Reviewed, UniProtKB/Swiss-Prot Q8KBY0 (ACSA_CHLTE)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Synonyms:	acs
Ordered Locus Names:	CT1652

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208360
Sites
	Active site	529	1	By similarity
Amino acid modifications
	Modified residue	621	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KBY0-1 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: 2BB84C9AB13EA034	FASTA	659	73,576
	10 20 30 40 50 60 MATEQTKGQS SESISSVLSE RRKFPPPEAF SSQSHISSME QYEKLYADAA ADPDKYWGDL 70 80 90 100 110 120 AEQFHWFKKW DSVLEWNAPY AKWFNGGTTN IAYNCLDVHV GSWRKNKAAI IWEGEEGNER 130 140 150 160 170 180 ILTYGELHRQ VSKFANVLKI AGIKPGDKVA IYMGMVPELV IAVLACARVG AVHNVIFAGF 190 200 210 220 230 240 AAHAITERVN DSRAKIVICA DGTRRRGSTI NLKNIVDEAI INTPSVKNVI VLKVTGEEIH 250 260 270 280 290 300 MHDGMDHWWH DLMGLAVDEC EPAQVDSEHP LFLLYTSGST GKPKGILHTT AGYMVHAASS 310 320 330 340 350 360 FKYVFDIKDE DIYFCTADIG WITGHTYIIY GPLLNGATVF MYEGAPNYPQ WDRFWDIINR 370 380 390 400 410 420 HKITILYTAP TAIRAFIRAG NEWVTKHDLS SLRLLGTVGE PINPEAWMWY HKYVGQEKCP 430 440 450 460 470 480 IVDTWWQTET GGIMISPMPG ATPTKPGTAT RPLPGIMVDV VRKDGTPCNA NEGGYLVVKR 490 500 510 520 530 540 PWPSMLRTIY GDNERYEKTY WSEFPGMYFT GDGARKDDDG YIWIMGRVDD VVNVSGHRLG 550 560 570 580 590 600 TSEVESALVS HEAVAEAAVV SRPDEIKGNA LVAFVTLKDG YEGDAKLRDS LGKHVAKEIG 610 620 630 640 650 AIAKPDEIRW AKGLPKTRSG KIMRRLLREL ATSNEIKGDV TTLEDLGVIE NLREQEDEG

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM72877.1.
RefSeq	NP_662535.1.
3D structure databases
HSSP	HSSP built from PDB template 1PG4 based on UniProtKB Q8ZKF6.
ModBase	Search...
Genome annotation databases
GeneID	1006138.
GenomeReviews	Gene locus CT1652 in contig AE006470_GR.
KEGG	cte:CT1652.
NMPDR	fig|194439.1.peg.1629.
TIGR	CT1652.
Phylogenomic databases
HOGENOM	Q8KBY0.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1652-MON.
BRENDA	6.2.1.1. 189605.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_CHLTE

Accession

Primary (citable) accession number: Q8KBY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	September 26, 2003
Last modified:	June 16, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)