003 - v2
Checksum:i2BB84C9AB13EA034
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM72877.1
RefSeqiNP_662535.2, NC_002932.3
WP_010933316.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM72877; AAM72877; CT1652
GeneIDi1006138
KEGGicte:CT1652
PATRICifig|194439.7.peg.1493

Similar proteinsi

Entry informationi

Entry nameiACSA_CHLTE
AccessioniPrimary (citable) accession number: Q8KBY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: March 28, 2018
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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